Application of soybean transcription factor GmAREB3 in plant fat metabolism regulation

Abstract

The invention discloses application of a soybean transcription factor GmAREB3 in plant fat metabolism regulation. The invention provides application of optional one of 1) protein GmAREB3, 2) DNA molecules for encoding the protein GmAREB3 and 3) a recombinant vector, an expression kit, a transgenic cell line or a recombinant bacterium containing the DNA molecules for encoding the protein GmAREB3 in the regulation of plant tissue total fat content and / or the regulation of the content of fatty acid in plant tissue, and the amino acid sequence of the protein GmAREB3 is as shown in the sequence 2 in a sequence table. Experiments show that the transcription factor GmAREB3 and the coding gene thereof can regulate the fat content in plant seeds and can increase the fat content in the plant seeds after overexpression; the gene can increase and improve the fat components of crops especially oil plant seeds such as soybean seeds and has important theoretical and practical significance to the culture of high-fat varieties.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of soybean transcription factor GmAREB3 in the regulation and control of vegetable oil metabolism. Background technique [0002] 71% of the fat in the human diet comes from plants. Among several major oil-producing crops in the world, the total oil production of soybean accounts for about 30%, ranking first in the world's vegetable oil production (Table 1). [0003] Table 1 shows the main oil-producing crops in the world [0004] [0005] The synthesis of fatty acids is one of the most important metabolic pathways in plants. It exists in any cell of plants and is necessary for growth and development. Blocking it leads to cell death, so a plant mutant that blocks fatty acid synthesis has not been found so far. [0006] Plants differ greatly from other eukaryotes in the enzymes involved in the fatty acid synthesis pathway. Synthesis of fatty acids with 16 or 18 c...

AI Technical Summary

Problems solved by technology

Given that the soybean genome sequence has been completed, the GmAREB3 gene sequence is consistent with Glyma06g47220.2, and the promoter analysis shows that it is an ABA response element binding factor, but there is no functional description

The protein sequence similarity between soybean GmAREB3 and Arabidopsis AREB3 is less than 20%, so the function of soybean GmAREB3 is unknown

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