Method for preparing chimeric antigen receptor T cells

A chimeric antigen receptor and cell technology, applied in the field of biomedicine, can solve the problems of low lentivirus titer, low efficiency, and hinder the wide clinical application of T cells, and achieve the effect of increasing activation ability and improving infection efficiency.
CN106978442AActive Publication Date: 2017-07-25ICARTAB BIOMEDICAL
5 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ICARTAB BIOMEDICAL
Publication Date
2017-07-25

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for preparing chimeric antigen receptor T cells. The method comprises the steps of mixing a chimeric antigen receptor slow virus expression vector, pGP plasmids, pVSVG plasmids and polyetherimide firstly, and conducting uniform blow-beating to obtain a DNA / PEI compound; inoculating a culture dish with 293 cells, and then adding a complete medium for culture; adding the DNA / PEI compound to the culture dish dropwise; removing the medium after 4-10 h of culture; adding a conditioned medium, continuing culture for 8-32 h, and then collecting the medium; conducting centrifugal treatment to obtain medium supernatant containing slow viruses; adding a sucrose solution into a centrifugal tube, and then adding the medium supernatant containing the slow viruses along the wall of the centrifugal tube; conducting centrifugal treatment, removing centrifugation supernatant, and then adding a buffer solution; and then conducting T cell transfection to obtain the chimeric antigen receptor T cells through culture. Based on a slow virus packaging system, transfection efficiency can reach 60% or more.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of chimeric antigen receptor T cells, which can obviously improve the transfection efficiency of carriers to T cells. Background technique

[0002] T lymphocytes play a major role in the tumor immune response and have a strong killing effect on tumor cells. However, there are MHC limitations when using endogenous T cells for tumor immunotherapy. The process of tumor immunoediting will reduce the expression of MHC on the surface of tumor cells, destroy the antigen processing process, reduce the immunogenicity of peptides, and form an immune escape mechanism. The T cell therapy technology that enables tumor cells to successfully evade T cell attacks and rapidly proliferate by using genetic modification technology to express tumor-specific chimeric antigen receptors has shown good targeting, killing activity and persistence in vitro and clinical ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More