Trichoderma*brassinolide wettable powder as well as preparation method and application thereof

A technology of brassinolide and wettable powder is applied in the field of Trichoderma·brassinolide wettable powder and preparation thereof, and can solve the problem that the control effect is inferior to the control effect of chemical fungicides, and the biological control and pollution-free, Active substances are easily affected by the environment, etc., to achieve the effect of promoting photosynthesis, convenient preparation and easy coating

Active Publication Date: 2017-06-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the application of these compound preparations: one, the control effect of Trichoderma compound preparations is still not as good as that of chemical fungicides, and various chemical fungicides have inhibitory effects on the growth of Trichoderma itself, indirectly affecting the growth of Trichoderma. The biocontrol effect of mold
When applied in the field, the stability of Trichoderma is poor, and the active substance is easily affected by the environment
2. The compounding of Trichoderma and chemical pesticides still pollutes the environment to a certain extent, and does not achieve the purpose of biological control without pollution
3. Trichoderma wettable powder produced in the domestic market still has a part of precipitation after being dissolved in water, and the suspension rate is low

Method used

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  • Trichoderma*brassinolide wettable powder as well as preparation method and application thereof
  • Trichoderma*brassinolide wettable powder as well as preparation method and application thereof
  • Trichoderma*brassinolide wettable powder as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] This embodiment provides a preparation method of Trichoderma brassinolide wettable powder, the specific steps are as follows:

[0037] 1. Plate strain activation: inoculate Trichoderma dark green CGMCC No.6479 in a PDA petri dish, incubate upside down in an incubator at 28°C for 4-5 days; then inoculate Botrytis cinerea in a PDA petri dish, and incubate at 25°C They were cultured upside down in the box for 7 days, and light and dark were alternately treated.

[0038] The PDA medium is: 200g of potato boiled and filtered upper layer juice, 20g of glucose, 20g of agar powder, purified water to 1L, each 250mL is divided into 500mL Erlenmeyer flasks, and autoclaved at 121°C for 25min.

[0039] 2. Preparation of primary strains: first prepare PD medium: 200g potatoes, 20g glucose, 1L purified water, each 250mL is dispensed into a 500mL Erlenmeyer flask, and autoclaved at 121°C for 25min. Then, insert the activated plate bacteria in step 1 into the PD medium, place 180r / min,...

Embodiment 2

[0059] This example investigates the control effects of different concentration ratios of two effective components of the wettable powder of the present invention on tomato botrytis cinerea, as shown in Table 4 and Table 5. The result of table 4 shows that when Trichoderma spore concentration is 10 8 When CFU / g, 0.1% brassinolide was diluted 10 times, both of them had the best inhibitory effect on Botrytis cinerea mycelium growth, and the bacteriostatic rate could reach 73.6%, and the bacteriostatic rate was higher than other treatment groups. Further, the research results in Table 5 show that the concentration of Trichoderma spores is between 7.5-8.0×10 8 Brassinolide 1- 3mL, the two together have a more obvious inhibitory effect on Botrytis cinerea, with a control effect of 74.84%. Compared with other ingredients, the difference is significant.

[0060] Table 4 Effects of different concentrations of Trichoderma and brassinolide on the growth of Botrytis cinerea

[0061] ...

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Abstract

The invention discloses a trichoderma*brassinolide wettable powder as well as a preparation method and application thereof. The wettable powder is prepared from powder obtained by mixing a sporular hyphal homogenate of trichoderma, brassinolide and food-grade hydroxy starch ether, wherein the mass ratio of the sporular hyphal homogenate of the trichoderma to the food-grade hydroxy starch ether in the powder is 2 to 1; the wettable powder is also prepared from dextrin, sodium lignosulphonate, a dispersing agent NNO (Sodium Methylenebisnaphthalenesulfonate), methylcellulose, carboxymethylcellulose and chitosan. The wettable powder provided by the invention has the advantages of being convenient to confect and easy to coat, being high-efficiency and low-toxicity, being relatively safe to an environment, and having a favorable prevention effect on botrytis cinerea.

Description

technical field [0001] The invention relates to a Trichoderma brassinolide wettable powder and its preparation method and application, in particular to a preparation method of a biocontrol Trichoderma wettable powder and its application in the control of tomato gray mold. Background technique [0002] As a very important biocontrol fungus in biological control, Trichoderma has a variety of antagonistic mechanisms, and it has a good inhibitory effect on various pathogenic fungi such as Fusarium, Botrytis cinerea, and Rhizoctonia solani. In this way, Trichoderma can systemically induce plant defense responses, thereby improving plant disease resistance. However, there are two problems that need to be solved in the application of Trichoderma: Trichoderma sometimes produces some inhibitory substances to inhibit seed germination, so the growth-promoting function of plant-derived hormones can solve this problem. On the other hand, among the biological agents of Trichoderma, the l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N25/14A01N63/04A01N43/22A01P3/00A01P21/00
CPCA01N25/10A01N25/14A01N43/22A01N63/30A01N2300/00
Inventor 张婧迪陈捷林润泽刘志诚王猛孙佳楠贺安乐
Owner SHANGHAI JIAO TONG UNIV
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