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A Morphological Observation Method for Comparison of Differences Between Cannabis Varieties

A difference and morphological technology, which is applied in the preparation of test samples, scientific instruments, and material analysis through optical means, can solve the unreported morphological differences among cannabis varieties, the inability to obtain variety parent information, and new Inconvenient breeding work and other problems, to achieve the effect of high safety, strong operability, and high experimental efficiency

Active Publication Date: 2019-03-26
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the germplasm resources are introduced, it is often impossible to obtain the parental information of the introduced varieties, which leads to the fact that the appearance and technical characteristics of the introduced cannabis varieties through different channels may be very similar, or even the situation of close relatives of the same variety, which brings great difficulties to the breeding of new varieties. It is a big inconvenience, and correspondingly increases the consumption of scientific research resources
Therefore, in order to improve the utilization rate of scientific research resources, select suitable parents, and improve the efficiency of new variety breeding, it is necessary to use a simple, fast, and low-cost evaluation method to conduct a preliminary evaluation of the differences between cannabis varieties. There is no report on the evaluation method of the difference

Method used

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  • A Morphological Observation Method for Comparison of Differences Between Cannabis Varieties
  • A Morphological Observation Method for Comparison of Differences Between Cannabis Varieties
  • A Morphological Observation Method for Comparison of Differences Between Cannabis Varieties

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0051] Specific embodiment 1: This embodiment provides a comparative morphological observation method for the differences of cannabis varieties. The specific implementation steps are as follows:

[0052] 1. Obtain observation plants

[0053] Choose 5 cannabis plants that are grown under the same cultivation conditions and are expected to be compared when they are in the same development (such as cannabis varieties that are very similar in appearance and process characteristics or need to be judged whether they are related species) as materials. The development period can be selected, the seedling stage (4 to 6 leaf stage), budding stage, blooming stage and process maturity stage.

[0054] 2. Collect morphological data

[0055] For the convenience of description, the material location is divided into 4 areas. Area A: 1 / 3 of the bottom of the cannabis stem; Area B: 1 / 2 of the cannabis stem; Area C: 1 / 3 of the cannabis stem; Area D: the top of the cannabis stem. The seedling leaf chara...

specific Embodiment approach 2

[0100] Embodiment 2: This embodiment provides a comparative morphological observation method for the differences of cannabis varieties. The specific implementation steps are as follows:

[0101] 1. Obtain observation plants

[0102] Collected 5 hemp plants in the full bloom period of "Hema No. 1" and "Silesia" grown under the same cultivation conditions.

[0103] 2. Collect morphological data

[0104] (1) Observation methods and indicators of shape data

[0105] (a) Observation method of shape data:

[0106] The shape data is measured with tape measure and vernier caliper.

[0107] (b) Observation indicators:

[0108] Plant height, diameter of zone A, diameter of zone B, node length of zone B, diameter of zone C, leaf length, middle lobe length, middle lobe width, petiole length.

[0109] (2) Observation methods and indicators of microstructure data

[0110] (a) Material:

[0111] Cut 3cm long stalks from the A, B, and C areas of the stem respectively, and cut the stalks from the top of the p...

specific Embodiment approach 3

[0130] Specific implementation mode 3: This implementation mode provides a comparative morphological observation method for the differences of cannabis varieties. The specific implementation steps are as follows:

[0131] 1. Obtain observation plants

[0132] Collect 5 sprouting plants of the hemp variety "Silesia" and "Glion" grown under the same cultivation conditions.

[0133] 2. Collect morphological data

[0134] (1) Observation methods and indicators of shape data

[0135] (a) Observation method of shape data:

[0136] The shape data is measured with tape measure and vernier caliper.

[0137] (b) Observation indicators:

[0138] Plant height, diameter of zone A, diameter of zone B, node length of zone B, diameter of zone C, leaf length, middle lobe length, middle lobe width, petiole length.

[0139] (2) Observation methods and indicators of microstructure data

[0140] (a) Material:

[0141] Cut 3cm long stalks from the A, B, and C areas of the stems respectively, and cut the stalks fro...

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Abstract

The invention discloses a morphological observation method for intervarietal difference comparison of cannabis. The method comprises the following steps: firstly, acquiring observed plants; secondly, collecting morphological data of the plants; thirdly, performing data conversion; fourthly, comparing intervarietal difference of the cannabis. The method has the following advantages that: 1, the method is applicable to rapid comparison of the intervarietal difference; 2, the experimental efficiency is high, the operation method is simple and convenient, and the operability is high; 3, the experiment cost is low, only one set of microscopic imaging system with a measuring function is required in the aspect of instruments, and only a double-edge blade, ethyl alcohol, formaldehyde, glacial acetic acid, glycerinum and distilled water are involved in the tools and reagents of an experiment; 4, if the ethyl alcohol is used as a fixing liquid, then a toxic chemical is not used in the whole experiment process, so that the safety is high.

Description

Technical field [0001] The invention belongs to the technical field of plant morphology, and relates to a method for evaluating the morphological differences between cannabis varieties. Background technique [0002] Cannabis sativa L., also known as hemp, hemp, etc., is an annual erect herb of the Cannabinaceae family (Cannabinaceae). It was originally produced in Sikkim, Bhutan and other countries, but it is now wild or cultivated in various countries, and cultivated in various parts of our country. According to the content of tetrahydrocannabinol in the plant and its use, hemp can be divided into three categories: fiber, seed (THC<0.3%), and medicinal (THC>0.3%). Hemp fiber is the earliest used fiber in human history. It has high tenacity and can be decomposed naturally, making it an excellent textile material. The clothes woven with hemp fibers have excellent qualities such as antibacterial, deodorant, moisture wicking, sun-proof and anti-corrosion, and good UV protect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N1/30G01N1/28
CPCG01N1/2813G01N1/30G01N21/84G01N2001/305G01N2021/8466
Inventor 郭梦桥王晓楠程薪宇孙宇峰夏尊民徐海军曹焜
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI