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A kind of staining agent for rapid color development after cell staining in urine and its application method

A dye and cell technology, applied in the field of in vitro diagnosis, can solve the problems of complex preparation process, long dyeing time, easy aggregation, etc., and achieve the effects of simple operation, high detection efficiency, and short color development time.

Active Publication Date: 2020-02-04
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a staining agent for rapid color development after staining cells in urine and its use method, aiming to solve the problem of complex sample preparation process and difficult staining of existing urine cell staining methods. It takes a long time, the shape of red blood cells is affected by the staining solution and is prone to aggregation, and it is not conducive to automatic detection

Method used

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  • A kind of staining agent for rapid color development after cell staining in urine and its application method
  • A kind of staining agent for rapid color development after cell staining in urine and its application method
  • A kind of staining agent for rapid color development after cell staining in urine and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1. Preparation of staining agent Reagent A:

[0065] Add 5.00g of methanol, 95.00g of ethylene glycol and 1.20g of neutral red to the brown reagent bottle in order to obtain the first mixed solution A1; after that, place the brown reagent bottle containing the above-mentioned first mixed solution A1 on the shaker , Dissolved at a medium speed for 3 hours, so that the drug was completely dissolved, and the second mixed solution A2 was obtained; then, the above-mentioned second mixed solution A2 was filtered with a 0.22um filter membrane, and the filtered solution was marked as reagent A, and placed in a brown bottle save in .

[0066] Reagent B:

[0067] Add 100.00g of purified water, 4.20g (200mM) of 1,3-bis[(tris(hydroxymethyl)methylamino]propane (Bis-tris), and 8.00g of sodium propionate into the reagent bottle, and fully dissolve to obtain the first A mixed solution B1; add propionic acid to the first mixed solution B1 to make the pH of the solution B1 within the r...

Embodiment 2

[0071] 1. Preparation of staining agent Reagent A:

[0072] Add 100.00g of ethylene glycol and 1.20g of neutral red to the brown reagent bottle in sequence to obtain the first mixed solution A1; after that, place the brown reagent bottle containing the above first mixed solution A1 on a shaker and dissolve at a medium speed After 3 hours, the drug was completely dissolved to obtain the second mixed solution A2; then, the above-mentioned second mixed solution A2 was filtered with a 0.22um filter membrane, and the filtered solution was marked as reagent A, and stored in a brown bottle.

[0073] Reagent B:

[0074] Add 100.00g of purified water, 2.10g (50mM) of 2-(N-morpholine)ethanesulfonic acid monohydrate (MES), 4.00g of sodium propionate, 4.00g of EDTA-K into the reagent bottle 2 After fully dissolving, the first mixed solution B1 is obtained; the pH value of the first mixed solution B1 is adjusted to within the range of 6.00±0.05 (25±1°C) with propionic acid; Filter, label...

Embodiment 3

[0078] 1. Preparation of staining agent Reagent A:

[0079] Add 100g of glycerol and 0.6g of neutral red to the brown reagent bottle in sequence to obtain the first mixed solution A1; after that, place the brown reagent bottle containing the above-mentioned first mixed solution A1 on a shaker and dissolve it at a medium speed for 3.5 hours, the medicine was completely dissolved to obtain the second mixed solution A2; then the above-mentioned second mixed solution A2 was filtered with a 0.22um filter membrane, and the filtered solution was marked as reagent A, and placed in a brown bottle for preservation.

[0080] Reagent B:

[0081] Add 100g of purified water, 0.61g (50mM) of Tris (Tris), 8g of sodium propionate and 1.9g of citric acid into the reagent bottle, and fully dissolve to obtain the first mixed solution B1; Adjust the pH value of a mixed solution B1 to within the range of 6.70±0.05 (25±1°C); then use a 0.22 μm filter membrane to filter the above-mentioned second mixe...

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Abstract

The invention provides a staining agent capable of rapidly developing after staining of cells in urine and a use method of the staining agent. The staining agent comprises a reagent A and a reagent B, the weight ratio of the reagent A to the reagent B is 0.25-4, the reagent A in the staining agent comprises 0.6-1.2g of neutral red dyes and 100g of dye solvents, the reagent B in the staining agent comprises, by weight, 10mM-600mM buffering agents, 0.1-8g of cell membrane damage agents, 0-4g of complex agents, 0.01-10g of pH (potential of hydrogen) conditioning agents and 100g of solvents. According to the staining agent, the cells in the urine can be directly stained without being pretreated, the staining agent is short in developing time, simple to operate and high in detection efficiency and can not affect forms of red blood cells with pathological significance, samples of the red blood cells of high concentration cannot be gathered, the accuracy of detection results is improved, and automatic detection can be achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a staining agent for rapid color development after staining of cells in urine and a use method thereof. Background technique [0002] The formed components in urine include red blood cells, white blood cells, casts, epithelial cells and other components. Formed component analysis is of great significance to the detection of diseases in the urinary system, adjacent reproductive system and other systems. A large number of studies have shown that crystals and yeast in urine can interfere with the analysis of urine red blood cells, a large number of mucus filaments and hyphae can cause false positives in cast counts, white blood cells and small phagocytes, and white blood cells and large red blood cells with inconspicuous nuclei, White blood cells are similar in shape to trichomonas, spores, and red blood cells, while hyaline casts and sperm are colorless, and the probability of fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 孟令敏赵亚荣何浩会
Owner DIRUI MEDICAL TECH CO LTD
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