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Quick tissue treatment reagent for pathological section

A technology for tissue processing and pathological sectioning, which is applied in the field of clinical pathological diagnostic reagents, which can solve problems such as long tissue processing time, harmful working and living environments, and large reagent volumes, shortening diagnostic time, reducing physical hazards, and improving diagnostic speed Effect

Inactive Publication Date: 2017-06-16
王瑞才
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are following deficiencies in this method: the formalin, xylene etc. that adopt are volatile, have strong irritation and high acute toxicity, are all very harmful to the working environment and living environment of pathological staff, long-term contact with formalin Marin may cause chronic respiratory diseases, cancer, memory and intelligence decline, and in severe cases, gastrointestinal perforation, liver damage and even coma; the tissue processing process is carried out step by step, and each process requires a large amount of reagents , the organization takes a long time to process, and the harm to the operator is great

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In the embodiment of the present invention, a rapid tissue processing reagent for pathological slices, the raw materials in parts by weight include: 60 parts of propylene glycol, 5 parts of dodecyl ethoxy sulfobetaine, 10 parts of acetone, 1 part of carrageenan 1 part of sodium chloride, 1 part of disodium edetate, 0.5 part of diethylamine, 1 part of glacial acetic acid, and 20 parts of distilled water.

[0016] During preparation, the above-mentioned raw materials are weighed according to parts by weight, various liquids are put into beakers in turn, an electromagnetic rod is put into the liquid, and stirred on an electromagnetic cooker for 15 minutes to obtain the product.

Embodiment 2

[0018] In the embodiment of the present invention, a rapid tissue processing reagent for pathological slices, the raw materials in parts by weight include: 150 parts of propylene glycol, 30 parts of dodecyl ethoxy sulfobetaine, 40 parts of acetone, 15 parts of carrageenan 10 parts of sodium chloride, 8 parts of disodium edetate, 10 parts of diethylamine, 15 parts of glacial acetic acid, and 100 parts of distilled water.

[0019] During preparation, the above-mentioned raw materials are weighed according to parts by weight, various liquids are put into beakers in turn, an electromagnetic rod is put into the liquid, and stirred on an electromagnetic cooker for 40 minutes to obtain the product.

Embodiment 3

[0021] In the embodiment of the present invention, a rapid tissue processing reagent for pathological slices, the raw materials in parts by weight include: 70 parts of propylene glycol, 8 parts of dodecyl ethoxy sulfobetaine, 12 parts of acetone, 1 part of carrageenan 1 part of sodium chloride, 1 part of disodium edetate, 1 part of diethylamine, 1 part of glacial acetic acid, and 25 parts of distilled water.

[0022] During preparation, the above-mentioned raw materials are weighed according to parts by weight, various liquids are put into beakers in turn, an electromagnetic rod is put into the liquid, and stirred on an electromagnetic cooker for 20 minutes to obtain the product.

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PUM

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Abstract

The invention discloses a quick tissue treatment reagent for a pathological section. The quick tissue treatment reagent is prepared from the following raw materials in parts by weight: 60 to 150 parts of propylene glycol or isopropanol, 5 to 30 parts of dodecyl ethoxy sulfonyl betaine or sorbitan laurate, 10 to 40 parts of acetone, 1 to 15 parts of carrageenan, 1 to 10 parts of sodium chloride, 1 to 8 parts of an anticoagulant, 0.5 to 10 parts of amine, 1 to 15 parts of glacial acetic acid and 20 to 100 parts of distilled water. The quick tissue treatment reagent is obtained by respectively weighing all the raw materials, putting various types of liquid into a flask in sequence, putting an electromagnetic stick into the liquid, and carrying out stirring on an electromagnetic oven for 15 to 40 minutes. The quick tissue treatment reagent disclosed by the invention can complete fixing, dewatering and clarification one-step treatment on a pathological tissue, so that the diagnosis speed is increased, and a result can be obtained after 3 to 5 hours; the pain of a patient waiting to see a doctor is greatly relieved, and a clinical doctor can take corresponding treatment measures in time. The quick tissue treatment reagent does not contain formaldehyde and benzene, so that the harm to the health of technical personnel of the pathology department and the pollution to the environment are greatly reduced.

Description

technical field [0001] The invention relates to the field of clinical pathological diagnostic reagents, in particular to a rapid tissue processing reagent for pathological slices. Background technique [0002] Pathological cell preparation technology is an integral part of pathological diagnosis, and the quality of preparation technology directly affects whether pathologists can make correct pathological diagnosis. Paraffin section method is the most widely used method in routine histological preparation techniques. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science. method. But there are following deficiencies in this method: the formalin, xylene etc. that adopt are volatile, have strong irritation and high acute toxicity, are all very harmful to the working environment and living en...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/30G01N2001/307
Inventor 王瑞才
Owner 王瑞才
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