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Method for preparing five compounds with anti-inflammation activities from oxytropis falcate bunge by separation

A technology of falciparum thorn bean and compound, which is applied in the field of separating and preparing 5 kinds of compounds with anti-inflammatory activity, can solve the problems of different content, difficulty and difficulty of high-purity compounds, etc., and achieves the effects of high product purity and large preparation amount

Active Publication Date: 2017-06-30
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, due to the large number of compounds in plants and their different contents, it is extremely difficult to directly obtain a large amount of practically valuable high-purity compounds from plants, especially when multiple target compounds need to be extracted from them at the same time.
Therefore, although it is known that Oxytropis falciparum contains a variety of compounds, there are no reports of using it as a raw material to simultaneously obtain a variety of high-purity active compounds

Method used

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  • Method for preparing five compounds with anti-inflammation activities from oxytropis falcate bunge by separation
  • Method for preparing five compounds with anti-inflammation activities from oxytropis falcate bunge by separation
  • Method for preparing five compounds with anti-inflammation activities from oxytropis falcate bunge by separation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of Oxytropis falciparum Extract

[0033] Preparation:

[0034] 1) Microwave extraction: the microwave extraction power is 731w, the extraction time is 26min, the liquid-to-solid ratio is 26, and 75% ethanol extraction.

[0035] 2) The extract is concentrated.

[0036] 3) Impurity removal by ceramic membrane filtration: 0.1um ceramic membrane, pressure 3MPa, flow rate 50ml per minute, remove fine slag, soil and other impurities, and recover the permeate solution.

[0037] 4) Separation and purification of organic membrane: Purification is carried out by using an organic membrane with a molecular weight of 2000, the pressure is 1 MPa, the flow rate is 50 ml per minute, and the permeated solution is collected.

[0038] 5) drying microwave drying, freeze drying, spray drying.

[0039] 2. Extraction:

[0040] After the Oxytropis extract extract is dissolved in deionized water 10 times its mass, it is extracted with petroleum ether and ethyl acetate in sequ...

Embodiment 2

[0046] The anti-inflammatory activity of embodiment 2 compound

[0047] For the compound chopine, 4'-hydroxy-2'-methoxychalcone, 4'-hydroxy-2'-methoxychalcone, 7-hydroxydihydroflavone, 2,4-dihydroxy-dihydrochalcone, 2,4-dihydrochalcone Hydroxy-chalcone was used for anti-inflammatory experiments, and five compounds had good anti-inflammatory activity.

[0048] 1 Cytotoxicity study

[0049] Toxicity assay of RAW264.7 cells, MTT method. The results are shown in Tables 1-5.

[0050] Table 1

[0051]

[0052] Table 2

[0053]

[0054] table 3

[0055]

[0056] Table 4

[0057]

[0058] table 5

[0059]

[0060] 2 Cultivate macrophage RAW264.7, and detect TNF-α, NO, and PEG2 indicators respectively. The results are shown in Table 6-8. For the detection methods, please refer to the following documents:

[0061] 1 Zhang Qingfang, Wang Qiangsong, Cui Yuanlu, Anti-inflammatory effect of Biqi capsule extract on RAW264.7 cell model, Chinese Patent Medicine, 2014, 36...

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Abstract

The invention discloses a method for preparing five compounds with anti-inflammation activities from oxytropis falcate bunge by separation. The five compounds include pinocembrin, 4-hydroxyl-2-methoxychalcone, 7-hydroxyflavanone, 2, 4-dihydroxyl-dihydrochalcone and 2, 4-dihydroxyl-chalcone. The method includes the steps: (1) taking total flavone extracts of the oxytropis falcate bunge, sequentially extracting by the aid of petroleum ether and ethyl acetate, and taking ethyl acetate portions; (2) taking ethyl acetate portion drying powder, purifying by the aid of a resin, eluting by the aid of methyl alcohol, drying eluting substrates to obtain purified substrates; (3) separating the purified substrates by a preparative chromatography to obtain the pinocembrin, the 4-hydroxyl-2-methoxychalcone, the 7-hydroxyflavanone, the 2, 4-dihydroxyl-dihydrochalcone and the 2, 4-dihydroxyl-chalcone. The method is large in preparation quantity, high in product purity and suitable for industrial application.

Description

technical field [0001] The invention relates to a method for separating and preparing five compounds with anti-inflammatory activity from Oxytropis falciparum. Background technique [0002] Oxytropis falcate Bunge (Tibetan medicine named Erdaxia) is a perennial herb of the genus Oxytropis D.C. in the family Leguminosae. It grows in river beaches, sandy land, hillsides, and meadows at 2700-4300m, mainly in the Qinghai-Tibet Plateau. According to "Jingzhu Materia Medica", Oxytropis falciparum has the effects of healing sores, facilitating bowel movements, preventing bone spurs, curing pain, detoxifying, and stopping bleeding. It can also detoxify, treat anthrax, leprosy, influenza, and tonsillitis. [0003] Clinical studies have proved that Oxytropis falciparum has a unique effect on the prevention and treatment of influenza and chronic bronchitis. As we all know, due to the large number of compounds in plants and their different contents, it is extremely difficult to direct...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/32C07D311/40C07C45/78C07C49/835C07C49/84A61P29/00
Inventor 陈晨李玉林刘凤芹陈涛
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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