Fermentation method and application of high-concentration clostridium butyricum

A technology of Clostridium butyricum and fermentation method, which is applied in the field of fermentation of high-concentration Clostridium butyricum, can solve the problems that the value of fermentation products cannot be fully utilized, the concentration of bacteria is not high, and the synthesis of metabolite butyric acid is low. Improve industrialization advantages, increase the concentration of bacteria, and promote the effect of synthesis

Inactive Publication Date: 2017-07-07
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the technical problems to be solved by the present invention is to provide a high-concentration Clostridium butyricum fermentation method to solve the problems of low bacterial concentration and low synthesis of metabolite butyric acid in the existing technology
[0005] The second technical problem to be solved by the present invention is to provide an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1), biological fermentation of high concentration of Clostridium butyricum

[0031] 1.1 Primary seed preparation: prepare seed medium, sterilize and cool to room temperature, inoculate the butyric acid bacteria strain tube into the seed medium at a volume fraction (V / V) of 0.1%, and cultivate at 35°C for 30 hours to obtain first-class seed liquid;

[0032] 1.2 Secondary seed preparation: prepare seed medium, sterilize and cool to room temperature, inoculate the primary seed liquid into the seed medium at a volume fraction (V / V) of 0.2%, and culture at 35°C for 20 hours to prepare secondary seeds liquid;

[0033] 1.3 Preparation of fermentation medium: prepare fermentation medium, sterilize and cool to room temperature;

[0034] 1.4 Inoculation and fermentation: Inoculate the Clostridium butyricum seed liquid into the Clostridium butyricum fermentation medium according to the volume fraction (V / V) of 5%, and cultivate it at pH 6.0 and temperature 35°C for 20 hours;

...

Embodiment 2

[0045] 1), biological fermentation of high concentration of Clostridium butyricum

[0046] 1.1 Primary seed preparation: prepare seed medium, sterilize and cool to room temperature, inoculate the butyric acid bacteria strain tube into the seed medium at a volume fraction (V / V) of 1%, and cultivate at 38°C for 20 hours to obtain first-class seed liquid;

[0047] 1.2 Secondary seed preparation: prepare seed medium, sterilize and cool to room temperature, inoculate the primary seed liquid into the seed medium according to the volume fraction (V / V) of 2%, and cultivate at 38°C for 10 hours to prepare secondary seeds liquid;

[0048] 1.3 Preparation of fermentation medium: prepare fermentation medium, sterilize and cool to room temperature;

[0049] 1.4 Inoculation and fermentation: Inoculate the Clostridium butyricum seed liquid into the Clostridium butyricum fermentation medium according to the volume fraction (V / V) of 10%, and cultivate it at pH 6.5 and temperature 38°C for 10...

Embodiment 3

[0060] 1), biological fermentation of high concentration of Clostridium butyricum

[0061] 1.1 First-level seed preparation: prepare seed medium, cool to room temperature after sterilization, inoculate the butyric acid bacteria strain tube into the seed medium at a volume fraction (V / V) of 0.6%, and cultivate at 37°C for 25 hours to obtain first-class seed liquid;

[0062] 1.2 Secondary seed preparation: prepare seed medium, sterilize and cool to room temperature, inoculate the primary seed liquid into the seed medium at a volume fraction (V / V) of 1%, and culture at 37°C for 15 hours to obtain secondary seeds liquid;

[0063] 1.3 Preparation of fermentation medium: prepare fermentation medium, sterilize and cool to room temperature;

[0064] 1.4 Inoculation and fermentation: Inoculate the Clostridium butyricum seed liquid into the Clostridium butyricum fermentation medium according to the volume fraction (V / V) of 8%, and cultivate for 15 hours at pH 6.3 and temperature 37°C;...

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Abstract

The invention relates to a fermentation method and application of high-concentration clostridium butyricum. The fermentation method includes the following steps that S100, inoculated fermentation, wherein clostridium butyricum strain liquid with the volume fraction of 5-10% is inoculated to a clostridium butyricum fermentation culture medium and cultured at the temperature of 35-38 DEG C and pH of 6.0-6.5 for 10-20 hours; S200, fed-batch fermentation, wherein fed batch of a mixed carbon source is conducted, the fed-batch fermentation is conducted for 20-40 hours, and the mixed carbon source includes 60-100 g/L sugar and 100-140 g/L sodium acetate; S300, sporulating fermentation, wherein after fed-batch fermentation is completed, a compound liquid containing calcium, magnesium and manganese is added, the sporulating fermentation is conducted for 10-30 hours, and clostridium butyricum is sporulated. The fermentation method largely improves the concentration of clostridium butyricum thalli, promotes the synthesis of butyric acid as a metabolite and can also produce clostridium butyricum and butyric acid with the high performance-price ratio, the advantages of industrialization are further improved, benefits are increased, and the environmental-protection requirements are achieved and met.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a fermentation method and application of high-concentration Clostridium butyricum. Background technique [0002] Intestinal microbiota has become the focus of academic and production applications at home and abroad. As a microecological agent that regulates the balance of intestinal microbiota and replaces the use of antibiotics, its application in animal intestinal health has attracted more and more attention from the whole society. However, subject to microbial research methods and production technologies, the industry mainly produces aerobic bacteria and facultative anaerobic bacteria. More than 99% of animal intestinal microbiota are strictly anaerobic bacteria, and through intestinal metagenomic research, it is found that symbiotic Clostridium is the main anaerobic bacteria. Therefore, the research and development direction and trend of animal microecologic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/52C12R1/145
CPCC12N1/20C12P7/52
Inventor 凌华云詹志春周樱张东晓邱权
Owner 湖北华扬科技发展有限公司
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