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Preparation method of low-antigen corneal stromal lens processed by X-ray irradiation

A technology of corneal stroma and lens, which is applied in the field of corneal stroma lens preparation, can solve the problems of refractive correction degree, inability to be implanted in the interlayer of cornea, and poor flexibility. burden effect

Active Publication Date: 2019-08-16
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Freeze-drying is a commonly used method of decellularization. This method reduces physical and chemical damage, and also reduces the denaturation of corneal tissue proteins. However, it has a certain impact on the migration and growth of corneal stromal cells, and there are problems in the degree of postoperative refractive correction.
After decellularization of corneal materials by chemical and physical methods, the corneal layer may not be able to be implanted due to poor flexibility, and the microenvironment of the corneal stroma may also be changed, resulting in graft failure.

Method used

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  • Preparation method of low-antigen corneal stromal lens processed by X-ray irradiation
  • Preparation method of low-antigen corneal stromal lens processed by X-ray irradiation
  • Preparation method of low-antigen corneal stromal lens processed by X-ray irradiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of Human Corneal Stratum Lens Treated by X-ray Irradiation

[0019] Using laser femtosecond technology to personalize the design of incision, curvature, and diopter, and take out the corneal stroma lens, which can be derived from myopia patients undergoing SMILE surgery or human allogeneic cornea. The operations were performed by the same experienced chief physician. The corneal stromal lens removed during the operation was stored in a wet room at 4°C. Quickly irradiate the freshly preserved corneal stromal lens with RS2000 biological X-ray irradiator. The total irradiation dose is 100Gy, and the irradiation parameters are: 160kV, 25mA, 0.3mm copper filter. After irradiation, corneal stromal lenses were stored in sterile glycerol at -20°C.

[0020] Using the TUNEL staining technique to detect the cell apoptosis in the lens shows that after X-ray irradiation, all the cells in the lens are apoptotic, showing red fluorescence, see figure 1 .

Embodiment 2

[0021] Example 2 Preparation of Rabbit Corneal Stratum Lens Treated by X-ray Irradiation

[0022] The lamellar corneal stroma lens was taken out from the rabbit cornea by laser femtosecond technology, and a certain diopter of the taken out stroma layer was set. Fix the rabbit body with a rabbit bag, expose the head, spread the surgical drapes, place it on the VisuMax full femtosecond laser operating table, use the VisuMax femtosecond laser to perform double-layer scanning in the corneal stroma of the monkey eye to be subjected to SMILE surgery, and make Corneal stroma lens.

[0023] The operations were performed by the same experienced chief physician. The corneal stromal lens removed during the operation was stored in a wet room at 4°C. Quickly irradiate the freshly preserved corneal stromal lens with RS2000 biological X-ray irradiator. The total irradiation dose is 100Gy, and the irradiation parameters are: 160kV, 25mA, 0.3mm copper filter. After irradiation, corneal stro...

Embodiment 3

[0024] Example 3 Rhesus monkey heterogeneous lamellar keratoplasty experiment using X-ray irradiated human corneal stromal lens

[0025] The human lamellar corneal stromal lens prepared in Example 1 was used as a medical donor material (donor for short), and healthy rhesus monkeys were selected as recipients for lamellar keratoplasty. Preparations before routine corneal refractive surgery. After general anesthesia with ketamine hydrochloride injection and chlorpromazine, the rhesus monkey body was fixed with a monkey bag, the head was exposed, surgical drapes were spread, and placed on the VisuMax femtosecond laser operating table , using the VisuMax femtosecond laser to perform double-layer scanning in the corneal stroma of monkeys undergoing SMILE to make lamellar pockets of corneal stroma. The operation was performed by the same experienced physician. The donor was implanted into the corneal stromal pocket with microscopic toothless forceps and a separating spatula, and th...

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Abstract

The invention discloses a low-antigen corneal stromal lens processed by X-ray irradiation and a preparation method thereof, comprising the following steps: 1) obtaining fresh lamellar corneal material, and taking out the corneal stromal lens material from a donor by femtosecond laser technology ; 2) Store the removed corneal stroma lens material in a wet room with a set temperature; 3) Quickly take out the corneal stroma lens for X-ray irradiation with a dose of 100Gy; 4) Store the corneal stroma lens after X-ray irradiation in a dry place bacteria glycerin. X-ray irradiated heterogeneous or allogeneic corneal stromal lens assisted by femtosecond laser maintains the good transparency and tissue compatibility of the implant, and the use of X-ray irradiated heterogeneous biological corneal stromal lens material greatly reduces corneal supply. To reduce the burden on the body, the use of femtosecond laser-assisted transplantation makes the operation method safer and more efficient. At the same time, the transplanted lens can accurately control the diopter, so as to achieve the purpose of personalized design treatment.

Description

technical field [0001] The invention relates to the technical field of tissue engineering cornea, including the preparation and application of a corneal stromal lens. Background technique [0002] Corneal disease is the second most common eye disease in the world after cataract. At present, there are more than 10 million binocular corneal blind patients in the world, and there are 1.5 to 2 million new cases of corneal blindness every year. Corneal transplantation has been the most effective solution for many corneal lesions. However, in recent years, corneal materials that can be donated and applied clinically have become more and more scarce, and it is difficult to meet the needs of the public. [0003] In recent years, researchers from various countries are working hard to find a safe and efficient transplant material to relieve the tension of corneal donors and reduce the rejection of surgical transplants. As early as 1963, McCarey studied the use of lead oxide glass i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/16A61L27/36A61L27/50
CPCA61F2/16A61F2240/002A61L27/3604A61L27/3691A61L27/50A61L2430/16
Inventor 钟兴武
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
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