Preparation method for gelatin/hydroxyapatite composite coating

A hydroxyapatite and composite coating technology, applied in coatings, pharmaceutical formulations, pharmaceutical science and other directions, can solve problems such as poor affinity, and achieve the effect of improving poor affinity and increasing interface compatibility

Inactive Publication Date: 2017-07-21
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a gelatin / hydroxyapatite composite coating on the surface of bone tissue engineering scaffolds for the disadvantage of poor affinity of existing artificially synthesized bone scaffold materials, so as to realize the improvement of bone scaffold materials. modified

Method used

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  • Preparation method for gelatin/hydroxyapatite composite coating
  • Preparation method for gelatin/hydroxyapatite composite coating
  • Preparation method for gelatin/hydroxyapatite composite coating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Add 1 g of gelatin particles (about 57306 molecular weight) into deionized water to swell for 2 hours, and then heat to 50°C to form a 2wt% gelatin solution. After the gelatin is completely dissolved, add 25mL of 0.2M CaCl 2 Add the solution into the gelatin solution, and slowly add 0.12M NaH 2 PO 4 The solution was stirred at 50° C. for 4 h after the dropwise addition, and aged for 1 h to obtain a mixed gelatin solution.

[0041] Soak the PLLA / β-TCP scaffold sheet cut into about 10mm*10mm in 20mL ethanol:water (v / v)=1:1 for 2h, then rinse with plenty of water, soak the cleaned sheet in 1,6 -Hexamethylenediamine / isopropanol=2.4g / 20mL, react at 45°C for 10min, deionize and wash for 12h, and vacuum dry for 24h to obtain aminated flakes, which are then soaked in 10mL of 1wt% 1,5-pentanediene Aldehyde solution, deionized cleaning 12h, vacuum drying to obtain flakes.

[0042] Prepare a mixed gelatin solution of 3 mg / mL, soak the above sheet in the mixed gelatin solution f...

Embodiment 2

[0049] Add 1 g of gelatin particles (about 49317 molecular weight) into deionized water to swell for 2 hours, and then heat to 50°C to form a 2wt% gelatin solution. After the gelatin is completely dissolved, add 25mL of 0.2M CaCl 2 Add the solution into the gelatin solution, and slowly add 0.12M NaH 2 PO 4 The solution was stirred at 50° C. for 4 h after the dropwise addition, and aged for 1 h to obtain a mixed gelatin solution.

[0050] Soak the PLLA / β-TCP scaffold sheet cut into about 10mm*10mm in 20mL ethanol:water (v / v)=1:1 for 2h, then rinse with plenty of water, soak the cleaned sheet in 1,6 -Hexamethylenediamine / isopropanol=2.4g / 20mL, react at 45°C for 10min, deionize and wash for 12h, and vacuum dry for 24h to obtain aminated flakes, which are then soaked in 10mL of 1wt% 1,5-pentanediene Aldehyde solution, deionized cleaning 12h, vacuum drying to obtain flakes.

[0051] The surface contact angle of PLLA / β-TCP scaffold decreased from 82.2° before modification to 50.4...

Embodiment 3

[0054] Add 1 g of gelatin particles (about 38379 molecular weight) into deionized water to swell for 2 hours, then heat to 50°C to form a 2wt% gelatin solution. After the gelatin is completely dissolved, add 25 mL of 0.2M CaCl 2 Add the solution into the gelatin solution, and slowly add 0.12M NaH 2 PO 4 The solution was stirred at 50° C. for 4 h after the dropwise addition, and aged for 1 h to obtain a mixed gelatin solution.

[0055] Soak the PLLA / β-TCP scaffold sheet cut into about 10mm*10mm in 20mL ethanol:water (v / v)=1:1 for 2h, then rinse with plenty of water, soak the cleaned sheet in 1,6 -Hexamethylenediamine / isopropanol=2.4g / 20mL, react at 45°C for 10min, deionize and wash for 12h, and vacuum dry for 24h to obtain aminated flakes, which are then soaked in 10mL of 1wt% 1,5-pentanediene Aldehyde solution, deionized cleaning 12h, vacuum drying to obtain flakes.

[0056] The surface contact angle of PLLA / β-TCP scaffold decreased from 82.2° before modification to 51.8° a...

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Abstract

The invention specifically relates to a preparation method for a gelatin/hydroxyapatite composite coating used for the surface of a bone tissue engineering scaffold. According to the invention, the composite coating is prepared through in-situ synthesis with a novel bone tissue engineering scaffold as a matrix material and gelatin, a calcium salt and phosphate as main raw materials; a calcium salt solution and a phosphate solution are added into a gelatin solution, and a composite coating solution is synthesized under the condition of heating; then the surface of the scaffold is subjected to amination and aldehydation modification; the gelatin/hydroxyapatite composite coating is finally loaded onto the surface of the scaffold; and since the surface of the scaffold is loaded with gelatin and nanometer hydroxyapatite, the defect of poor affinity between artificial synthetic high polymer materials and bone tissue is overcome, and the prepared scaffold can meet requirements of clinical medicine on cell affinity, biocompatibility and the like.

Description

technical field [0001] The invention belongs to the technical field of biological coating materials, and in particular relates to a preparation method of a gelatin / hydroxyapatite composite coating used on the surface of a bone tissue engineering support. Background technique [0002] With the development of tissue engineering, more and more biomaterials have been applied in the field of tissue engineering to repair damaged tissues and realize the function of tissue regeneration. The current tissue engineering materials are mainly natural polymer materials, synthetic polymer materials and bioactive ceramics. Among them, bone tissue engineering scaffold material is one of the research hotspots in the field of biomaterials. As a tissue engineering carrier material for bone tissue repair and reconstruction, it is required to have good biocompatibility, controllable biodegradability, appropriate three-dimensional structure of connected pores, certain mechanical strength and good...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/32A61L27/18A61L27/12
CPCA61L27/12A61L27/18A61L27/32A61L27/34A61L2400/18A61L2420/02A61L2420/04A61L2430/02C08L89/00C08L67/04
Inventor 范仲勇杜焙焙王杰林
Owner FUDAN UNIV
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