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Method for oriented production of low viscosity and ultra low viscosity algin through enzyme method based on ion regulation

An ultra-low viscosity, alginate technology, applied in chemical recovery, fermentation and other directions, can solve the problems of water consumption, molecular weight uniformity difference, etc., and achieve the effect of simple process, strong continuity and water saving.

Active Publication Date: 2017-10-03
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of serious water consumption and poor molecular weight uniformity in the traditional process, the present invention provides a method for producing low-viscosity and ultra-low-viscosity alginate by using alginate lyase

Method used

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  • Method for oriented production of low viscosity and ultra low viscosity algin through enzyme method based on ion regulation
  • Method for oriented production of low viscosity and ultra low viscosity algin through enzyme method based on ion regulation
  • Method for oriented production of low viscosity and ultra low viscosity algin through enzyme method based on ion regulation

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Embodiment 1

[0031] (1) Raw material pretreatment: Take 100 kg of dried kelp and soak it in 1000 L of water to remove impurities, and then fix the color in 100 L of 0.5% formaldehyde solution for 2 hours, then wash off the surface formaldehyde with water;

[0032] (2) Digestion: Digest the raw material in 2000 L of 6% sodium bicarbonate solution for 3.5 h at 60 °C with stirring;

[0033] (3) Enzyme activity regulation: KCl, NaCl and BaCl 2 The solution was added to the crude enzyme solution of alginate lyase, so that the final concentrations were 150 mmol / L, 500 mmol / L and 5 mmol / L respectively, and incubated at a constant temperature of 30 °C for 20 min, so as to achieve three levels of alginate lyase. Enzyme activity regulation of monomeric enzymes.

[0034] (4) Enzyme hydrolysis and filtration: Add 2000 L of water to the alkali-treated digestive solution for preliminary dilution, add 10% hydrochloric acid to adjust the pH to 6 after cooling to room temperature, and place them in membra...

Embodiment 2

[0040] (1) Raw material pretreatment: Soak 30 kg of dried macroalgae in 300 L of water for 3 hours to remove impurities, fix the color in 30 L of 0.5% formaldehyde solution for 4 hours, and wash off the surface formaldehyde with water;

[0041] (2) Digestion: Digest the raw material in 450 L of 6% sodium bicarbonate solution for 2 h at 65 °C with stirring;

[0042] (3) Enzyme activity regulation: KCl, NaCl and BaCl 2 The solution was added to the crude enzyme solution of alginate lyase, so that the final concentrations were 200 mmol / L, 450 mmol / L and 2.5 mmol / L, respectively, and incubated at a constant temperature of 30 °C for 30 min, so as to achieve the three levels of alginate lyase. Monomer enzymes regulate enzyme activity.

[0043] (4) Enzymolysis: Add 300 L of water to the alkali-treated digestion solution for preliminary dilution, add 10% hydrochloric acid to adjust the pH to 7 after cooling to room temperature, and place them in membrane bioreactors with a molecular ...

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Abstract

In order to solve the problems of serous water consumption, poor molecular weight uniformity and the like of the traditional process, the invention discloses a method for oriented production of low viscosity and ultra low viscosity algin through an enzyme method based on ion regulation. The method comprises: soaking phaeophyta, carrying out color fixing, digesting, transferring into a membrane bioreactor, adding algin lyases treated with a K<+>-Na<+>-Ba<2+> solution, carrying out enzymolysis-viscosity reducing, inactivating the enzyme, filtering to obtain a clear glue liquid, carrying out calcium precipitation on the clear glue liquid, removing the calcium, neutralizing, carrying out alcohol precipitation, drying, and carrying out other processes to obtain the low viscosity and ultra low viscosity algin. According to the present invention, the prepared algin product has good molecular weight uniformity, wherein the main component content is 80-100%; the method has advantages of simple process, strong continuity, low water consumption and no requirement of high temperature, high pressure and other harsh conditions, can overcome the problems of long enzyme treatment time and the like of other processes, can save water by 60-80% compared to the traditional production process so as to substantially save the water source.

Description

technical field [0001] The invention relates to a method for directional preparation of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation, specifically refers to using brown algae as raw material, using ions to regulate the activity of alginate lyase to degrade and produce low-viscosity and ultra-low-viscosity alginate Method of Viscosity Alginate. Background technique [0002] Alginate is a polysaccharide biopolymer extracted from brown algae such as kelp and sargassum, and is a linear polymer composed of mannuronic acid and guluronic acid. Compared with medium- and high-viscosity alginate, low-viscosity and ultra-low-viscosity alginate have the characteristics of small molecular weight, good stability, strong permeability, and good water solubility, and are widely used in printing and dyeing, medical and health and food industries. Low-viscosity and ultra-low-viscosity alginate occupies an important position in the world's alginate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/04C12P19/14Y02P20/582
Inventor 江晓路穆惠敏沈照鹏
Owner OCEAN UNIV OF CHINA
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