Wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof

A technology of molecular markers and ear germination, which is applied in the field of molecular biology and crop breeding, can solve the problems affecting the storage of wheat grains, the deterioration of the quality of wheat processed food, and the reduction of grain production.

Inactive Publication Date: 2017-09-29
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The ear germination of wheat grains before harvest will not only reduce the grain yield of a large area, but also lead to a rapid increase in the activity of enzymes related to hydrolysis in wheat grains, reducing the content of stored su

Method used

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  • Wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof
  • Wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof
  • Wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1 Mapping of Candidate SNP Sites and Acquisition of Molecular Markers for Anti-earing and Germination of Chinese Landraces of Wheat

[0075] 1. Collect local varieties of wheat from China's top ten wheat regions, which were planted in Ya'an Farm of Sichuan Agricultural University in 2012, Wenjiang Experimental Base of Sichuan Agricultural University and Chongzhou Modern Industrial Base in 2013, and Wenjiang Experimental Base of Sichuan Agricultural University in 2014 The base and planted in Chongzhou Modern Industrial Base of Sichuan Agricultural University in 2015.

[0076] 2. Identification of panicle germination

[0077] Harvest wheat seeds at the wax maturity stage, hang them in a cool and ventilated place to dry naturally for 7 days, and then perform manual threshing. Spread filter paper wetted with 3-5ml distilled water in a covered plastic Petri dish for germination test. The test design is repeated three times, with 50 seeds in each repetition. Pick ou...

Embodiment 2

[0099] Example 2 Application of molecular markers of the present invention in selection of candidate sites for ear germination resistance

[0100] 1. A total of 138 main varieties from various wheat regions in China were collected and planted in the Chongzhou Modern Agricultural Base of Sichuan Agricultural University from 2015 to 2016. The seeds were harvested at the wax ripening stage for identification of germination rate.

[0101] 2. Carry out KASP marker detection on the main varieties harvested. The specific method is: extract the total DNA of the leaf genome at the three-leaf stage; use the genomic DNA of 138 main varieties and 16 known genotype local varieties (controls) as substrates, PCR amplification was carried out with the developed KASP marker primers respectively, and the primers were:

[0102] AX-111578083:

[0103] Forward primer AL1: 5'-3'GAAGGTGACCAAGTTCATGCTGATCGGTAGCTTCCCCCa (SEQ ID NO:4)

[0104] Forward primer AL2: 5'-3'GAAGGTCGGAGTCAACGGATTGATCGGTAGCT...

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Abstract

The invention discloses wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof in the field of wheat molecular breeding. Three KASP markers (AX-111578083, AX-111624595 and AX-110772653) in the third homologous group of wheat, developed on the basis of an SNP marker and a flanking sequence of the SNP marker, are capable of accurately typing preharvest sprouting resistance candidate loci and predicting the preharvest sprouting resistance of the wheat, thereby more conveniently identifying and screening preharvest sprouting resistance materials under laboratory conditions. By using a molecular marker-assisted selective breeding technique, the influences of environmental factors and personal errors on phenotype identification can be effectively avoided, and the molecular marker-assisted selective breeding technique can be combined with phenotype identification for use. According to the three KASP markers, strongly selected loci can be provided, the accuracy of wheat preharvest sprouting resistance breeding material selection is improved, and the target of wheat preharvest sprouting resistance molecular marker-assisted selective breeding is achieved.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and crop breeding, in particular to KASP molecular markers related to wheat anti-ear germination traits and applications thereof. Background technique [0002] Wheat is the third largest food crop in the world, second only to rice and corn, and is widely grown worldwide. Pre-harvest Sprouting (PHS) refers to a phenomenon in which wheat germinates on ears before harvest when it encounters continuous rain before harvest. Ear germination is a worldwide disaster. According to reports, major wheat-growing countries in the world such as Canada, Japan, Australia, and China have serious ear germination hazards. There are 10 major wheat producing areas in China, mainly in the central and southeastern regions of China, such as the middle and lower reaches of the Yangtze River, and the southwestern wheat region. For example, ear germination often occurs in the northern winter wheat region. [0...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王际睿周勇唐豪
Owner SICHUAN AGRI UNIV
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