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A sperm drying liquid and a method for storing sperm

A drying liquid and sperm technology, applied in the field of biological cell preservation, can solve the problems of low embryonic development rate and achieve the effect of simple operation, high integrity and improved integrity

Inactive Publication Date: 2020-08-28
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the integrity of sperm genetic material is the prerequisite for fertilization and embryo development. In the prior art, the integrity of sperm after drying by evaporative drying method and the fertilization rate and embryo development rate after intracytoplasmic injection are lower than those of freeze-drying method.

Method used

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  • A sperm drying liquid and a method for storing sperm
  • A sperm drying liquid and a method for storing sperm
  • A sperm drying liquid and a method for storing sperm

Examples

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Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment provides a sperm drying solution, each liter of the sperm drying solution includes: 6.82g of sodium chloride, 0.28g of potassium chloride, 0.35g of anhydrous calcium chloride, 0.09g of magnesium chloride hexahydrate, 2.51g of sodium bicarbonate, Sodium phosphate 0.048g, glucose 1.08g, 4-hydroxyethylpiperazineethanesulfonic acid 2.76g, raffinose 10mmol and the rest of water.

[0035] This embodiment also provides a method for sperm preservation, including:

[0036] S1. Dilute the collected fresh semen with diluent until the density of sperm in the semen is 1×10 6 individual / mL.

[0037] S2. Centrifuge the above-mentioned diluted semen to remove the semen. The centrifugal factor of the centrifuge is 700g, and the centrifugation time is 3min.

[0038] S3. Mix the centrifuged semen with the above-mentioned dried sperm liquid to obtain a sperm suspension, and the sperm density in the sperm suspension is 1-2×10 6 individual / mL.

[0039] S4. Use a pipette g...

Embodiment 2

[0042] This embodiment provides a sperm drying solution, which has basically the same composition as the sperm drying solution provided in Example 1, the difference being that the amount of raffinose contained in each liter of the sperm drying solution is 20 mmol.

[0043] This embodiment also provides a sperm preservation method, which is the same as the sperm preservation method provided in Example 1, and details can be referred to in Example 1.

Embodiment 3

[0045] This embodiment provides a sperm drying solution, which has basically the same composition as the sperm drying solution provided in Example 1, the difference being that the amount of raffinose contained in each liter of the sperm drying solution is 50 mmol.

[0046] This embodiment also provides a sperm preservation method, which is the same as the sperm preservation method provided in Example 1, and details can be referred to in Example 1.

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PUM

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Abstract

A sperm drying liquid and a sperm preservation method, relating to the field of biological cell preservation, the sperm drying liquid can simulate the environmental state of the sperm growth state, can provide the basic needs of the sperm to survive in vitro and maintain a certain metabolic activity, and can better Protect the sperm and improve the integrity of the sperm. The sperm preservation method provided by the present invention adopts centrifugation to remove the semen in the semen, then mixes it with the above-mentioned sperm drying solution, and finally uses the evaporation drying method to dry. The whole process is simple to operate and does not require complex freezing and drying equipment. The treated sperm can be stored for a long time while still maintaining a high integrity. After long-term storage, it can be combined with egg cells to obtain a high cleavage rate and blastocyst development rate, especially suitable for field collection and collection of wild animal semen.

Description

technical field [0001] The invention relates to the field of biological cell preservation, in particular to a sperm drying liquid and a sperm preservation method. Background technique [0002] The preservation of semen is to prolong the survival time of sperm in vitro and maintain its fertilization ability, so that sperm can be transported for a long time and long distance without being limited by time and place. It is convenient to carry out inter-provincial and international exchanges and cooperation, is conducive to improving the utilization rate of good male animals and long-term preservation of genetic resources, and is of great significance in breeding conservation, variety improvement, introduction and human reproductive medicine. [0003] In the natural state, the life span of sperm is only a few hours after isolation, and it must undergo certain treatments before it can be transported over long distances. The traditional sperm preservation method is to freeze the s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 李晓霞刁云飞许保增王士勇李兆华常彤李丹丽陶蕾
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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