A solution for improving the detection of human testicular sperm
A solution and testicular technology, applied in microorganisms, culture process, animal cells, etc., can solve problems such as difficulty in distinguishing vitality, testicular sperm immobility, etc., and achieve the effect of improving antioxidant capacity, increasing activity, and increasing ratio.
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Embodiment 2
[0029] First prepare the basic solution, the components of the basic solution and their final concentrations are as follows: sodium chloride 110mmol / L, potassium chloride 3.5mmol / L, magnesium sulfate 0.8mmol / L, calcium chloride 5mmol / L, sodium dihydrogen phosphate 0.1mmol / L, sodium lactate 10mmol / L, sodium bicarbonate 35mmol / L. After the dissolution of the above components is completed, adjust the pH of the solution to 7.2 with sodium hydroxide and hydrochloric acid solution configured in advance, and then add 4-hydroxyethylpiperazineethanesulfonic acid buffer to the solution. The final concentration of the buffer is 10mmol / L. Then add the weighed sodium pyruvate, caffeine and pentoxifylline to the solution successively, and their final concentrations are respectively sodium pyruvate 0.5mmol / L, caffeine 5mmol / L, and pentoxifylline 10mmol / L. After the dissolution was completed, 1%wt of phenol red was added to the solution, and the solution was filtered and sterilized with a s...
Embodiment 3
[0034]First prepare the basic solution, the components of the basic solution and their final concentrations are as follows: sodium chloride 100mmol / L, potassium chloride 3.2mmol / L, magnesium sulfate 0.5mmol / L, calcium chloride 2.0mmol / L, dihydrogen phosphate Sodium 0.053mmol / L, sodium lactate 10mmol / L, sodium bicarbonate 30.9mmol / L. After the dissolution of the above components is completed, adjust the pH of the solution to 7.2 with sodium hydroxide and hydrochloric acid solution configured in advance, and then add 4-hydroxyethylpiperazineethanesulfonic acid buffer to the solution. The final concentration of the buffer is 8mmol / L. Then add the weighed sodium pyruvate, caffeine and pentoxifylline successively in the solution, and their final concentrations are respectively sodium pyruvate 0.25mmol / L, caffeine 2.5mmol / L, and pentoxifylline 7.5mmol / L. After the dissolution was completed, 1%wt of phenol red was added to the solution, and the solution was filtered and sterilized ...
Embodiment 7
[0054] First prepare the basic solution, the components of the basic solution and their final concentrations are as follows: sodium chloride 100mmol / L, potassium chloride 3.2mmol / L, magnesium sulfate 0.5mmol / L, calcium chloride 2.0mmol / L, dihydrogen phosphate Sodium 0.053mmol / L, sodium lactate 10mmol / L, sodium bicarbonate 30.9mmol / L. After the dissolution of the above components is completed, adjust the pH of the solution to 7.4 with sodium hydroxide and hydrochloric acid solution configured in advance, and then add 4-hydroxyethylpiperazineethanesulfonic acid buffer to the solution. The final concentration of the buffer is 10mmol / L. Then add the weighed sodium pyruvate and pentoxifylline to the solution successively, and their final concentrations are respectively sodium pyruvate 0.25mmol / L and pentoxifylline 7.5mmol / L. After the dissolution was completed, 1%wt phenol red was added to the solution, and then the solution was filtered and sterilized under the ultra-clean bench...
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