Lung cancer related gene mutation detection method, primer and reagent
A detection method and technology for lung cancer, which are applied in biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of large sample size, high false negative rate, and complicated operation of multiple mutation sites.
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[0108] Example 1: Detection method of lung cancer related gene mutation based on Ion Torrent sequencing platform
[0109] This embodiment adopts the following reagents: 5×Ion Ampliseq TM HiFi Master Mix (Life Company), 2 x Ion Ampliseq TM Primer Pool (Life Corporation), FuPa Reagent (Life Corporation), Ion P1Adapter and IonXpress TM Barcode X (Life Corporation), Switch Solution (Life Corporation), DNA Ligase (Life Corporation), XP Reagent (Beckman Corporation), Platium TMPCR SuperMix High Fidelity (Life Company), Library Amplification Primer Mix (Life Company), Nuclease-FreeWater, absolute ethanol.
[0110] Before the experiment of this method, a reagent needs to be freshly prepared: 75% ethanol.
[0111] Instruments and equipment needed for this experiment: centrifuge, magnetic stand, pipette, PCR instrument, oscillator, fluorometer Qubit3.0.
[0112] Main experimental steps:
[0113] (1) gDNA extraction of samples to be tested
[0114] In this example, the gDNA sa...
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