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Separating and purifying method of Arctic marine rhodococcus B7740 produced isoprenoid

A technology for separation and purification of isoprenoids, which is applied in the field of separation and purification of isoprenoids produced by Rhodococcus B7740, can solve the problems of different types of structures, large differences in structures, and increasing the difficulty of purification.

Active Publication Date: 2017-08-08
HUAZHONG AGRI UNIV +1
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of carotenoids by Rhodococcus B7740 is due to the unique metabolic pathway of this microorganism. Compared with carotenoids from common higher plants, the structure is quite different, and the structure types are not single. Its unique structure not only endows it with unique activity, but also increases the difficulty of purifying

Method used

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  • Separating and purifying method of Arctic marine rhodococcus B7740 produced isoprenoid
  • Separating and purifying method of Arctic marine rhodococcus B7740 produced isoprenoid
  • Separating and purifying method of Arctic marine rhodococcus B7740 produced isoprenoid

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Embodiment 1

[0031] Isolation and Purification of Isoprenoids Produced by Arctic Marine Rhodococcus B7740

[0032] 1. Experimental materials

[0033] 1.1. Experimental reagents

[0034] Lysozyme (20000U / mg) was purchased from Sinopharm Chemical Reagent Co., Ltd., zinc acetate, sodium chloride, methanol and methylene chloride were purchased from Sinopharm Chemical Reagent Co., Ltd., n-hexane and dichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd. Company; chromatographic grade methanol and acetonitrile were purchased from Thermo Fisher Scientific (China) Co., Ltd.; chromatographic grade methyl tert-butyl ether was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0035] 1.2. Experimental Instruments

[0036] TBE-300C high-speed countercurrent chromatograph was purchased from Shanghai Tongtian Biotechnology Co., Ltd.; 2695 high-performance liquid chromatograph was purchased from Waters, USA; LTQ-Orbitrap Elite mass spectrometer was purchased from Therm...

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Abstract

The invention discloses a separating and purifying method of Arctic marine rhodococcus B7740 produced isoprenoid. The method utilizes high-speed counter-current chromatography to separate and purify the Arctic marine rhodococcus B7740 produced isoprenoid. The method has the advantages that the isoprenoid produced by the Arctic marine rhodococcus B7740 is effectively separated and purified by the high-speed counter-current chromatography, so as to obtain three types of rare marine-derived carotenoids; the three types of rare marine-derived carotenoids are further identified by high-precision mass spectrometry.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of isoprenoids produced by Rhodococcus B7740, and in particular relates to a method for separating and purifying isoprenoids produced by Rhodococcus B7740 in the Arctic Ocean. Background technique [0002] Rhodococcus is a kind of Gram-positive bacteria widely distributed in nature. It is abundant in soil, seabed sediment and herbivore feces, and most of them are saprophytic. The genus Rhodococcus (Rhodococcus sp.) was established by Zoof in 1891. Since its establishment, its taxonomic status has been uncertain. Currently, Rhodococcus belongs to Actinobacteria, Actinobacteria, Actinobacteridae, Actinomycetales, Croynebacterineae, Nocardia Nocardiaceae, Rhodococcus. [0003] At present, there are many studies on Rhodococcus erythropolis at home and abroad, mainly focusing on its biodegradability research and molecular research, but there are few studies on the isoprenoid subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 孙智达陈亚淑杨季芳陈吉刚谢笔钧
Owner HUAZHONG AGRI UNIV
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