A kind of preparation method and application of selenium-enriched Moringa feed additive
A technology of feed additive and Moringa oleifera, which is applied in the field of preparation of selenium-enriched moringa oleifera feed additive, can solve the problems of not fully considering the absorption efficiency of selenium element and the demand of other elements, so as to shorten the process time, simplify the process steps and ensure the stability Effect
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Embodiment 1
[0025] 1. Preliminary preparation
[0026] The nutrient solution includes the following raw materials: NH 4 CL 1g / L, MgCL 2 ·6H 2 O 0.13g / L, CaCL 2 ·2H 2 O 0.1g / L, KH 2 PO 4 0.5g / L, NaCL0.1g / L, trace element solution 10g / L, vitamin solution 10g / L, yeast powder 1.5g / L, 1.2g / L peptone 1.2g / L, morpholine ethanesulfonic acid 5.0g / L , Cysteine hydrochloride 0.5g / L, sucrose 2.5g / L, sucrose 3.0g / L, pH 6.5.
[0027] Preparation of active liquid: In parts by weight, 10 parts of milk, 0.3 parts of lactic acid bacteria seed liquid, 5 parts of distiller’s grains and 0.5 part of gram-positive actinomycete seed liquid are dissolved in 300 parts of nutrient solution and activated at 20°C 6h, then add 12 parts of sodium alginate, while stirring and reacting at a speed of 1500r / min for 30min, the active liquid is obtained.
[0028] The above-mentioned material prepared in the early stage is used in the preparation method of the following selenium-enriched Moringa feed additive.
[0029] 2. A prepar...
Embodiment 2
[0035] 1. Preliminary preparation
[0036] The nutrient solution includes the following raw materials: NH 4 CL 1g / L, MgCL 2 ·6H 2 O 0.13g / L, CaCL 2 ·2H 2 O 0.1g / L, KH 2 PO 4 0.5g / L, NaCL0.1g / L, trace element solution 10g / L, vitamin solution 10g / L, yeast powder 1.5g / L, 1.2g / L peptone 1.2g / L, morpholine ethanesulfonic acid 5.0g / L , Cysteine hydrochloride 0.5g / L, sucrose 2.5g / L, sucrose 3.0g / L, pH 6.5.
[0037] Preparation of active liquid: In parts by weight, 10 parts of milk, 0.5 part of lactic acid bacteria seed liquid, 6 parts of distiller’s grains and 0.7 part of gram-positive actinomycete seed liquid are dissolved in 330 parts of nutrient solution and activated at 22°C After 7 hours, 20 parts of sodium alginate were added, and the reaction was stirred at a speed of 2000 r / min for 33 minutes to obtain the active liquid.
[0038] The above-mentioned material prepared in the early stage is used in the preparation method of the following selenium-enriched Moringa feed additive.
[0...
Embodiment 3
[0045] 1. Preliminary preparation
[0046] The nutrient solution includes the following raw materials: NH 4 CL 1g / L, MgCL 2 ·6H 2 O 0.13g / L, CaCL 2 ·2H 2 O 0.1g / L, KH 2 PO 4 0.5g / L, NaCL0.1g / L, trace element solution 10g / L, vitamin solution 10g / L, yeast powder 1.5g / L, 1.2g / L peptone 1.2g / L, morpholine ethanesulfonic acid 5.0g / L , Cysteine hydrochloride 0.5g / L, sucrose 2.5g / L, sucrose 3.0g / L, pH 6.5.
[0047] Preparation of active liquid: In parts by weight, 10 parts of milk, 0.6 parts of lactic acid bacteria seed liquid, 8 parts of distiller’s grains and 0.9 parts of gram-positive actinomycete seed liquid are dissolved in 400 parts of nutrient solution and activated at 25°C 8h, then add 22 parts of sodium alginate, while stirring and reacting at a speed of 2200r / min for 41min, the active liquid is obtained.
[0048] The above-mentioned material prepared in the early stage is used in the preparation method of the following selenium-enriched Moringa feed additive.
[0049] 2. A prepa...
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