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Application of plant polyphenols in preparing pesticide for preventing Penicillium digitatum after harvesting of citrus

A technology for green mildew and citrus, which is applied to the application field of plant polyphenols in the preparation of medicines for preventing and controlling postharvest green mildew on citrus, can solve the problems of environmental and human health hazards, the problem of bacterial resistance, the decline of control effects, and the like. The effect of prolonging the storage period, easy operation and reducing the occurrence of green mold

Active Publication Date: 2017-06-13
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical fungicides such as thiabendazole and imazalil are mainly used in production to prevent and control citrus green mold. However, long-term use of these fungicides often leads to the problem of bacterial resistance, resulting in a gradual decline in the control effect, or even failure.
In addition, the long-term and large-scale use of chemical fungicides is likely to cause harm to the environment and human health

Method used

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  • Application of plant polyphenols in preparing pesticide for preventing Penicillium digitatum after harvesting of citrus
  • Application of plant polyphenols in preparing pesticide for preventing Penicillium digitatum after harvesting of citrus
  • Application of plant polyphenols in preparing pesticide for preventing Penicillium digitatum after harvesting of citrus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Experimental materials and methods

[0026] Experimental Materials:

[0027] Penicillium digitatum strain Penicillium digitatum was inoculated on the slant of potato dextrose agar medium (PDA, 200g potato, 20g glucose, 20g agar, 1L water), cultured at 25°C for 7 days, and stored at 4°C for later use.

[0028] Tannin was purchased from Tianjin Fuchen Chemical Reagent Factory.

[0029] experimental method:

[0030] 1. Colony growth inhibition test

[0031] The spore suspension of Penicillium digitatum (5 μl 1×10 6 spores / ml) were inoculated on PDA plates containing 200, 400, 600, 800 and 1000 μg / ml tannins, added an equal amount of sterile water as a control, and incubated at 25°C for 7 days. Colony diameter was determined by the cross method. Three replicates were set up for each experiment. Inhibition rate (%)=(water control colony diameter-tannin treated colony diameter) / water control colony diameter×100%.

[0032] Penicillium digitatum spore suspension (5 μl...

Embodiment 2

[0055] Choose Shatangju fruits that are free from injuries and pests. No pesticides were sprayed one month before harvesting. Soak it in 500μg / ml tannin aqueous solution for 2 minutes, put it in a cool and ventilated place to dry naturally, put it into a plastic box, and store it at room temperature. The incidence was counted after 7 days of storage. Each treatment was replicated three times, and each replicate processed 100 fruits. The 500 μg / ml aqueous tannin solution is obtained by placing 500 μg of tannin in 1 ml of water and mixing uniformly to obtain a 500 μg / ml aqueous tannin solution. The results of the storage experiment showed that the incidence rate of citrus green mold treated with clear water was 7.6%. The inhibitory rate of 500μg / ml tannin aqueous solution to citrus green mold was 70.3%, and the incidence rate of citrus green mold treated by it was only 2.3%.

Embodiment 3

[0057] Select citrus fruits whose maturity reaches commercial requirements, soak them in 200 μg / ml tannin aqueous solution for 3 minutes, place them in a cool and ventilated place to dry naturally, pack the single fruits in bags, and store them. The incidence was counted after 7 days of storage. Each treatment was replicated three times, and each replicate processed 100 fruits. The 200 μg / ml aqueous tannin solution is obtained by placing 200 μg of tannin in 1 ml of water and mixing uniformly to obtain a 200 μg / ml aqueous tannin solution. The results of storage experiments showed that. The inhibitory rate of 200μg / ml tannin to citrus green mold was better, and the incidence of citrus green mold was lower.

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Abstract

The invention discloses an application of plant polyphenols in preparing a pesticide for preventing Penicillium digitatum after harvesting of citrus and relates to an application of tannin in preparing a pesticide resisting Penicillium digitatum. The adopted tannin is natural polyphenol compounds, has a better inhibition function on spore germination and mycelial growth of Penicillium digitatum of the citrus, can remarkably reduce occurrence of Penicillium digitatum after harvesting of citrus fruits, effectively prolongs the storage period of the citrus fruits, does not affect the quality of the citrus fruits and has a good application prospect in the field of preservation of the citrus fruits.

Description

technical field [0001] The invention belongs to the field of prevention and control of fruit diseases and insect pests, and in particular relates to the application of plant polyphenols in the preparation of medicaments for preventing and treating postharvest green mold of citrus. [0002] Background technique: [0003] Citrus green mold is the most important disease of postharvest citrus fruit, and the loss caused by it accounts for more than 90% of the total loss. At present, chemical fungicides such as thiabendazole and imazalil are mainly used in production to prevent and control citrus green mold. However, long-term use of these fungicides often leads to the problem of bacterial resistance, resulting in a gradual decline in the control effect, or even failure. In addition, the long-term use of large quantities of chemical fungicides is likely to cause harm to the environment and human health. [0004] Tannins are natural polyphenols produced by a class of higher plants....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P3/00A23B7/154
CPCA01N43/16A23B7/154
Inventor 朱从一雷梦英李建雄
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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