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Preparation method of plasma-induced rhamnolipid emulsified asphalt

A rhamnolipid and plasma technology, applied in biochemical equipment and methods, mutant preparation, microbial-based methods, etc., to achieve good emulsification effect, strong surface activity, and high yield

Inactive Publication Date: 2017-09-22
安徽省中日农业环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the application of rhamnolipids as emulsifiers in emulsified asphalt

Method used

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  • Preparation method of plasma-induced rhamnolipid emulsified asphalt
  • Preparation method of plasma-induced rhamnolipid emulsified asphalt

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Embodiment 1

[0024] A preparation method for plasma mutagenesis of rhamnolipid emulsified asphalt, including the following steps:

[0025] Step 1: Pseudomonas aeruginosa is inoculated on the slant medium, placed in the incubator, the culture temperature is 37℃, and the culture is 24h;

[0026] Step 2: Connect the colonies on the slant medium to the seed medium, 37°C, and shaker rotation speed 200r·min -1 , Cultivate for 24h;

[0027] Step 3: Use an inoculation needle to gently scrape the colonies on the seed culture medium into a container containing 50L of normal saline, place it in a shaker and shake for 30 minutes, filter with a funnel stuffed with sterile absorbent cotton, and absorb 10mL of the filtrate into a sterile In the petri dish, use normal pressure and room temperature plasma mutagenesis treatment for 2 minutes, and then dilute to 8% (v / v) with physiological saline. Spread the diluted solution evenly on the plate medium, and incubate the plate for 4 days at 37°C;

[0028] Step 4: Pick...

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Abstract

The invention relates to a preparation method of plasma-induced rhamnolipid emulsified asphalt, which belongs to the technical field of the preparation of asphalt. The preparation method comprises the following steps: inoculating aerugo pseudomonas at an inclined culture medium, inoculating at a seed culture medium, inducing by utilizing plasma, inoculating at a plate culture medium, inoculating at a fermentation culture medium, extracting by utilizing acetic acid and preparing the emulsified asphalt. The rhamnolipid has higher surface activity and emulsifying capacity, can be completely degraded by organisms and is environment-friendly. The plasma-induced rhamnolipid emulsified asphalt prepared by adopting the preparation method of the invention is good in emulsification effect, good in stability and good in filtration reducing property and rejection capability. The plasma is adopted as a mutagenic agent, and induces the aerugo pseudomonas, and the yield of the rhamnolipid is higher.

Description

Technical field [0001] The invention belongs to the technical field of asphalt preparation, and specifically relates to a preparation method of plasma mutagenized rhamnolipid emulsified asphalt. Background technique [0002] Emulsified asphalt is a uniform and stable emulsion formed by heating the viscous asphalt to a fluid state, and then forming microdroplets dispersed in the water with emulsifier and stabilizer under the action of mechanical force. Emulsified asphalt is a thermodynamically unstable system. Its stability is caused by various effects produced by external emulsifiers, emulsion stabilizers, etc. The current emulsified asphalt has the problem of insufficient stability. [0003] Rhamnolipid is a kind of glycolipid biosurfactant with strong surface activity and emulsifying ability. It can be completely degraded by organisms and is environmentally friendly. It is used in petroleum exploration, bioremediation, agricultural production, food and medicine, etc. Potential ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L95/00C08K5/1545C12N15/01C12N13/00C12P19/44C12R1/385
CPCC08K5/1545C08L2201/06C12N13/00C12N15/01C12P19/44C08L95/00
Inventor 章云张贝尼
Owner 安徽省中日农业环保科技有限公司