Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of drug carrier with double response of magnetic-ph value

A dual-response, drug-based technology, applied in the direction of non-active ingredient medical preparations, active ingredient-containing medical preparations, pharmaceutical formulations, etc., can solve the problems of limited clinical application and low bioavailability of hydrophobic drugs, and achieve cost The effect of low cost and simple preparation method

Active Publication Date: 2020-03-17
JINAN UNIVERSITY
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The low bioavailability of hydrophobic drugs limits its clinical application to a certain extent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of drug carrier with double response of magnetic-ph value
  • A kind of preparation method of drug carrier with double response of magnetic-ph value
  • A kind of preparation method of drug carrier with double response of magnetic-ph value

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation of Fe by Chemical Co-precipitation 3 o 4 Nanoparticles. Will Fe 2+ and Fe 3+ Mix according to the ratio of 1:2, add excess ammonia water, stir at a certain temperature (about 50°C) for 30 minutes, separate and precipitate with a magnet, wash with 0.5% ammonia water, and precipitate, add appropriate amount of deionized water and 0.5g polyethylene glycol, Ultrasonic mixing, heating and aging at 80°C for 30 min (600 r / min), repeated washing with deionized water, to obtain black ferrofluid 3 o 4 The nanoparticles were put into hydrochloric acid solution (0.1M), dispersed by ultrasonic, and washed three times with deionized water. Preparation of SiMNP composite nanoparticles. Take 0.01g clean Fe 3 o 4 Nanoparticles, put into 100ml ethanol solution (the volume ratio of ethanol to deionized water is 4:1), stir mechanically, add 1ml ammonia water (25%) dropwise, then add 0.1ml tetraethyl orthosilicate dropwise, stir at room temperature for 12 h ; Apply a ma...

Embodiment 2

[0051] Preparation of CDMNP magnetic composite nanomaterials. Dissolve 2.5g of β-CD in 50ml of dimethylformamide, add 0.5g of sodium hydride, stir at room temperature for 15 min, filter to obtain a white transparent solution, add 4ml of KH-560, and stir at 90°C for 5h under nitrogen protection. Cool to room temperature to obtain modified β-CD; add 1g SiMNP, add dropwise 1.5ml ammonia water (25%), stir at room temperature for 12h, enrich and suck out with magnet, rinse with dimethylformamide, deionized water and ethanol three times in sequence, Vacuum-dried at 50°C for 12 h to obtain brown-black CDMNP magnetic composite nanomaterials.

Embodiment 3

[0053] Preparation of CDMNP-PEG-CD vectors. Take an appropriate amount of CDMNP magnetic composite nanoparticles, place them in 20% polyethylene glycol solution, and stir mechanically at room temperature for 24 hours; the product is enriched and sucked out, washed with water, and dried in vacuum to obtain CDMNP-PEG composite materials. Take 0.05g of CDMNP-PEG composite material, place in 0.5g of β-CD saturated solution of dimethylformamide, ultrasonic at room temperature for 10 min, and stand overnight at 4°C; Wash each once, centrifuge for 10 min (3200 r / min), and collect the precipitate by magnetic adsorption; this process is repeated 3 times to remove residual β-CD and CDMNP-PEG composites. The precipitate was vacuum-dried and stored for future use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of synthesis of biological materials, and in particular discloses a preparation method of a drug carrier having magnetism-pH value double-response. The preparation method comprises the following steps: S1, mixing Fe3O4 nanoparticles with ethanol and water, adding tetraethyl orthosilicate and implementing a reaction so as to obtain Fe3O4 @ SiO2 composite nanoparticles; S2, modifying [beta]-CD with the addition of a silane coupling agent, so that modified [beta]-CD is obtained; S3 , promoting a reaction between the composite nanoparticles in the step S1 and the modified [beta]-CD in the step S2, so that a magnetic composite nano-material is obtained; S4, adding the magnetic composite nano-material in the step S3 to an ethylene glycol solution and implementing a reaction, so that a composite material is obtained; and S5, adding the [beta]-CD and the composite material in the step S4 to a solution, implementing ultrasonic treatment, conducting a standing-by reaction at 4 DEG C for 12-24h, implementing cleaning and centrifuging, collecting a precipitate and drying the precipitate, so that the drug carrier is obtained. The drug carrier, which is prepared by the invention, can achieve targeted localization to an inflammation focus part in an external magnetic field; the drug carrier can be kept stable in human normal tissues and can achieve rapid release of drugs in the inflammation part; and response of the drugs to internal environment pH and controlled release are achieved.

Description

technical field [0001] The invention belongs to the technical field of biological material synthesis, and more specifically relates to a preparation method of a drug carrier with magnetic-pH double response. Background technique [0002] Based on the development of sustained and controlled release and targeted drug carriers, responsive preparations can release drugs in response to physiological and pathological changes in the human body, and are a new generation of intelligent drug carriers. The intelligence of drug delivery systems requires carrier materials to be environmentally sensitive, that is, responsive to external stimuli (such as magnetic fields, ultrasound, light, heat, etc.) or changes in the physiological environment (such as pH, temperature, redox environment, etc.). The introduction of dual intelligence factors enables drug carriers to respond to external stimuli and physiological environments simultaneously, which is one of the effective ways to develop respo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/61A61K47/02A61K31/7048A61P31/04
CPCA61K31/7048A61K47/02
Inventor 柯渔张晓晔黄晓君刘财坤
Owner JINAN UNIVERSITY