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Schisandra chinensis polysaccharide extract as well as preparation method and application thereof

An extract of Schisandra chinensis and technology of Schisandra chinensis, which are applied in medical formulas, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of lack of prevention and control methods and restrict the healthy development of cold-water fish farming industry

Active Publication Date: 2017-07-21
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of effective control methods, IHN has become a bottleneck problem restricting the healthy development of China's cold-water fish farming industry

Method used

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  • Schisandra chinensis polysaccharide extract as well as preparation method and application thereof
  • Schisandra chinensis polysaccharide extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、 5

[0058] Embodiment 1, the preparation of Schisandra polysaccharide extract

[0059] 1. Dry Schisandra chinensis in a constant temperature box at 60°C until constant weight, then pulverize and pass through a 20-mesh sieve to obtain dry powder of Schisandra chinensis.

[0060] 2. Add the schisandra dry powder obtained in step 1 into distilled water, soak at room temperature for 2 hours (25ml distilled water per 1g of schisandra dry powder), and then perform ultrasonic treatment (place the whole system in an ultrasonic oscillator and use 360W power for ultrasonic treatment for 30min), then Place in a constant temperature water bath for high-temperature leaching (75°C, 3h), then centrifuge at 4000rpm for 10min, and collect the supernatant and precipitate respectively.

[0061] 3. Add the precipitate obtained in step 2 into distilled water (10ml distilled water per 1g wet weight of the precipitate), then carry out high-temperature leaching in a constant temperature water bath (75°C,...

Embodiment 2、 5

[0074] Example 2, Detection of Cytotoxicity of Schisandra Polysaccharide Extract

[0075] The concentration of Schisandra polysaccharides in this example is calculated based on the proportion of Schisandra polysaccharides in the Schisandra polysaccharide extract in Step 10 of Example 1.

[0076] 1. Get the schisandra polysaccharide extract prepared in Example 1 and the M199 medium (containing 2% FBS, 100U / ml penicillin and 100U / ml streptomycin), and prepare the following working solution:

[0077] Working solution A (normal cell control group): M199 medium (containing 2% FBS, 100 U / ml penicillin and 100 U / ml streptomycin).

[0078] Working solution B (experimental group I): Dissolve the extract of Schisandra polysaccharide in M199 medium (containing 2% FBS, 100U / ml penicillin and 100U / ml streptomycin) to obtain working solution B; Schisandra polysaccharide in working solution B The concentration was 25 μg / mL.

[0079] Working solution C (experimental group II): Dissolve the ...

Embodiment 3、 5

[0087] Example 3, Study on Anti-IHNV Activity of Schisandra Polysaccharide Extract

[0088] The concentration of Schisandra polysaccharides in this example is calculated based on the proportion of Schisandra polysaccharides in the Schisandra polysaccharide extract in Step 10 of Example 1.

[0089] 1. Get the schisandra polysaccharide extract prepared in Example 1 and the M199 medium (containing 2% FBS, 100U / ml penicillin and 100U / ml streptomycin), and prepare the following working solution:

[0090] Working solution A (treatment group I): the Schisandra polysaccharide extract was dissolved in M199 medium (2% FBS, 100U / ml penicillin and 100U / ml streptomycin) to obtain working solution A; The concentration was 3.12 μg / mL.

[0091] Working solution B (treatment group II): the Schisandra polysaccharide extract was dissolved in M199 medium (2% FBS, 100U / ml penicillin and 100U / ml streptomycin) to obtain working solution B; The concentration was 6.25 μg / mL.

[0092] Working soluti...

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Abstract

The invention discloses a schisandra chinensis polysaccharide extract as well as a preparation method and application thereof. The preparation method of the schisandra chinensis polysaccharide extract sequentially comprises the following steps: (a1) taking schisandra chinensis, performing water extraction and collecting liquid phase, wherein ultrasonic pretreatment is conducted before water extraction; (a2) removing protein and micromolecular impurities and performing alcohol precipitation to obtain the schisandra chinensis extract. The invention also protects the schisandra chinensis extract prepared by the method. The invention also protects application of the schisandra chinensis extract to inhibition of IHNV (infectious hematopoietic necrosis virus). The invention provides certain theoretical basis for screening novel anti-IHNV medicines.

Description

technical field [0001] The invention relates to a schisandra polysaccharide extract, a preparation method and application thereof. Background technique [0002] Infectious hematopoietic necrosis (IHN) is the most deadly viral disease affecting the development of salmon and trout industry in my country. Its pathogen is infectious hematopoietic necrosis virus (IHNV). IHNV belongs to the Novirhabdovirus genus of the Rhabdoviridae family and mainly infects salmon and trout, including rainbow trout, salmon, Atlantic salmon, and steelhead salmon. Once the virus invades the fish body, it first proliferates in the hematopoietic tissues of the fish body kidney and spleen, and after entering the blood circulation, it will multiply in various organs of the fish body, causing sudden death of salmon and trout. Depending on fish species, age, virus strain, and environmental conditions, outbreaks of IHN can result in mortality approaching 100% in salmon trout fry and 25-30% in adult fish....

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P31/12
CPCC08B37/0003
Inventor 任广明徐黎明赵景壮卢彤岩尹家胜
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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