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Rapid high-efficiency tissue culture method for garlic

A tissue culture and garlic technology, applied in the field of fast and efficient garlic tissue culture, can solve the problems of long in vitro culture time, low reproduction coefficient, high cost of rapid propagation, etc., and achieve the goals of shortening culture time, high reproduction coefficient and reducing pollution rate Effect

Active Publication Date: 2017-07-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a fast and efficient garlic tissue culture method to solve the problems of serious vitrification, low reproduction coefficient, long in vitro culture time and high cost of rapid propagation in the prior art.

Method used

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  • Rapid high-efficiency tissue culture method for garlic
  • Rapid high-efficiency tissue culture method for garlic
  • Rapid high-efficiency tissue culture method for garlic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The test material is the inflorescence rachis of "Ershuizao" grown naturally in the field, 1 day, 5 days, 10 days and 15 days after natural bolting. After measuring the relative length of the inflorescence rachis, select the inflorescence rachis with the same diameter of the flower stem, and cut off the involucral band About 1cm stalks, completely submerged in saturated washing powder solution for about 30 minutes, rinsed with running water for about 30 minutes to sterilize the surface, put the fully washed materials on the ultra-clean workbench, and sterilized the surface with 75% alcohol for 60 seconds. Rinse once with sterile water, then treat with 2% sodium hypochlorite solution for 12 minutes, rinse with sterile water 4 to 5 times, and finally place on sterile filter paper to blot the surface moisture.

[0047] Peel off the outer bracts of the rachis, remove all the degenerated flower primordium residues and membranous bracts on the rachis surface, remove the part o...

Embodiment 2

[0058] With reference to the method described in Example 1, the test material was grown naturally in the field. Five days after the natural bolting, the inflorescence rachis of "Er Shui Zao" was cut off about 1 cm of the involucre belt, and completely immersed in a saturated washing powder solution for about 30 minutes. , wash the surface with running water for about 30 minutes to sterilize, put the fully washed materials on the ultra-clean workbench, and set up 9 treatments:

[0059] T1: 75% alcohol treatment for 30 seconds, 2% sodium hypochlorite solution for 10 minutes;

[0060] T2: 75% alcohol treatment for 30 seconds, 2% sodium hypochlorite solution for 15 minutes;

[0061] T3: 75% alcohol treatment for 30 seconds, 2% sodium hypochlorite solution for 20 minutes;

[0062] T4: 75% alcohol treatment for 60 seconds, 2% sodium hypochlorite solution for 10 minutes;

[0063] T5: 75% alcohol treatment for 60 seconds, 2% sodium hypochlorite solution for 15 minutes;

[0064] T6:...

Embodiment 3

[0076] Referring to the method described in Example 1, the test material was grown naturally in the field, and the inflorescence rachis of "Eshuizao" 5 days after natural bolting was treated with 75% alcohol for 60 seconds, and after 15 minutes with 2% sodium hypochlorite, set 0.05%, 0.10% , 0.20, 0.40% hydrogen peroxide treatment for 5 minutes, other processing and statistical methods are the same as experimental case 1:

[0077] Table 3 Effects of different concentrations of hydrogen peroxide on contamination rate, reproduction coefficient and vitrification rate of adventitious buds of garlic

[0078]

[0079]Result shows: adopt scheme described in the present invention, adopt the hydrogen peroxide of 0.1~0.2% concentration to further prevent pollution, and obviously reduce vitrification rate and improve reproduction coefficient, when not only pollution rate can rise sharply when lower than this concentration, And the vitrification rate will increase significantly. If it ...

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Abstract

The invention discloses a rapid high-efficiency tissue culture method for garlic. On the basis of guaranteeing of a high propagation coefficient, the rapid high-efficiency tissue culture method can reduce the contamination rate and variation rate of garlic in the process of tissue culture, overcome the problems of high contamination rate and frequent vitrification in the process of tissue culture of test-tube garlic plantlets in the prior art, obtain a great number of normal test-tube garlic plantlets with consistent sizes in a short period of time and reduce tissue culture cost for garlic; and the rapid high-efficiency tissue culture method can be applied to industrialized production and scientific research in a large scale.

Description

technical field [0001] The invention relates to plant tissue culture technology, and specifically designs a fast and efficient garlic tissue culture method. Background technique [0002] Garlic (Allium sativum L.), also known as garlic and gourd garlic, is a biennial herb of the Liliaceae Allium genus. It is known as "plant gold" and is a vegetable and medicine that is popular worldwide and has important edible and medicinal values. It is also an important export vegetable that China has strong competitiveness in the international market, and its intensively processed products are popular in Europe and North America. Due to the difficulty in flowering and fruiting of garlic, bulbs are used for vegetative propagation, which not only requires a large amount of seeds and a low reproduction coefficient, but also, as a perennial vegetative crop, is prone to the accumulation of bacteria, fungi, and viruses, resulting in the degradation of the species, and the decline in yield and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴震刘敏蒋芳玲田洁孔祥宇程雅琪
Owner NANJING AGRICULTURAL UNIVERSITY