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A kind of highly active peanut chitosan and its preparation method

A technology of active polysaccharide and peanut shell, applied in biochemical equipment and methods, glycosylase, pharmaceutical formulations, etc., can solve the problems of long extraction time, low extraction efficiency, low energy consumption, etc., and improve the stability of enzyme activity. , The effect of improving hydrolysis activity and saving extraction time

Active Publication Date: 2020-07-14
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the extraction efficiency is low, the extraction time is long, and the energy consumption is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The washed and dried peanut shells are pulverized by an ultrafine pulverizer to obtain peanut shell powder, which is mixed with water at a ratio of 1:20, and 0.9 g of endoglucanase mutant and exocellulase are added to every 50 g of peanut shells. The complex enzyme composed of mutants, the compounding ratio of endoglucanase mutants and exocellulase mutants is 5:2, and ultrasonically assisted enzymolysis extraction at 65°C for 10 minutes. The resulting mixture was subjected to vacuum filtration, and the filtrate was retained. The filtrate was vacuum rotary evaporated to obtain a concentrated solution; after the concentrated solution was cooled, pre-cooled ethanol was added, and placed at 4°C for 12 hours, the precipitate was collected by centrifugation, and the supernatant was retained.

[0025] The obtained precipitate was redissolved with water, the supernatant was collected by centrifugation, pre-cooled ethanol was added, and placed at 4°C for 12 hours, the precipitat...

Embodiment 2

[0028]The washed and dried peanut shells are pulverized by an ultrafine pulverizer to obtain peanut shell powder, which is mixed with water at a ratio of 1:15, and 0.65 g of endoglucanase mutant and exocellulase are added to every 50 g of peanut shells. The complex enzyme composed of mutants, the compounding ratio of endoglucanase mutants and exocellulase mutants is 2:1, microwave-assisted enzymolysis and extraction at a temperature of 70°C for 12 minutes. The resulting mixture was subjected to vacuum filtration, and the filtrate was retained. The filtrate was vacuum rotary evaporated to obtain a concentrated solution; after the concentrated solution was cooled, pre-cooled ethanol was added, and placed at 4°C for 12 hours, the precipitate was collected by centrifugation, and the supernatant was retained.

[0029] The obtained precipitate was redissolved with water, the supernatant was collected by centrifugation, pre-cooled ethanol was added, and placed at 4°C for 12 hours, th...

Embodiment 3

[0032] The washed and dried peanut shells are pulverized by an ultrafine pulverizer to obtain peanut shell powder, which is mixed with water at a ratio of 1:30, and 1.5 g of endoglucanase mutant and exocellulase are added to every 50 g of peanut shells. The complex enzyme composed of mutants, the compounding ratio of endoglucanase mutants and exocellulase mutants is 3:2, microwave-assisted enzymolysis and extraction at a temperature of 79°C for 8 minutes. The resulting mixture was subjected to vacuum filtration, and the filtrate was retained. The filtrate was vacuum rotary evaporated to obtain a concentrated solution; after the concentrated solution was cooled, pre-cooled ethanol was added, and placed at 4°C for 12 hours, the precipitate was collected by centrifugation, and the supernatant was retained.

[0033] The obtained precipitate was redissolved with water, the supernatant was collected by centrifugation, pre-cooled ethanol was added, and placed at 4°C for 12 hours, the...

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Abstract

The invention discloses a high-activity peanut shell polysaccharide and a preparation method thereof and belongs to the technical field of natural product preparation. Mutants of an endoglucanase and a cellobiohydrolase are obtained by a site-directed mutation way, compounding is performed, and an active polysaccharide and a flavonoid compound are synchronously obtained from peanut shells by an ultrasonic assisted enzymolysis and extraction way. The optimum hydrolysis temperature and enzyme activity of the complex enzymes are improved by the complex enzymes and the preparation process, operation steps are simplified, extraction time is reduced, synchronous preparation of the flavonoid compound and the water-soluble polysaccharide from peanut shells is realized, synchronous enzymolysis and extraction of the polysaccharide under a medium-high temperature condition is also realized, the obtained active polysaccharide is high in flavonoid content, and strong in antioxidant activity and antibacterial activity, and has a plurality of physiological functions and a wide application range.

Description

technical field [0001] The invention relates to a high-activity peanut chitosan and a preparation method thereof, belonging to the technical field of natural product preparation. Background technique [0002] Polysaccharides are generally a natural substance that exists in living organisms, directly participate in the basic metabolic process of organisms, and have a positive impact on the metabolic process of organisms. It has significant physiological functions such as preventing cardiovascular disease, preventing diabetes, removing harmful substances from the human body, and improving human immunity. It can be used as a functional food for preventing, alleviating symptoms, and assisting in the treatment of cardiovascular and other diseases. It can also be widely used in food, health products, and pharmaceutical products as a food additive or nutritional fortifier to supplement the amount of dietary fiber required by the human body, increase Product health function, improv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/04C12N9/42A23L33/105A61K36/48A61P31/12A61P31/00A61P37/04A61P29/00
CPCA23V2002/00A61K36/48C12N9/2437C12P19/04C12P19/14C12Y302/01004C12Y302/01091A23V2250/21
Inventor 孙杰毕洁张初署于丽娜王明清徐同城杨伟强张建成
Owner SHANDONG PEANUT RES INST
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