Novel diagnosis and treatment agent with integrated functions of copper ion fluorescence detection and drug therapy and preparation method of novel diagnosis and treatment agent

Abstract

The invention discloses a novel diagnosis and treatment agent with integrated functions of copper ion fluorescence detection and drug therapy and a preparation method of the novel diagnosis and treatment agent. According to the novel diagnosis and treatment agent, an energy donor and an energy receptor are combined together through the interactionof covalent bonds, the energy donor is an up-conversion luminescence nanocrystalline (UCNPs) with a core-shell structure, one end of the nanocrystalline is provided with -N=C=0 groups, the energy receptor is a rhodamine b derivative for singly detecting copper ions, and the surface of the UCNPs is further loaded with mesoporous silica as a drug loading channel; and the UCNPs is subjected to mesoporous silica coating by virtue of a reverse micelle method and is hydrolyzed with3-(triethoxy silyl)propyl isocyanate with one end provided with silicon hydroxyl, the energy donor and an energy receptor dye molecule with one end provided with amino are subjected to bonded through the covalent bonds, and finally a duct of mesoporous silica is loaded with a drug molecule DOX by virtue of an electrostatic effect and a hydrogen bond. The novel diagnosis and treatment agent has high sensitivity and selectivity and low background fluorescence influence.

Description

Technical field [0001] The invention relates to a novel nano-diagnostic and therapeutic agent, in particular to a novel nano-diagnostic and therapeutic agent used for the integration of copper ion fluorescence detection and drug therapy functions. Background technique [0002] Copper is an essential trace element in the human body. Excessive intake of copper can damage the human nervous system, causing symptoms such as memory loss, distraction, and emotional instability. Therefore, clarifying the physiological process and the distribution of copper in cells is of great significance for studying the complex physiological functions of copper. Therefore, the development of copper ion analysis and detection technology is extremely important. [0003] Currently detecting Cu 2+ The main methods include atomic absorption, colorimetry, fluorescence quenching, etc. However, some of the above methods require large instruments, some require professionals, and some have higher detection limit...

AI Technical Summary

Problems solved by technology

[0003] Current detection of Cu 2+ The methods mainly include atomic absorption method, colorimetric method, fluorescence quenching method, etc., but some of the above methods require large instruments, some require professionals, and some have higher detection limits.

Commonly used probe materials mainly include: organic molecular fluorescent probes, quantum dots, etc., but they have the following disadvantages: (1) In order to improve selectivity, most copper ion probes are detected in organic solvents or components containing organic solvents. , poor water solubility, cannot be applied in pure aqueous environment or cell system

(2) Shortcomings such as insensitive detection, long detection time, and inability to detect quickly

But while chemotherapy kills cancer cells, it can also cause serious damage to normal cells

At the same time, the diagnosis and treatment of a single drug therapy are independent of each other. The two medications not only prolong the treatment cycle, but also increase the risk of treatment and the pain of the patient, and it is not easy to reach the lesion.

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