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Method for rapidly flaking hibiscus rosa-sinensis fiber

A fibrous, fast technology, applied in the field of biological non-slicing slices, which can solve the problems of difficult to observe, incomplete fiber structure, long time consumption, etc.

Active Publication Date: 2017-06-20
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of isolating hibiscus fiber mainly adopts 10% potassium dichromate and 10% nitric acid isolation method, but using this method, it takes a long time to isolate, and the obtained fiber structure is incomplete, which is difficult to observe under a biological microscope to its structure such as clear cell walls and pits

Method used

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  • Method for rapidly flaking hibiscus rosa-sinensis fiber
  • Method for rapidly flaking hibiscus rosa-sinensis fiber
  • Method for rapidly flaking hibiscus rosa-sinensis fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Use scissors to cut the old branches of hibiscus more than two years old, peel off the bark, soak the bark in a mixed solution of 15% potassium dichromate and 8% nitric acid by mass percentage, boil for 60min, and then add 10% by mass Hydrogen peroxide and 50% acetic acid mixture 20ml, continue to boil for 30min. Use tweezers to clip the treated plant tissue into a petri dish filled with clear tap water, pick out the plant fiber with a dissecting needle, place it on a clean glass slide, and drop 0.5-1ml of 0.1% safranin staining solution Dyeing for 10min. Then wash off excess safranin staining solution with 70% alcohol, then add 0.5-1ml of 50% glycerol dropwise, and cover with a cover slip to complete the mounting. Observe the plant fiber structure under an electron microscope. The result is as Picture 1-1 , Figure 1-2 , Figure 1-3 .

Embodiment 2

[0024] Use scissors to cut the old branches of hibiscus more than two years old, peel off the bark, soak the bark in a mixed solution of 20% potassium dichromate and 10% nitric acid by mass percentage, boil for 90min, and then add 12% by mass Hydrogen peroxide and 60% acetic acid mixture 10ml, continue to boil for 30min. Use tweezers to clip the treated plant tissue into a petri dish filled with clear tap water, pick out the plant fiber with a dissecting needle, place it on a clean glass slide, and drop 0.5-1ml of 0.1% safranin staining solution Dyeing for 10min. Then wash off excess safranin staining solution with 70% alcohol, then add 0.5-1ml of 50% glycerol dropwise, and cover with a cover slip to complete the mounting. Observe the plant fiber structure under a biological microscope. Observe the plant fiber structure under a biological microscope. The result obtained by this method is not much different from the result obtained in Example 1.

Embodiment 3

[0026] Cut the old branches of Hibiscus hibiscus more than two years with scissors, peel off the bark, soak the bark in the mixed solution of 25% potassium dichromate and 15% nitric acid by mass percentage, boil for 60-90min, and then add the mass percentage of Pour off the mixed solution of 25% potassium dichromate and 15% nitric acid, then add 10-20ml of mixed solution of 15% hydrogen peroxide and 65% acetic acid in mass percentage, and continue boiling treatment for 30-60min. Use tweezers to clip the treated plant tissue into a petri dish filled with clear tap water, pick out the plant fiber with a dissecting needle, place it on a clean glass slide, and drop 0.5-1ml of 0.1% safranin staining solution Dyeing for 10min. Then wash off excess safranin staining solution with 70% alcohol, then add 0.5-1ml of 50% glycerol dropwise, and cover with a cover slip to complete the mounting. Observe the plant fiber structure under a biological microscope. The result obtained by this me...

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Abstract

The invention relates to a method for rapidly flaking hibiscus rosa-sinensis fiber. The method comprises the following steps of firstly boiling mixed liquor of potassium dichromate with the mass percent being 15 percent to 25 percent and nitric acid with the mass percent being 8 percent to 15 percent for processing peeled fresh plant tree barks; adding mixed liquor of hydrogen peroxide with the mass percent being 10 percent to 15 percent and acetic acid with the mass percent being 50 percent to 65 percent for boiling and treating the tree barks; putting the treated tree barks into distilled water, and picking plant fiber out; finally staining with sarranine and flaking for observation. According to the method provided by the invention, a potassium dichromate-nitric acid treatment method and a hydrogen peroxide-glacial acetic acid treatment method are combined, so that the plant fiber is quickly acquired. According to a maceration method, compared with an original method, the time is greatly shortened, the obtained fiber has a complete structure, and clear structures such as cell walls and pits can be observed through an optical microscope.

Description

technical field [0001] The invention belongs to the technical field of biological non-slicing sheeting, and relates to a method for fast sheeting of hibiscus fibers. Background technique [0002] Plant fibers are commonly found in the vegetative organs of plants. They are a part of plant mechanical tissues and an important part of plant transportation system. They are the main places for plant material transportation and maintenance of normal life activities. There are many types of plant fibers. According to the location of the fibers, plant fibers can be divided into wood fibers, hypodermis fibers, bast fibers, cortex fibers, pericycle fibers, and ring pith fibers; fibers can be classified according to their special organizational forms. Divided into crystal sheath fiber, embedded crystal fiber, crystal-containing fiber, separator fiber, branched fiber, etc. The plant fibers usually referred to mainly refer to wood fibers, which include all elongated sclerenchyma tissues ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31G01N1/28
CPCG01N1/2813G01N1/31
Inventor 周琼裴艳艳辛佳佳陈书权陆志森韦远涛胡姗姗
Owner GUANGXI UNIV
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