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Application of protein TaTAR2;1 in regulation of biomass and root development of plant

A plant root and protein technology, applied in the field of protein TaTAR2, can solve problems such as environmental pollution and resource waste, and achieve the effect of increasing plant biomass and promoting plant root development.

Active Publication Date: 2017-09-12
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, as much as 45–50% of nitrogen fertilizer is not absorbed by crops and lost into the environment, resulting in waste of resources and environmental pollution

Method used

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  • Application of protein TaTAR2;1 in regulation of biomass and root development of plant
  • Application of protein TaTAR2;1 in regulation of biomass and root development of plant
  • Application of protein TaTAR2;1 in regulation of biomass and root development of plant

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Experimental program
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Embodiment 1

[0075] Embodiment 1, transfer TaTAR2; 1 gene Arabidopsis acquisition and phenotypic identification

[0076] 1. Construction of recombinant vector and recombinant Agrobacterium

[0077] 1. Acquisition of TaTAR2; 1 gene

[0078] (1) The total RNA of the young leaves of Kenong 199 was extracted by the Trizol method, and the first-strand cDNA was reverse-transcribed from the total RNA by reverse transcriptase.

[0079] 2. Synthetic primer F1: 5'- TCTAGA ATGCCACTATGGCGAGTCTTC-3' (the underline is the recognition sequence of restriction endonuclease XbaⅠ) and R1: 5'- GTC GAC CCATTTGCCATTCGAAACTGCT-3' (the underline is the recognition sequence of restriction endonuclease SalI).

[0080] 3. Using the cDNA obtained in step 1 as a template, and using the F1 and R1 synthesized in step 2 as primers, carry out PCR amplification to obtain a double-stranded DNA molecule of about 1500 bp.

[0081] Reaction program: 98°C for 2min, (98°C for 30s, 58°C for 30s, 68°C for 45s) × 35 cycles, 7...

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Abstract

The invention an application of a protein TaTAR2;1 in the regulation of the biomass and the root development of a plant. The protein TaTAR2;1 is a protein a1 with the amino acid sequence represented by sequence 2 in a sequence table, or a fusion protein a2 obtained through connecting the N end or / and the C end of the protein represented by the sequence 2 in the sequence table with a marker, or a protein a3) obtained by substituting and / or deleting and / or adding one or more amino acid residues to the amino acid sequence represented by the sequence 2 in the sequence table and associated with the regulation of the biomass and / or the root development of the plant. Experiments prove that TaTAR2;1 transgenic Arabidopis thaliana has obviously higher visible lateral root quantity, total lateral root length, main root length, aboveground part biomass and root system biomass than wild Arabidopsis thaliana, so the protein TaTAR2;1 has important application values in the selection of new materials for regulating the biomass and / or the root development of the plant.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of protein TaTAR2; 1 in regulating plant biomass and root system development. Background technique [0002] Nitrogen is one of the most important macroelements necessary for plants. It is the main component of important compounds such as nucleic acid, protein, phospholipid, and chlorophyll in plants, and plays an important role in material metabolism and energy conversion. The lack of nitrogen in plants can lead to slow plant growth, reduced yield, reduced grain protein content of food crops, and reduced quality. The use of nitrogen fertilizers is an important reason for the substantial increase in crop yields in the past few decades. However, since the 1990s, while the rate of nitrogen fertilizer use in my country has continued to increase significantly, the growth of total grain production and per unit yield has slowed down or even stagnated. Compared with countrie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H5/06
CPCC07K14/415C12N15/8261
Inventor 童依平邵安马文英赵学强何雪
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI