Method for preparing polyphenol from Chinese white olive dregs

A technology of olive pomace and proportions, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, food science, etc., can solve the problems of not many olive polyphenols, not being suitable for industrial production, and restricting wide application, etc. Simple and economical, highly reproducible, and high sample recovery results

Inactive Publication Date: 2017-06-27
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with water extraction, the organic solvent extraction method consumes less energy, has a higher extraction rate, and can effectively reduce the denaturation of polyphenols during the extraction process, but the residual organic solvent and the pressure on the environment have always restricted this method. widespread application of the law
[0005] At present, there are not many published data on the separation and purification of olive polyphenols, and there is no data suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing polyphenols from olive pomace, comprising the steps of:

[0016] (1) Mix 150g of olive pomace dry powder with 750mL of ethanol in proportion, stir, extract for 4 hours, stand still, filter, extract twice, combine the extracts, and evaporate and dry the extracts at 40°C; the ethanol is the volume ratio 99.0:1.0 methanol and concentrated hydrochloric acid mixture;

[0017] (2) Mix the product obtained in step (1) with petroleum ether in a ratio of 1g: 5mL, extract 3 times, combine the supernatant and recover petroleum ether; add the extract in a ratio of 1g: 5mL Ethyl acetate was extracted three times, and the supernatants were combined and evaporated at 40°C to evaporate the ethyl acetate, and the solid was frozen and freeze-dried to obtain the crude olive pomace polyphenol extract. The extraction rate is 91.2%.

[0018] Olive pomace polyphenol crude extract can be purified by various known methods, and the following methods are not limiting the ...

Embodiment 2

[0024] (1) Mix 150g of olive pomace dry powder with 700mL of ethanol in proportion, stir, extract for 5 hours, stand still, filter, extract once, and evaporate the extract to dryness at 35°C;

[0025] (2) Mix the product obtained in step (1) with petroleum ether in a ratio of 1g: 4mL, extract 4 times, combine the supernatant and recover petroleum ether; add the extract in a ratio of 1g: 4mL Ethyl acetate, extracted 4 times, the combined supernatants were evaporated at 35°C to evaporate the ethyl acetate, the solid was frozen and freeze-dried to obtain the crude olive pomace polyphenol extract. The extraction rate is 89.9%.

[0026] Olive pomace polyphenol crude extract can be purified by various known methods, and the following methods are not limiting the present invention:

[0027] (1) According to the proportion, dissolve 100mg olive pomace polyphenol crude extract in 45mL ethanol, and filter with 45μm organic filter membrane;

[0028] (2) Pump the filtrate into the pretr...

Embodiment 3

[0033] A method for preparing polyphenols from olive pomace, comprising the steps of:

[0034] (1) Mix 150g of olive pomace dry powder with 800mL of ethanol in proportion, stir, extract for 3 hours, stand still, filter, extract for 3 times, combine the extracts, and evaporate and dry the extracts at 45°C;

[0035] (2) Mix the product obtained in step (1) with petroleum ether in a ratio of 1g: 6mL, extract twice, combine the supernatant and recover petroleum ether; add the extract in a ratio of 1g: 6mL Ethyl acetate, extracted twice, the combined supernatants were evaporated at 45°C to evaporate the ethyl acetate, the solid was frozen and freeze-dried to obtain the crude olive pomace polyphenol extract. The extraction rate is 90.6%.

[0036] Olive pomace polyphenol crude extract can be purified by various known methods, and the following methods are not limiting the present invention:

[0037] (1) According to the proportion, dissolve 100mg olive pomace polyphenol crude extra...

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PUM

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Abstract

The invention discloses a method for preparing polyphenols from olive pomace, which comprises the following steps: (1) mixing olive pomace dry powder with ethanol, stirring, extracting, standing still, filtering, extracting, combining extracts, and evaporating and drying; (2) Mix the product obtained in step (1) with petroleum ether, extract, supernatant and recover petroleum ether; add the extract to ethyl acetate, extract, combine the supernatant to evaporate ethyl acetate, freeze the solid and freeze-dry to obtain olives Slag polyphenol crude extract. The olive polyphenol extract is extracted from olive pomace, has a simple preparation method, high extraction efficiency, low production cost and high safety, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of polyphenol preparation, and in particular relates to a method for preparing polyphenol from olive pomace. Background technique [0002] Polyphenols refer to plant phenols with multiple phenolic hydroxyl groups in the molecule. Olive pomace fruit contains a high content of natural polyphenolic compounds. Plant polyphenolic substances have strong antioxidant capacity, which is 20 times that of vitamin C at the same dose, so it can effectively remove toxins from the human body. Oxygen free radicals, thereby reducing the damage of oxygen free radicals to normal cells, and playing a protective role. At the same time, polyphenolic compounds can also prevent the formation and accumulation of potential carcinogenic substances in the human body, improve metabolism, and have significant anti-cancer effects. In addition, some polyphenols with special structure in olive pomace fruit have some special effects. [0...

Claims

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Application Information

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IPC IPC(8): A61K36/32A23L33/105
CPCA61K36/32A61K2236/33A61K2236/39A61K2236/51A61K2236/53
Inventor 宋容
Owner 宋容
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