CD44-shRNA/PEG-MZF-NPs/DDP nano-liposome and preparation method thereof

A PEG-MZF-NPS, nanoliposome technology, applied in the fields of medicine and genetic engineering, can solve the problem of allergic reaction of patients, achieve good dispersion, realize the integration of targeted diagnosis and treatment, and good effect of magnetocaloric effect

Inactive Publication Date: 2017-09-15
JIANGSU TAIZHOU PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, cisplatin currently used clinically is injected intravenously. After intravenous administration, due to the rapid increase in plasma DDP concentration and rapid diffusion to surrounding tissues, patients may experience loss of appetite, nausea, vomiting, diarrhea and other gastrointestinal symptoms and bone marrow symptoms. Inhibition, nephrotoxicity, neurotoxicity, allergic reactions and other toxic and side effects

Method used

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  • CD44-shRNA/PEG-MZF-NPs/DDP nano-liposome and preparation method thereof
  • CD44-shRNA/PEG-MZF-NPs/DDP nano-liposome and preparation method thereof
  • CD44-shRNA/PEG-MZF-NPs/DDP nano-liposome and preparation method thereof

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Embodiment

[0042] 1. Preparation and characterization of PEG-MZF-NPS / DDP complex

[0043] 1.1 Preparation of PEG-MZF-NPS / DDP complex

[0044] Take a certain amount of PEG-MZF-NPS magnetic fluid and mix it with an appropriate amount of DDP solution (the mass ratio of PEG-MZF-NPS to DDP is 40:1-80:1), add distilled water (the final concentration of PEG-MZF-NPS is 60μg / ml ), ultrasonically disperse for 15 min to make it fully react, filter, and the filtrate is the PEG-MZF-NPS / DDP complex, which is stored in a refrigerator at 4°C and protected from light for later use.

[0045] 1.2 Stability detection of PEG-MZF-NPS / DDP complex

[0046] The PEG-MZF-NPS / DDP complex was stored in a refrigerator at 4°C. After one week, a small amount of brown precipitate could be seen at the bottom of the tube, which could be resuspended after 1 min of ultrasonic dispersion, indicating that the complex had good suspension stability.

[0047] 1.3 TEM observation of PEG-MZF-NPS / DDP complex

[0048] Take a smal...

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Abstract

The invention belongs to the technical fields of medicines and genetic engineering and particularly relates to a CD44-shRNA / PEG-MZF-NPs / DDP nano-liposome as well as a preparation method and application thereof. According to the nano-liposome, a PEG-MZF-NPs nano-magnetic-particle is taken as a carrier and is loaded with DDP and a plasmid CD44-shRNA. The preparation method comprises the steps of firstly preparing a PEG-MZF-NPS / DDP compound, and mixing the PEG-MZF-NPS / DDP compound with the plasmid CD44-shRNA, so as to obtain the nano-liposome. The invention further discloses application of the nano-liposome in the preparation of drugs for treating ovarian cancer. The nano-liposome has relatively good proliferation inhibition and apoptosis induction effects to ovarian cancer cells under the action of an external alternating magnetic field, the effects are obviously superior to any single treatment, and the mechanism may be that the protein expression of CD44, Survivin, VEGF and Bcl-2 is reduced so as to inhibit cell proliferation, induce cell apoptosis, inhibit vascular proliferation or directly induce cell death.

Description

technical field [0001] The invention belongs to the technical fields of medicine and genetic engineering, and in particular relates to a CD44-shRNA / PEG-MZF-NPs / DDP nano liposome and a preparation method and application thereof. Background technique [0002] Ovarian cancer is one of the most common tumors in the female reproductive system, with a high degree of malignancy, and its mortality rate ranks first in gynecological tumors. Ovarian cancer often has no obvious symptoms in the early stage, and it is not easy to find early. More than 70% of the patients are already in the middle and late stages when they go to the doctor, and the 5-year survival rate is only 25%. At present, there is no specific and effective method or means for the early diagnosis of ovarian cancer. The treatment of ovarian cancer is currently mainly based on surgery followed by chemotherapy, but the side effects of conventional chemotherapy are relatively large, and the recurrence rate and metastasis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K33/24A61K41/00A61K9/127A61K47/60A61K47/69A61K9/00A61P35/00
CPCA61K48/0066A61K9/0009A61K9/127A61K33/24A61K41/0052A61K48/0008A61K2300/00
Inventor 林梅郭婷
Owner JIANGSU TAIZHOU PEOPLES HOSPITAL
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