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A method for the directional preparation of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation

An ultra-low viscosity, alginate technology, applied in chemical recovery, fermentation and other directions, can solve the problems of water consumption, poor molecular weight uniformity, etc., and achieve the effect of simple process, strong continuity and cost saving.

Active Publication Date: 2021-03-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problems of serious water consumption and poor molecular weight uniformity in the traditional process, the present invention provides a method for producing low-viscosity and ultra-low-viscosity alginate by using alginate lyase

Method used

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  • A method for the directional preparation of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation
  • A method for the directional preparation of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation
  • A method for the directional preparation of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation

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Embodiment 1

[0031] (1) Raw material pretreatment: take 100kg of dried kelp and soak in 1000L of water to remove impurities, then fix the color in 100L of 0.5% formaldehyde solution for 2 hours, and then wash off the surface formaldehyde with water;

[0032] (2) Digestion: Digest the raw material in 2000L of 6% sodium bicarbonate solution for 3.5 hours at 60°C with stirring;

[0033] (3) Regulation of enzyme activity: KCl, NaCl and BaCl 2 Add the solution to the crude enzyme solution of alginate lyase so that the final concentrations are 150mmol / L, 500mmol / L and 5mmol / L respectively, and incubate at a constant temperature of 30°C for 20min, so as to realize the synthesis of the three monomeric enzymes of alginate lyase Enzyme activity regulation.

[0034] (4) Enzyme hydrolysis and filtration: Add 2000L of water to the alkali-treated digestive solution for preliminary dilution, add 10% hydrochloric acid after cooling to room temperature to adjust the pH to 6, and place them in membrane bio...

Embodiment 2

[0040] (1) Raw material pretreatment: Soak 30kg of dried macroalgae in 300L of water for 3 hours to remove impurities, fix the color in 30L of 0.5% formaldehyde solution for 4 hours, and wash off the surface formaldehyde with water;

[0041] (2) Digestion: Digest the raw material in 450L of 6% sodium bicarbonate solution for 2 hours at 65°C with stirring;

[0042] (3) Regulation of enzyme activity: KCl, NaCl and BaCl 2 Add the solution to the crude enzyme solution of alginate lyase so that the final concentrations are 200mmol / L, 450mmol / L and 2.5mmol / L respectively, and incubate at a constant temperature of 30°C for 30min, so as to realize the three monomeric enzymes of alginate lyase Control enzyme activity.

[0043] (4) Enzymolysis: Add 300L of water to the alkali-treated digestive solution for initial dilution, add 10% hydrochloric acid after cooling to room temperature to adjust the pH to 7, place them in membrane bioreactors with a molecular weight cut-off of 40kDa, and ...

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Abstract

In order to solve the problems of serious water consumption and poor molecular weight uniformity in the traditional process, the present invention discloses a method for directional production of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation. After the brown algae is soaked, solidified and digested , transferred to the membrane bioreactor and added via K + 、Na + and Ba 2+ The alginate lyase treated with the solution is used for enzymatic hydrolysis to reduce the viscosity, and after the end, the enzyme is inactivated and filtered to obtain clear glue. The clear glue solution is then subjected to processes such as calcium precipitation, decalcification, neutralization, alcohol precipitation, and drying to obtain low-viscosity and ultra-low-viscosity alginate. The alginate product prepared by the invention has good molecular weight uniformity, and the main component content is between 80-100%. The invention has the advantages of simple process, strong continuity, less water consumption, no need for harsh conditions such as high temperature and high pressure, and overcomes the problems of long enzymatic treatment time and the like in other processes. Compared with traditional process production, it can save 60-80% of water consumption, which greatly saves the consumption of water resources.

Description

technical field [0001] The invention relates to a method for directional preparation of low-viscosity and ultra-low-viscosity alginate by enzymatic method based on ion regulation, specifically refers to using brown algae as raw material, using ions to regulate the activity of alginate lyase to degrade and produce low-viscosity and ultra-low-viscosity alginate Method of Viscosity Alginate. Background technique [0002] Alginate is a polysaccharide biopolymer extracted from brown algae such as kelp and sargassum, and is a linear polymer composed of mannuronic acid and guluronic acid. Compared with medium- and high-viscosity alginate, low-viscosity and ultra-low-viscosity alginate have the characteristics of small molecular weight, good stability, strong permeability, and good water solubility, and are widely used in printing and dyeing, medical and health and food industries. Low-viscosity and ultra-low-viscosity alginate occupies an important position in the world's alginate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/04
CPCC12P19/04C12P19/14Y02P20/582
Inventor 江晓路穆惠敏沈照鹏
Owner OCEAN UNIV OF CHINA
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