Preparation method of nano Gd-MOFs for magnetic resonance imaging

A kind of magnetic resonance imaging and nanotechnology, applied in the direction of nanotechnology for sensing, preparations for in vivo experiments, nanotechnology, etc., can solve the adverse effects of MRI contrast agent use, Gd-MOFs nanorod toxicity, application Obstacles and other problems, to achieve obvious effect, large relaxation rate, good biocompatibility

CN106822926AInactive Publication Date: 2017-06-13WUHAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2017-06-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of nano Gd-MOFs for magnetic resonance imaging. The preparation method of the nano Gd-MOFs comprises the following steps: (1) dissolving a metal salt, a ligand and a terminating agent in a solvent, and then mixing to obtain a mixed solution; (2) stirring the obtained mixed solution at room temperature, placing in a water bath, carrying out a stirring reaction, then placing in an oven, allowing to stand, centrifuging and washing, and dispersing and preserving by a solvent, to obtain oil-phase dispersed Gd-MOFs; (3) taking a certain amount of the Gd-MOFs obtained in the step (2), centrifuging to remove the solvent, modifying by an ethanol solution containing triethylamine, and finally, dispersing in water to obtain the nano Gd-MOFs for magnetic resonance imaging. Compared with the prior art, the preparation method is simple in preparation process and mild in reaction conditions. The prepared nano Gd-MOFs have the particle size of 45-60 nm, are near spherical, have uniform particle size distribution, and are more suitable for phagocytosis; in an ultra high field intensity of 11.7 T, the transverse relaxation rate r2 is measured to be 96.8 mM<-1>*s<-1>, and the magnetic relaxation rate is high; the disadvantage of high cytotoxicity of the nano Gd-MOFs is overcome; when the concentration of Gd is 0.4 mmol / L or less, the cell close packing T2 weighted imaging effect is obvious.
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Description

technical field

[0001] The invention belongs to the field of magnetic resonance imaging contrast agents, and in particular relates to a preparation method of nanometer Gd-MOFs for magnetic resonance imaging. Background technique

[0002] Metal-organic frameworks (MOFs) are unique hybrid porous materials assembled from metal ions and organic groups. Compared with traditional porous materials, MOFs have multiple advantages, including large surface area, tunable pore size and shape, tunable composition and structure, biodegradability, and various functionalizations, which make MOFs promising for catalytic applications. , Selective adsorption separation and gas storage and other fields. Due to the tunability of MOFs, nanoscale metal-organic frameworks (nanoscale Metal-orannic frameworks, NMOFs) can be synthesized by rationally designing the structure and adjusting the synthesis conditions. Using paramagnetic metals such as gadolinium as metal ion connection points, NMOFs prepa...

Examples

Embodiment Construction

[0033] Embodiments of the present invention will be described in detail below in conjunction with examples. Those skilled in the art will understand that the following examples are only preferred examples of the present invention, so as to better understand the present invention, and thus should not be considered as limiting the scope of the present invention.

[0034] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the experimental materials used, unless otherwise specified, were purchased from conventional biochemical reagent manufacturers.

[0035] In the following examples, a desktop high-speed centrifuge (XiangYi TG16-WS) was used for centrifugation; the SEM scanning electron microscope photos were obtained by using a Quanta FEG 250 field emission environment scanning electron microscope.

[0036] (1) Dissolve 0.1mmol (20mg) trimellitic acid in 8ml DMF, fully dissolve, and record it as solution A; mix 1mmol 1-ethyl...