A method for analyzing the radial distribution of amino acids in mulberry silk fibroin
A silk fibroin, radial distribution technology, applied in the field of silk, can solve the problems of protein mishydrolysis/enzymolysis, inaccurate analysis results, easy damage to amino acids, etc., to achieve mild working conditions, improve degumming efficiency, and reduce damage.
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Embodiment 1
[0033] A method for analyzing radial distribution of amino acids in mulberry silk fibroin protein, comprising the following steps:
[0034] 1) Take mulberry silk as a sample, wash it with deionized water, remove surface pollutants, and dry it.
[0035] 2) The dried sample was mixed with 0.5wt% Na at a bath ratio of 1:100 2 HPO 4 and 1wt%C 17 h 35 Boil in the mixed solution of COONa 30min, carry out degumming treatment, totally degumming twice;
[0036] 3) After degumming, the sample is rinsed with deionized water, and placed in an oven at 60° C. to obtain dried silk fibroin fibers.
[0037] 4) Take silk fibroin fibers with similar diameters and divide them into five groups A, B, C, D, and E with equal mass, wherein group A is used as a control group without any treatment.
[0038] 5) Put groups B, C, D, and E into the pre-prepared 1.5g / L trypsin solution at a bath ratio of 1:40, control the reaction temperature at 48°C, the pH of the solution at 8, and the reaction time f...
Embodiment 2
[0046] A method for analyzing radial distribution of amino acids in mulberry silk fibroin protein, comprising the following steps:
[0047] 1) Take mulberry silk as a sample, wash it with deionized water, remove surface pollutants, and dry it.
[0048] 2) The dried sample was mixed with 0.4wt% Na at a bath ratio of 1:95 2 HPO 4 and 0.8 wt% C 17 h 35 Boil in the mixed solution of COONa 35min, carry out degumming treatment, totally degumming twice;
[0049] 3) After degumming, the sample is rinsed with deionized water and put into an oven at 55° C. to obtain dried silk fibroin fibers.
[0050] 4) Take silk fibroin fibers with similar diameters and divide them into five groups A, B, C, D, and E with equal mass, wherein group A is used as a control group without any treatment.
[0051] 5) Put groups B, C, D, and E into the pre-prepared 1.5g / L trypsin solution at a bath ratio of 1:40, control the reaction temperature at 45°C, the pH of the solution at 7, and the reaction time ...
Embodiment 3
[0059] A method for analyzing radial distribution of amino acids in mulberry silk fibroin protein, comprising the following steps:
[0060] 1) Take mulberry silk as a sample, wash it with deionized water, remove surface pollutants, and dry it.
[0061] 2) The dried sample was mixed with 0.6wt% Na at a bath ratio of 1:105 2 HPO 4 and 1.2 wt% C 17 h 35 Boil in the mixed solution of COONa 25min, carry out degumming treatment, totally degumming twice;
[0062] 3) After degumming, the sample is rinsed with deionized water and placed in an oven at 65° C. to obtain dried silk fibroin fibers.
[0063] 4) Take silk fibroin fibers with similar diameters and divide them into five groups A, B, C, D, and E with equal mass, wherein group A is used as a control group without any treatment.
[0064] 5) Put groups B, C, D, and E into the pre-prepared 1.5g / L trypsin solution at a bath ratio of 1:40, control the reaction temperature at 50°C, the pH of the solution at 9, and the reaction tim...
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