Method for preparing natural collagen film from leftover skin in fish processing
A technology of natural collagen and leftovers, applied in flexible coverings, packaging, wrapping paper, etc., can solve problems such as secondary pollution of fish skin materials, safety risks of foreign substances, etc., to increase the added value of fish skin, protect environmental effects
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Embodiment 1
[0036] After removing the meat, scales and mucus from the fresh snakeheaded fish purchased in the market, the fish skin is cut into small pieces of 0.2×0.2 cm after fully washing and draining, the ratio of material to liquid is 1:10 (w / v), and the mass concentration Stir for 5% sodium carbonate solution for 48 hours, change the solution every 12 hours, wash with water at a temperature of 20°C, use a material-liquid ratio of 1:10 (w / v), and stir for 24 hours with 10% isopropanol by volume concentration, Change the liquid every 8 hours, wash with water at 20°C, use AXAUT AP non-ionic degreasing agent with a material-to-liquid ratio of 1:10 (w / v), and a volume concentration of 3%, stir for 24 hours, change the liquid every 8 hours, and wash with water at 20°C; Then the fish skin is placed in a hydrogen peroxide solution with a material-to-liquid ratio of 1:10 (w / v), a pH of 12, and a volume concentration of 4% for 24 hours. The liquid is changed every 12 hours and cleaned with dis...
Embodiment 2
[0038] With the fresh catfish that buys on the market, after removing flesh, mucus, fully washing and draining, fish skin is cut into the fritter of 0.2 * 0.2cm, and material-liquid ratio is 1: 20 (w / v), and mass concentration is 10 % sodium bicarbonate solution was stirred for 24 hours, and the liquid was changed every 8 hours, washed with water at a temperature of 30 °C, the ratio of material to liquid was 1:20 (w / v), and the volume concentration was 15% isopropanol and stirred for 48 hours, every 12 hours Change the liquid once, wash with water at 30°C, use AXAUT-50 non-ionic degreaser with a material-to-liquid ratio of 1:20 (w / v) and a volume concentration of 5%, stir for 36 hours, change the liquid every 12 hours, and wash with water at 30°C ; then fish skin is placed in the ratio of material to liquid is 1: 20 (w / v), pH is 11, the volume concentration is 1% hydrogen peroxide solution and stirred for 12h, every 8h is changed once liquid, distilled water cleaning; The rati...
Embodiment 3
[0040] After removing the scales, meat and mucus from the fresh grass carp purchased in the market, the fish skin is cut into small pieces of 0.2×0.2 cm after fully washing and draining, the ratio of material to liquid is 1:30 (w / v), and the mass concentration Stir for 10% sodium bicarbonate solution for 36 hours, change the solution every 12 hours, wash with water at a temperature of 40°C, use a material-to-liquid ratio of 1:30 (w / v), and stir for 36 hours with isopropanol with a volume concentration of 12%. Change the liquid once every 12 hours, wash with water at 40°C, use AXAUT-50 non-ionic degreasing agent with a material-to-liquid ratio of 1:30 (w / v), and stir for 48 hours with a volume concentration of 6%, change the liquid every 12 hours, at a temperature of 40 Wash with water at ℃; then place the fish skin in a hydrogen peroxide solution with a material-to-liquid ratio of 1:30 (w / v), a pH of 12, and a volume concentration of 0.5%, stirring for 16 hours, changing the so...
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