Bio-safety carbon quantum dot-loaded adriamycin complex as well as preparation method and application thereof

A biosafety, carbon quantum dot technology, applied in the field of medicine, can solve the problems of complex preparation process and potential safety hazards in the drug-loading system, and achieve the effects of simple synthesis process, low preparation cost and implementation conditions, and reduced toxic and side effects

Inactive Publication Date: 2017-09-01
汪涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a biosafety carbon quantum dot-loaded doxorubicin complex and its prepar

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  • Bio-safety carbon quantum dot-loaded adriamycin complex as well as preparation method and application thereof
  • Bio-safety carbon quantum dot-loaded adriamycin complex as well as preparation method and application thereof
  • Bio-safety carbon quantum dot-loaded adriamycin complex as well as preparation method and application thereof

Examples

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Embodiment 1

[0032] A biosafety carbon quantum dot-loaded doxorubicin complex, the preparation method of which comprises the following steps:

[0033] (1) Mix commercially available milk and deionized water at a volume ratio of 5:4, then heat at 180°C for 2 hours;

[0034] (2) Cool the solution obtained in step (1) to room temperature naturally, then centrifuge at 12000r / min for 30min, and take the supernatant;

[0035] (3) filtering the supernatant obtained in step (2) with a filter membrane of 0.22 μm to obtain a carbon quantum dot solution;

[0036] (4) vacuum lyophilize the carbon quantum dot solution obtained in step (3), then add deionized water to make a 12mg / mL aqueous solution;

[0037] (5) Dissolving doxorubicin hydrochloride powder in deionized water to make a 400 μg / mL aqueous solution;

[0038] (6) mixing the aqueous solution of carbon quantum dots obtained in step (4), the aqueous solution of doxorubicin obtained in step (5) and the PBS solution in a volume ratio of 1:1:2 t...

Embodiment 2

[0044] A biosafety carbon quantum dot-loaded doxorubicin complex, the preparation method of which comprises the following steps:

[0045] (1) Mix commercially available milk and deionized water at a volume ratio of 1:1, then heat at 180°C for 2 hours;

[0046] (2) Naturally cool the solution obtained in step (1) to room temperature, then centrifuge at 12000r / min for 45min, and take the supernatant;

[0047] (3) filtering the supernatant obtained in step (2) with a filter membrane of 0.22 μm to obtain a carbon quantum dot solution;

[0048] (4) vacuum lyophilize the carbon quantum dot solution obtained in step (3), then add deionized water to make a 12mg / mL aqueous solution;

[0049] (5) Dissolving doxorubicin hydrochloride powder in deionized water to make a 400 μg / mL aqueous solution;

[0050] (6) The carbon quantum dot aqueous solution obtained in step (4), the adriamycin aqueous solution obtained in step (5) and the PBS solution are mixed in a volume ratio of 1:2:5 to mak...

Embodiment 3

[0054] A biosafety carbon quantum dot-loaded doxorubicin complex, the preparation method of which comprises the following steps:

[0055] (1) Mix commercially available milk and deionized water at a volume ratio of 5:4, then heat at 180°C for 2 hours;

[0056] (2) Cool the solution obtained in step (1) to room temperature naturally, then centrifuge at 12000r / min for 30min, and take the supernatant;

[0057] (3) filtering the supernatant obtained in step (2) with a filter membrane of 0.22 μm to obtain a carbon quantum dot solution;

[0058] (4) vacuum lyophilize the carbon quantum dot solution obtained in step (3), then add deionized water to make a 12mg / mL aqueous solution;

[0059] (5) Dissolving doxorubicin hydrochloride powder in deionized water to make a 400 μg / mL aqueous solution;

[0060] (6) mixing the aqueous solution of carbon quantum dots obtained in step (4), the aqueous solution of doxorubicin obtained in step (5) and the PBS solution in a volume ratio of 3:2:3 t...

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Abstract

The invention discloses a bio-safety carbon quantum dot-loaded adriamycin complex. A preparation method of the bio-safety carbon quantum dot-loaded adriamycin complex comprises the following steps: (1) mixing milk and deionized water according to the volume ratio of (5-6):(4-5), then putting at a temperature of 160-200 DEG C and heating for 2-4 hours; (2) cooling to a room temperature, centrifuging for 30-45 minutes under 10000-12000 r/min and taking supernatant liquid; (3) filtering with a filter membrane to obtain a carbon quantum dot solution; (4) preparing a carbon quantum dot aqueous solution; (5) preparing an adriamycin aqueous solution; (6) mixing the carbon quantum dot aqueous solution, the adriamycin aqueous solution and a PBS solution and preparing a mixed solution with pH being equal to 7.4; (7) performing light-shading reaction and dialysis to prepare. The preparation method is simple, the adriamycin load rate is high, the prepared complex is high in bio-safety, the killing efficiency of tumor cells is significantly improved, the killing efficiency of normal cells is significantly reduced, and the bio-safety carbon quantum dot-loaded adriamycin complex can be used for tumor disease treatment.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a biosafety carbon quantum dot-loaded doxorubicin complex and its preparation method and application. Background technique [0002] Cancer is one of the important diseases that threaten human life and health. Millions of people die of cancer every year. The treatment of cancer is a topic that medical workers and researchers continue to explore. In addition to surgical treatment, chemotherapy is an important treatment method. There are many kinds of chemotherapeutic drugs for different types of cancer. Adriamycin is a kind of spectrum anti-cancer drug, which has a killing effect on a variety of tumor cells. It is widely used clinically in acute lymphoblastic leukemia, lymphoma, breast cancer, ovarian cancer, and lung cancer. , gastric cancer, liver cancer, head and neck squamous cell carcinoma, etc. However, due to the single way of uptake of doxorubicin by tumor ce...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/04A61K31/704A61K49/00A61P35/00
Inventor 汪涛袁一方顾斌郭斌
Owner 汪涛
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