Application of soybean transcription factor GmAREB3 in plant fat metabolism regulation

A plant and plant tissue technology, applied in the direction of angiosperms/flowering plants, applications, plant peptides, etc., can solve the problems of soybean GmAREB3 function unknown, no function description, small similarity, etc.

Inactive Publication Date: 2017-09-05
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Given that the soybean genome sequence has been completed, the GmAREB3 gene sequence is consistent with Glyma06g47220.2, and the promoter analysis shows that it is an ABA response element ...

Method used

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  • Application of soybean transcription factor GmAREB3 in plant fat metabolism regulation
  • Application of soybean transcription factor GmAREB3 in plant fat metabolism regulation
  • Application of soybean transcription factor GmAREB3 in plant fat metabolism regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the cDNA clone of soybean transcription factor GmAREB3 and the construction of plant expression vector

[0067] 1. Acquisition of transcription factor GmAREB3

[0068] High expression of GmAREB3 gene in a family of bZIP transcription factors was obtained in the analysis of fatty acid accumulation metabolism during soybean seed development. Therefore, the correlation between the gene and the regulation of lipid synthesis was studied.

[0069] Total RNA was extracted from soybean Heinong 44 seedlings, and the RNA was reverse-transcribed with reverse transcriptase to synthesize cDNA.

[0070] According to the information of GmAREB3 full-length cDNA sequence in the soybean genome sequence of PlantGDB, design primers, the primer sequences are as follows:

[0071] GmAREB3-up: 5'-ATGGGGTCTCAATGTGGTGGTG (SEQ ID NO: 3);

[0072] GmAREB3-dp: 5'-GAATGAAGCCGATGATGTTCGGC (SEQ ID NO: 4).

[0073] Using the cDNA of Heinong 44 as a template and using GmAREB3-up and Gm...

Embodiment 2

[0084] Example 2, the application of GmAREB3 in regulating the total oil or fatty acid content of plant tissues

[0085] 1. Obtaining recombinant Agrobacterium

[0086] The recombinant vector pGWB412-GmAREB3 obtained in 2 of Example 1 was introduced into Agrobacterium GV3101 by electric shock method to obtain recombinant bacteria.

[0087] The plasmid of the recombinant bacteria was extracted, and after sequencing, the plasmid was pGWB412-GmAREB3, and the recombinant bacteria containing the plasmid was named GV3101 / GmAREB3, namely the recombinant Agrobacterium.

[0088] 2. Acquisition and identification of transgenic GmAREB3 Arabidopsis

[0089] The recombinant Agrobacterium GV3101 / GmAREB3 was cultivated to the logarithmic phase, and then it was transformed into the flower of Arabidopsis thaliana (col-0) (seeds were purchased from Arabidopsis Biological Resource Center (ABRC)) by vacuum method. Harvest the seeds, sow the seeds on the MS screening medium containing kanamycin ...

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Abstract

The invention discloses application of a soybean transcription factor GmAREB3 in plant fat metabolism regulation. The invention provides application of optional one of 1) protein GmAREB3, 2) DNA molecules for encoding the protein GmAREB3 and 3) a recombinant vector, an expression kit, a transgenic cell line or a recombinant bacterium containing the DNA molecules for encoding the protein GmAREB3 in the regulation of plant tissue total fat content and/or the regulation of the content of fatty acid in plant tissue, and the amino acid sequence of the protein GmAREB3 is as shown in the sequence 2 in a sequence table. Experiments show that the transcription factor GmAREB3 and the coding gene thereof can regulate the fat content in plant seeds and can increase the fat content in the plant seeds after overexpression; the gene can increase and improve the fat components of crops especially oil plant seeds such as soybean seeds and has important theoretical and practical significance to the culture of high-fat varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of soybean transcription factor GmAREB3 in the regulation and control of vegetable oil metabolism. Background technique [0002] 71% of the fat in the human diet comes from plants. Among several major oil-producing crops in the world, the total oil production of soybean accounts for about 30%, ranking first in the world's vegetable oil production (Table 1). [0003] Table 1 shows the main oil-producing crops in the world [0004] [0005] The synthesis of fatty acids is one of the most important metabolic pathways in plants. It exists in any cell of plants and is necessary for growth and development. Blocking it leads to cell death, so a plant mutant that blocks fatty acid synthesis has not been found so far. [0006] Plants differ greatly from other eukaryotes in the enzymes involved in the fatty acid synthesis pathway. Synthesis of fatty acids with 16 or 18 c...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8243
Inventor 张劲松陈受宜牛素玲李擎天宋庆鑫张万科马彪林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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