Compound insecticide of beauveria bassiana and osthole
A technology of Beauveria bassiana and osthole, which is applied in the field of pesticides, can solve the problems of undiscovered Beauveria bassiana insecticides, etc., and achieve the goal of delaying the occurrence of insecticide resistance, significant synergistic effect, and good control effect Effect
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Embodiment 1
[0021] Example 1: Screening of the mixing ratio of Beauveria bassiana and osthole
[0022] The test object was Bemisia tabaci ( Bemisia tabaci ) second-instar nymphs, the test agents were Beauveria bassiana, osthole, and a mixed preparation of Beauveria bassiana and osthole. Beauveria bassiana is 20 billion spores / gram of pure sporopollen, and osthole is the original drug with a content of 98%.
[0023] The test method refers to the agricultural industry standard of the People's Republic of China "Pesticide Indoor Bioassay Test Guidelines" insecticide NY / T 1154.9-2008, and the test adopts the spray treatment method. A series of concentrations of Beauveria bassiana spore suspension, osthole, and a mixed preparation of Beauveria bassiana and osthole were sprayed on cucumber leaves with second-instar nymphs of Bemisia tabaci with a small sprayer, and the control was Sterile water, dry naturally. There were 3 leaves per treatment, 100 nymphs per leaf, and 3 repetitions. The p...
Embodiment 2
[0035] Example 2: 10% Beauveria bassiana osthole wettable powder and its control effect on whitefly on cucumber in greenhouse
[0036] Beauveria bassiana pure sporopollen (20 billion spores / g) 9%, osthole (98% technical grade) 1%, glucose (powder) 2%, sodium lignosulfonate 1%, starch 3%, Dispersant BD55 3%, washing powder 3%, diatomaceous earth supplemented to 100%, prepared 10% Beauveria bassiana osthole wettable powder. The mixed preparation is diluted 2000 times with water, and sprayed to control whitefly on cucumbers in greenhouses ( Bemisia tabaci ), when the temperature in the greenhouse is 26°C and the relative humidity is 85%, the cumulative mortality rate of pests in 7 days reaches 98.7%.
Embodiment 3
[0037] Example 3: 10% Beauveria bassiana osthole wettable powder and its control effect on yellow thrips on cucumber in greenhouse
[0038]Pure pollen of Beauveria bassiana (20 billion spores / g) 9%, osthole (98% technical grade) 1%, glucose (powder) 2%, sodium dodecylsulfonate 1%, starch 3 %, dispersant WLND100 3%, diatomaceous earth to make up to 100%, made 10% Beauveria bassiana osthole wettable powder. The mixed preparation is diluted 2000 times with water, and sprayed to prevent and control yellow thrips ( Thrips flavus ), when the temperature in the greenhouse is 26°C and the relative humidity is 85%, the cumulative mortality rate of pests in 7 days reaches 90.1%.
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