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Microbial agent for treating coal-to-olefin wastewater

A technology of coal-to-olefins and bacterial agents, which is applied in the field of bacterial agents to treat coal-to-olefins wastewater, can solve the problems of high concentration of organic pollutants, complex chemical composition, poor biodegradability, etc., and achieve enhanced bioremediation, high treatment efficiency, Effect of reducing operating energy consumption

Pending Publication Date: 2017-06-30
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Coal chemical wastewater is an industrial wastewater that is very difficult to treat. It has a wide variety of organic pollutants with high concentrations, complex chemical composition, and poor biodegradability. It is difficult to use biological treatment methods to meet the standards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The preparation method of the bacterial agent for the treatment of coal-to-olefin wastewater comprises the following steps:

[0014] Stenotrophomonas maltophilia K279a, Arthrobacter arilaitensis Re117, Exiquobacterium sp.AT1b, Serratia liquefacines ATCC 27592, Paracoccus denitrificans PD1222) were activated and expanded. Among them, LB medium includes tryptone 10g / L, NaCl 10g / L, yeast extract 5g / L, and the rest is ionized water. The culture medium was sterilized at 121°C for 20 minutes. It should be noted that, if it is a solid medium, then 15-20 g / L of agar can be added.

[0015] Mix the Stenotrophomonas bacteria solution, Arthrobacter bacteria solution, Exiguobacterium bacteria solution, Serratia bacteria solution and Paracoccus bacteria solution after expanded culture to obtain the bacteria used for treating coal-to-olefin wastewater agent. Through plate counting, the number of live bacteria in the bacterial agent is 2.2×10 8 CFU / mL, wherein, the ratio of the nu...

Embodiment 2

[0018] The preparation method of the bacterial agent for the treatment of coal-to-olefin wastewater comprises the following steps:

[0019] Stenotrophomonas maltophilia K279a, Arthrobacter arilaitensis Re117, Exiquobacterium sp.AT1b, Serratia liquefacines ATCC 27592, Paracoccus denitrificans PD1222) were activated and expanded. Among them, LB medium includes tryptone 12g / L, NaCl 10g / L, yeast extract 4.5g / L, and the rest is ionized water. The culture medium was sterilized at 115°C for 25 minutes. It should be noted that, if it is a solid medium, then 15-20 g / L of agar can be added.

[0020] Mix the Stenotrophomonas bacteria solution, Arthrobacter bacteria solution, Exiguobacterium bacteria solution, Serratia bacteria solution and Paracoccus bacteria solution after the expanded culture to obtain the bacteria used for treating coal-to-olefin wastewater agent. Through plate counting, the number of live bacteria in the bacterial agent is 4.5×10 8 CFU / mL, wherein, the ratio of ...

Embodiment 3

[0023] The preparation method of the bacterial agent for the treatment of coal-to-olefin wastewater comprises the following steps:

[0024] Stenotrophomonas maltophilia K279a, Arthrobacter arilaitensis Re117, Exiquobacterium sp.AT1b, Serratia liquefacines ATCC 27592, Paracoccus denitrificans PD1222) were activated and expanded respectively. Among them, LB medium includes tryptone 8g / L, NaCl 11g / L, yeast extract 6g / L, and the rest is ionized water. The culture medium was sterilized at 125°C for 15 minutes. It should be noted that, if it is a solid medium, then 15-20 g / L of agar can be added.

[0025] Mix the Stenotrophomonas bacteria solution, Arthrobacter bacteria solution, Exiguobacterium bacteria solution, Serratia bacteria solution and Paracoccus bacteria solution after the expanded culture to obtain the bacteria used for treating coal-to-olefin wastewater agent. Through plate counting, the number of live bacteria in the bacterial agent is 7.5×10 8 CFU / mL, wherein, the...

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PUM

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Abstract

The invention discloses a microbial agent for treating coal-to-olefin wastewater. The microbial agent contains stenotrophomonas maltophilia K279a, arthrobacter arilaitensis Re117, exiquobacterium sp.AT1b, serratia liquefacines ATCC 27592 and paracoccus denitrificans PD1222. The microbial agent provided by the invention can improve COD and NH3-N removal ability of coal chemical engineering wastewater which is mainly caused by adopting coal-to-olefin as a main process of biochemical engineering treatment, and the treatment effect is stable. The microbial agent is added into a styrene-butadiene rubber (SBR) reactor, so that the COD and NH3-N removal efficiency reaches 99% and is stably maintained at the level.

Description

technical field [0001] The invention relates to the technical field of coal chemical wastewater treatment, in particular to a bacterial agent for treating coal-to-olefin wastewater. Background technique [0002] Coal chemical wastewater is an industrial wastewater that is very difficult to treat. It has a wide variety of organic pollutants with high concentrations, complex chemical composition, and poor biodegradability. It is difficult to use biological treatment methods to meet the standards. In addition to a large number of solid suspended particles and fused-ring aromatic hydrocarbons, volatile phenolic compounds, naphthalene, pyrrole, furan, oil and other toxic and harmful substances and refractory organic substances, wastewater also contains many inorganic pollutants, such as ammonia nitrogen, sulfide, etc., COD value and high chroma. Coal-to-olefin wastewater belongs to coal chemical industry wastewater and has the above properties. Contents of the invention [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/06C12R1/425C02F103/34
CPCC02F3/34C02F3/348C02F2103/34C12N1/20
Inventor 孙寓姣王蕾郑丹阳
Owner BEIJING NORMAL UNIVERSITY
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