Preparation method of microbial preparation for treating mikania micrantha kunth

A microbial preparation, the technology of Mikania micrantha, applied in the direction of chemicals for biological control, botanical equipment and methods, biocides, etc., can solve problems such as easy harm to other plants, slow effect of chemical agents, and other plant hazards. , to achieve the effect of easy promotion, convenient and simple promotion, and reduction of prevention and control costs

Inactive Publication Date: 2017-08-18
雷笑天
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the problems of slow effect of chemical agents, high cost, low control efficiency and easy harm to other plants, the present invention uses cow dung, biogas slurry, and discarded mushroom sticks as bacterial sources, and peat coal As a nutrient, expand the population by fermentation, use N-methyl-N-nitrosourea as an inducer, and use the squeezed juice of fresh Mikania micrantha stem as a screening nutrient to obtain the target mixed bacteria, and then through enrichment culture to obtain the following Fresh Mikania micrantha stems are used as nutrient strains, and then calcined and soaked vermiculite in glucose is used as a carrier to absorb microorganisms, and sodium alginate is used to wrap them to obtain microbial preparations for treating Mikania micrantha. The microbial preparations prepared in the present invention The microorganisms in it use the stems of Mikania micrantha as nutrients, cut off the asexual reproduction of Mikania micrantha and the production of seeds, and the effect of treating Mikania micrantha is good, effectively solving the problems of slow chemical agent effect, high cost, low control efficiency and easy harm to other plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] In terms of parts by weight, take 50 parts of cow dung, 40 parts of peat, 14 parts of biogas slurry and 6 parts of discarded mushroom sticks, put them into the mixer, stir at 180r / min for 30min, and then spread the stirred mixture on the cement In the pool, spread the thickness of 8cm, use straw to cover the mixture, cover the thickness of 2m, and cover for 2 days; according to the mass ratio of 1:50:200, take N-methyl-N-nitrosourea, fresh Mikania micrantha stems and mass Put the glucose solution with a fraction of 2% into a juice extractor to squeeze the juice, and collect the squeezed juice; after the above-mentioned straw-covered mixture is finished, remove the straw, let it ventilate naturally for 13 days, and stir at 120r / min every 32 hours during the period of natural ventilation Remove the mixture after removing the straw for 10 minutes, and add juice equal to the biogas slurry in the mixture to the mixture after removing the straw every 4 days; after the above-me...

example 2

[0021]In terms of parts by weight, take 60 parts of cow dung, 50 parts of peat, 16 parts of biogas slurry and 9 parts of discarded mushroom sticks, put them into a mixer, stir at 180r / min for 40min, and spread the stirred mixture on the cement In the pool, the spreading thickness is 13cm, the mixture is covered with straw, the covering thickness is 4cm, covering for 3 days; by mass ratio 1:50:300, get N-methyl-N-nitrosourea, fresh Mikania micrantha stem and mass Put the glucose solution with a fraction of 2% into a juice extractor to squeeze the juice, and collect the squeezed juice; after the above-mentioned straw-covered mixture is finished, remove the straw and allow natural ventilation for 19 days, and stir at 120r / min every 34 hours during the period of natural ventilation Remove the mixture after removing the straw for 15 minutes, and add juice equal to the biogas slurry in the mixture to the mixture after removing the straw every 6 days; after the above-mentioned natural...

example 3

[0024] In terms of parts by weight, take 55 parts of cow dung, 45 parts of peat, 15 parts of biogas slurry and 7 parts of discarded mushroom sticks, put them into the mixer, stir at 180r / min for 35min, and spread the stirred mixture on the cement In the pool, spread the thickness of 10cm, use straw to cover the mixture, the covering thickness is 3cm, and cover for 3 days; according to the mass ratio of 1:50:250, take N-methyl-N-nitrosourea, fresh Mikania micrantha stems and mass Put the glucose solution with a fraction of 2% into a juice extractor to squeeze the juice, and collect the squeezed juice; after the above-mentioned straw-covered mixture is finished, remove the straw, let it ventilate naturally for 15 days, and stir at 120r / min every 33 hours during the period of natural ventilation Remove the mixture after removing the straw for 14 minutes, and add juice equal to the biogas slurry in the mixture to the mixture after removing the straw every 5 days; after the above-me...

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Abstract

The invention discloses a preparation method of a microbial preparation for treating mikania micrantha kunth, and belongs to the technical field of comprehensive prevention and control of invasive alien harmful species. According to the present invention, cow dung, biogas slurry and waste mushroom sticks are used as a bacterial source, peat is used as a nutrient, flora is expanded by fermentation, N-methyl-N-nitrosourea is used as an inducer, the juice of fresh mikania micrantha kunth stem is used as a screening nutrient, target mixed bacteria are obtained, the strain using the fresh mikania micrantha kunth stem as the nutrient is obtained through enrichment culture, microorganisms are adsorbed by using calcined vermiculite soaked with glucose as a carrier, and coating with sodium alginate is performed, such that the microbial preparation for treating mikania micrantha kunth is prepared; and the microorganism in the prepared microbial preparation uses the mikania micrantha kunth stem as the nutrient, such that the asexual reproduction and the seed production of the mikania micrantha kunth are cut, the treatment effect on mikania micrantha kunth is good, and the problems of slow effect, high cost, low prevention and control efficiency and easy harm on other plants of the chemical agent are effectively solved.

Description

technical field [0001] The invention discloses a preparation method for treating mikania micrantha microbial preparations, and belongs to the technical field of comprehensive prevention and control of alien invasion pests. Background technique [0002] Mikania micrantha, also known as the small flower Eupatorium, is a plant of the genus Eupatorium in the Asteraceae family. It is a perennial herbaceous or slightly woody vine with trailing stems. It is native to Central and South America and is now widely distributed in Southeast Asia. It is a major threat to economic crops and forest vegetation. Noxious weeds. Mikania micrantha has strong vitality, fast growth, strong asexual reproduction ability, high seed yield and fast propagation speed, the number of seeds is large but small, and it can be spread over long distances with the help of wind, animals, insects and human activities. Stems have stronger light adaptation than leaves capacity and photosynthetic capacity, but the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P13/00
CPCA01N63/10Y02A90/40
Inventor 雷笑天宋家平李雪晴
Owner 雷笑天
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