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Yeast dd12-7 strain and its application

A technology of yeast and yeast, which is applied in the field of yeast DD12-7 strain, can solve the problem of no killing, etc., and achieve the effect of short fermentation cycle, simple nutritional requirements and high cost advantages

Inactive Publication Date: 2020-11-10
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The killing toxin secreted by killing yeast is called killing factor, which can kill sensitive yeast strains, but has no killing effect on killing yeast itself, that is to say, it has immunity

Method used

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  • Yeast dd12-7 strain and its application
  • Yeast dd12-7 strain and its application
  • Yeast dd12-7 strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] YPD medium: 1L medium contains: glucose 2%, peptone 2%, yeast powder 1%, NaCl 2% and glycerin 15%, the medium is prepared with citric acid-disodium hydrogen phosphate buffer solution, and the pH value is adjusted to 4.5 .

[0017] Assay medium: 1.0% (w / v) yeast powder, 2.0% (w / v) glucose, 2.0% (w / v) peptone, 2.5%-3.5% (w / v) agar, 1.5 mg / 100mL methylene blue , using 0.05 M citric acid-disodium hydrogen phosphate buffer to control pH 4.5, and the buffer was prepared with distilled water.

[0018] 1. Collect samples: collect the surface seawater at the Yalu River estuary in Dandong, Liaoning, and add it to liquid YPD medium for shaking culture for 3 days. Spread 0.1ml of the liquid culture medium on a solid YPD plate, and pick a single colony after 3 days of cultivation for preliminary screening.

[0019] 2. Primary screening: Candida albicans ( Candida albicans ) as a sensitive bacteria, the cell content was 10 7 ~10 8 Cells / mL sensitive bacteria are dipped and spre...

Embodiment 2

[0028] The production of embodiment 2 killing factor

[0029] cultured in medium Williopsis sp.DD12-7 causes the bacteria to secrete the anti-Candida albicans killing factor, so that after the anti-Candida albicans killing factor has accumulated in the bacteria and the medium, the culture medium is centrifuged to collect the supernatant, which is the killing factor crude extract.

[0030] The medium is a culture medium for killing factor, which contains 2% glucose, 2% peptone, 1% yeast powder, 2% NaCl and 15% glycerin. The medium is prepared with citric acid-disodium hydrogen phosphate buffer solution, and the pH is adjusted to 4.5. The culture is shaking culture, carried out at 28°C and pH 4.5, with a rotation speed of 140 rpm, and a fermentation time of 72 hours.

[0031] Purification process: the fermented liquid is concentrated through a 10 kDa ultrafiltration membrane, then passed through a DEAE Sepharose Fast Flow ion exchange column for anion exchange, and the colle...

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Abstract

The invention discloses a kind of saccharomyces, which is Saccharomyces spp. Williopsis sp. ) DD12‑7 strain, whose preservation number is CGMCC No.9833, has the ability to secrete anti-candida albicans killing factor. The invention also discloses the application of the yeast. The yeast strain DD12-7 of the present invention has the characteristics of simple nutritional requirements and short fermentation period, and the use of the strain to produce anti-candida albicans killing factor has high cost advantages and good application prospects.

Description

technical field [0001] The invention relates to yeast DD12-7 strain and application thereof. Background technique [0002] In 1963, Bevan and Makower for the first time in the preserved Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) found that there is a phenomenon of mutual killing among yeast strains, and then found a yeast with killing activity, and the exotoxin secreted by it can inhibit or kill wild yeast. The killing toxin secreted by killing yeast is called killing factor, which can kill sensitive yeast strains, but it has no killing effect on killing yeast itself, that is to say, it has immunity. Judging from the purified and characterized virophilic toxins, the virophilic toxins produced by yeast killing yeast are mostly proteins or glycoproteins, which are heat-labile and sensitive to proteases in terms of biological characteristics. The killing factor secreted by killing yeast can be used as an antifungal agent to inhibit some pathogenic yeast and yeast-l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C07K14/39C12R1/645
CPCC07K14/39C12N1/145C12R2001/645
Inventor 于刚王麟谭春明杨少玲杨贤庆
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI