Intestinal membrane protein polypeptide extracting process

Abstract

An intestinal membrane protein polypeptide extraction process belongs to the technical field of biopharmaceuticals and feed production. After the small intestinal mucosal fluid obtained by scraping is hydrolyzed with salt, the filtrate and filter residue are obtained by centrifugal filtration; the filtrate is adsorbed, eluted and precipitated to obtain heparin sodium; the filter residue is subjected to enzymatic hydrolysis with protease. , and obtain intestinal membrane protein by drying. The present invention effectively separates the salt-degraded heparin solution from the intestinal membrane protein, and because the heparin water is clarified after separation, the resin adsorption is more convenient, and the yield of heparin is improved; secondly, manpower is saved, and it can meet the needs of large-scale production. After the invention adopts the centrifugal filtration technology instead, the solid content of the intestinal membrane protein can reach about 35%, which can greatly increase the economic benefit of production, and is also conducive to improving the environment.

Description

technical field [0001] The invention belongs to the technical field of biological pharmacy and feed production. Background technique [0002] my country is the most important natural casing production and export country in the world, and more than 60% of the casings in the world need to be processed and exported in my country. The small intestinal mucosa produced in the production of casings can be processed to obtain crude heparin and intestinal membrane protein feed. The current production process of heparin sodium is as follows: [0003] 1. Acceptance of pig small intestine (to ensure the quality of raw materials of pig small intestine, mainly freshness). [0004] 2. Mucosa extraction (small intestinal mucosa obtained by scraping). [0005] 3. Enzymatic hydrolysis: add salt, 3.5% to 4% of the mucous membrane, adjust the pH to 8.5 to 9, then raise the temperature to 55 to 58°C, and perform salt hydrolysis for 1 hour; then add 2 to 3 proteases per small intestine gram...

AI Technical Summary

Problems solved by technology

[0012] The above process is currently adopted by most manufacturers. The biggest disadvantage is that the by-product intestinal membrane protein is all turned into waste water for disposal, which not only wastes valuable intestinal membrane protein resources, but also pollutes the environment.