Preparation method of high porosity nerve conduit material

A nerve conduit, high porosity technology, applied in medical science, prosthesis, etc., can solve the problems of slow degradation rate and poor nerve conduit porosity, etc., to improve enrichment and regeneration, shorten repair time, improve activity and recovery performance effect

Inactive Publication Date: 2017-09-15
常州市顺旭商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the problems of poor porosity and slow degradation rate of existing nerve guides, a preparation method of high-porosity nerve guide materials is provided

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Take Tremella and dry at 75°C for 2 hours to obtain dried Tremella, grind the dried Tremella through a 100-mesh sieve, and mix the dried Tremella with a chitinase solution with an enzyme activity of 25U / mL at a mass ratio of 1:50, After adjusting the pH to 5.0 with glacial acetic acid, heat in an oil bath at 40°C for 1 hour, then raise the temperature to 95°C, and inactivate the enzyme by heat preservation for 45 minutes. Centrifuge for 10 minutes, collect the supernatant, mix the supernatant with n-butanol at a volume ratio of 1:8, treat it in an ice-water bath at 0°C for 2 hours, filter to obtain a filter cake, vacuum freeze-dry and grind through 100 mesh Sieve to get white fungus extract powder; by weight, weigh 5 parts of white fungus extract powder, 45 parts of mass fraction 10% acetic acid solution, 5 parts of chitosan and 45 parts of deionized water and place in a beaker, stir and mix to obtain Mixed solution, filter the mixed solution through a microporous membr...

example 2

[0022] Take the white fungus and dry it at 77°C for 3 hours to get the dried white fungus, grind the dried white fungus through a 100-mesh sieve, mix the dried white fungus with a chitinase solution with an enzyme activity of 25 U / mL at a mass ratio of 1:50, After adjusting the pH to 5.0 with glacial acetic acid, heat in an oil bath at 42°C for 1 hour, then raise the temperature to 97°C, and inactivate the enzyme by heat preservation for 52 minutes. Centrifuge for 12 minutes, collect the supernatant, mix the supernatant with n-butanol at a volume ratio of 1:8, treat in an ice-water bath at 4°C for 3 hours, filter to obtain a filter cake, vacuum freeze-dry and grind through 100 mesh Sieve to get Tremella extract powder; by weight parts, weigh 6 parts of Tremella extract powder, 47 parts of mass fraction 10% acetic acid solution, 8 parts of chitosan and 47 parts of deionized water in a beaker, stir and mix to obtain Mixed solution, filter the mixed solution through a microporous...

example 3

[0024]Take the white fungus and dry it at 80°C for 3 hours to get the dried white fungus, grind the dried white fungus through a 100-mesh sieve, mix the dried white fungus with a chitinase solution with an enzyme activity of 25 U / mL at a mass ratio of 1:50, After adjusting the pH to 5.0 with glacial acetic acid, heat in an oil bath at 45°C for 2 hours, then raise the temperature to 105°C, and inactivate the enzyme by heat preservation for 60 minutes. Centrifuge for 15 minutes, collect the supernatant, mix the supernatant with n-butanol at a volume ratio of 1:8, treat in an ice-water bath at 5°C for 3 hours, filter to obtain a filter cake, vacuum freeze-dry and grind through 100 mesh Sieve to get white fungus extract powder; by weight, weigh 8 parts of white fungus extract powder, 50 parts of mass fraction 10% acetic acid solution, 10 parts of chitosan and 50 parts of deionized water in a beaker, stir and mix to obtain Mixed solution, filter the mixed solution through a micropo...

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Abstract

Belonging to the field of medical material preparation, the invention relates to a preparation method of a high porosity nerve conduit material. The method adopts tremella as the raw material, tremella polysaccharide is extracted and loaded to the inside of chitosan to form microspheres, then coating modification and dispersion are carried out to prepare a nerve conduit, when the coating shell chitosan material is degraded by itself and absorbed by the human body, the internal tremella polysaccharide is released to eliminate free radicals in the nerve conduit so as to improve the anti-lipid peroxidation effect, also the load is fixed to the material surface to repair injured nerve factors, promote nerve cell regeneration activity and guide nerve regeneration, and shorten the nerve cell repair time. At the same time, according to the tremella modified microspheres prepared by the invention, the chitosan coated on the surfaces releases internal materials after decomposition, so that a uniform connected pore structure can be formed in the nerve conduit, and a structure similar to the nervous tissue internal environment can be constructed, thus effectively improving the nerve cell enrichment and regeneration, and improving the material activity and restorability after transplantation.

Description

technical field [0001] The invention relates to a preparation method of a high-porosity nerve guide material, which belongs to the field of preparation of medical materials. Background technique [0002] Peripheral nerve injury is a common disabling disease in clinical practice. Compared with the central nervous system, the peripheral nerve structure is simpler and easier to regenerate. With the development of microsurgical equipment and technology, the clinical treatment effect of peripheral nerve injury is continuously improving. . For short defect gaps, it can be directly repaired with fine suture or small gap sleeve; for long defect distances, nerve tissue transplantation or nerve catheter bridging should be used. Nerves with different diameters; ②The length can be adjusted, and the anastomosis can be sutured without tension; ③Prevent the invasion of surrounding fibrous scar tissue and guide the directional growth of axons; ④It is conducive to the accumulation of endoge...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/22A61L27/36A61L27/54A61L27/56A61L27/58
Inventor 江建荣季美
Owner 常州市顺旭商贸有限公司
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