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A kind of subculture method of tissue cultured seedlings of Ulmus chinensis

A technique for subculture and tissue culture of seedlings, which is applied in the fields of plant cultivation and biology, can solve the problems of less research on Ulmus chinensis and no reports on tissue culture research of Ulmus chinensis, and achieve reduced production costs, stable and stable multiplication coefficients, and solutions yellowing effect

Active Publication Date: 2019-01-18
上海菁艺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In summary, it can be seen that there are few studies on Ulmus chinensis, only a small amount of research on extracts, planting methods and grafting methods, and almost no reports on tissue culture of Ulmus chinensis

Method used

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  • A kind of subculture method of tissue cultured seedlings of Ulmus chinensis
  • A kind of subculture method of tissue cultured seedlings of Ulmus chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of elm subculture method, it carries out according to the following sequence of steps:

[0031] (1) Explant treatment: pretreat the collected explants, remove dead leaves and rotten leaves, cut off damaged and necrotic branches, soak them in 1‰ washing powder solution for 10 minutes, and use them under running water. Brush off the dust on the surface of the branches with a soft brush, put them in clean water for germination, and change the water once a day. After about 5 days, the branches will start to germinate and new branches will be pulled out.

[0032](2) Acquisition of sterile seedlings: when the length of the new branch is greater than 3cm, cut it off at a distance of 0.2cm from the base of the new branch, and cut off the petiole and leaves at a distance of 2-3mm from the base of the petiole, and soak in 1% washing powder 5min, rinse with running water for 30-60min, then disinfect with 75% alcohol for 20-30s on the ultra-clean workbench, rinse with steril...

Embodiment 2

[0035] Optimization of subculture basic medium. In order to solve the yellowing problem of tissue cultured seedlings in the process of subculture of Ulmus chinensis, the basic medium for subculture was improved and optimized. The specific components of the improved DKW mother solution are as follows:

[0036] CaCl 2 2H 2 O 134-164mg / L; MgSO 4 ·7H 2 O 666-814mg / L; KNO 3 1629-1991mg / L; (NH 4 ) 2 SO 4 1050-1284mg / L; KH 2 PO 4 239-292mg / L; Na 2 EDTA 40.9-49.9mg / L; FeSO 4 ·7H 2 O30.4-37.2mg / L; Ca(NO 3 ) 2 4H 2 O1771-2165mg / L; H 3 BO 3 4.3-5.3mg / L; MnSO 4 4H 2 O 30.2-36.9mg / L; Zn(NO 3 ) 2 ·6H 2 O 15.3-18.7mg / L; Na 2 Mo·4H 2 O 0.35-0.43mg / L; CuSO 4 ·5H 2 O0.23-0.28mg / L; inositol 90-110mg / L; niacin 0.9-1.1mg / L; thiamine hydrochloride 1.8-2.2mg / L; glycine 1.8-2.2mg / L.

[0037] Except that the subculture basic medium is replaced with improved DKW mother liquor, other steps and methods are the same as in Example 1, using improved DKW as the basic medium, the p...

Embodiment 3

[0039] Screening of subculture media. Using improved DKW as basic medium, the effects of different concentrations of 6-BA, KT and IBA on the subculture of Ulmus chinensis were screened. 14 bottles were inoculated for each treatment, and 10 plants were inoculated in each bottle, repeated 3 times. The test scheme is shown in Table 1, except that the addition of hormones is different from Example 1, and the rest of the method steps are the same as in Example 1.

[0040] Table 1 Effects of different concentrations of 6-BA, KT, and IBA on the subculture of Ulmus chinensis

[0041] Test No.

[0042] It can be seen from Table 1 that different concentrations of 6-BA, KT, and IBA have different effects on the subculture of Ulmus chinensis, and the effects are 6-BA>KT>IBA. The proliferation coefficient of Ulmus chinensis increases with the concentration of BA , but when the concentration is too high, the plants are prone to vitrification; different concentrations of KT have ...

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Abstract

The invention discloses a hemiptelea tissue culture seedling subculture method. The method includes the following sequential steps that 1, a hemiptelea explant is treated, wherein an explant branch is pruned, cleaned and put in clean water for germination accelerating; 2, an aseptic seedling is obtained, wherein axillary buds germinating on the explant branch are disinfected, an initial medium is inoculated with the axillary buds, and the aseptic seedling is obtained; 3, the aseptic seedling is subcultured, wherein the aseptic seedling is transferred into a subculture medium for subculture multiple times. The hemiptelea tissue culture rapid propagation method is provided for the first time, nutritional ingredients in the mediums are adjusted by adding coconut milk, and the yellowing phenomenon generated after multiple times of subculture of hemiptelea tissue culture seedlings is solved; the growth coefficient of subculture is remarkably increased; the subculture frequency of subculture is increased, and if the method that five-generation propagation and one-generation rooting are alternately carried out is adopted, hemiptelea subculture can be infinitely carried out theoretically. The production cost is reduced, and large-scale and industrialized production of hemiptelea is possible.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and relates to a method for plant tissue culture, in particular to a method for subculture of elm eucalyptus tissue culture seedlings, which belongs to the field of biotechnology. Background technique [0002] Ulmus is a small deciduous tree of the family Ulmaceae, with a height of 10 to 15 meters, or shrub-like; light-loving, cold-resistant, drought-resistant and barren, easy to grow in various soils, and can be used as a sand-fixing tree species. Strong adaptability, strong sprouting ability, slow growth rate. The wood is light brown, hard and delicate, and can be used to make agricultural tools and utensils; the bark fiber can be used as raw materials for artificial cotton, ropes, and sacks; the young leaves can be used as beverages; because the branches have thorns, they grow very fast, and often form shrubs. Therefore, it is also used as a tree species for hedges. The seeds can be extracte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张玉杰
Owner 上海菁艺生物科技有限公司