Wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof

Abstract

The invention discloses wheat preharvest sprouting resistance character-related KASP molecular markers and application thereof in the field of wheat molecular breeding. Three KASP markers (AX-111578083, AX-111624595 and AX-110772653) in the third homologous group of wheat, developed on the basis of an SNP marker and a flanking sequence of the SNP marker, are capable of accurately typing preharvest sprouting resistance candidate loci and predicting the preharvest sprouting resistance of the wheat, thereby more conveniently identifying and screening preharvest sprouting resistance materials under laboratory conditions. By using a molecular marker-assisted selective breeding technique, the influences of environmental factors and personal errors on phenotype identification can be effectively avoided, and the molecular marker-assisted selective breeding technique can be combined with phenotype identification for use. According to the three KASP markers, strongly selected loci can be provided, the accuracy of wheat preharvest sprouting resistance breeding material selection is improved, and the target of wheat preharvest sprouting resistance molecular marker-assisted selective breeding is achieved.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and crop breeding, in particular to KASP molecular markers related to wheat anti-ear germination traits and applications thereof. Background technique [0002] Wheat is the third largest food crop in the world, second only to rice and corn, and is widely grown worldwide. Pre-harvest Sprouting (PHS) refers to a phenomenon in which wheat germinates on ears before harvest when it encounters continuous rain before harvest. Ear germination is a worldwide disaster. According to reports, major wheat-growing countries in the world such as Canada, Japan, Australia, and China have serious ear germination hazards. There are 10 major wheat producing areas in China, mainly in the central and southeastern regions of China, such as the middle and lower reaches of the Yangtze River, and the southwestern wheat region. For example, ear germination often occurs in the northern winter wheat region. [0...

AI Technical Summary

Problems solved by technology

[0003] The ear germination of wheat grains before harvest will not only reduce the grain yield of a large area, but also lead to a rapid increase in the activity of enzymes related to hydrolysis in wheat grains, reducing the content of stored substances in the grains, the testing weight, and flour yield. Decrease in wheat flour rate and falling number (Falling number), resulting in deterioration of the quality of various wheat processed foods, and will affect the storage of wheat grain

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