Electrochemical sensor for detecting mercury ions

An electrochemical and sensor technology, applied in the field of biosensors, can solve the problems of high cost, cumbersome and time-consuming processing, and achieve the effects of fast reaction speed, improved sensitivity, and mild reaction conditions

Active Publication Date: 2017-10-03
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the commonly used methods for mercury determination mainly include cold atomic absorption method, cold atomic fluorescence method, chromatography and plasma inductively coupled mass spectrometry. Although these methods have high accuracy, they often require large-scale instruments and equipment, and the ...

Method used

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  • Electrochemical sensor for detecting mercury ions

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Embodiment 1

[0035] 1) The gold electrode is first polished in 0.3 μM and 0.05 μM alumina slurry until it becomes a mirror surface, and then rinsed repeatedly with PBS and secondary water;

[0036] 2) Add 10 μL of 10 μM capture probe dropwise to the electrode surface and incubate at room temperature for 2 hours; fix the sulfhydryl chain to the electrode surface through the Au-S bond;

[0037] 3) The main steps in a homogeneous reaction:

[0038] a. Add sterilized water, 10X buffer, 2μL exonuclease III, 2μL HAP with a concentration of 1μM and 2μL target substance (concentrations are 500nM, 200nM, 100nM, 1nM, 500pM, 100pM) to the pre-prepared In a sterilized centrifuge tube; shake for 30s, then put the mixed solution into a 30°C incubator and incubate for 50min;

[0039] b. Continue to add 2 μL 10 μM Padlock, 1 μL T4 ligase, 2 μL Phi29 polymerase and 2 μL dNTP to the centrifuge tube in step a; shake for 30 seconds, and then continue to incubate the mixed mixture in a 37°C incubator for 1 ho...

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Abstract

The present invention provides an electrochemical sensor for detecting mercury ions. According to the present invention, capture probe is fixed on the surface of an electrode through a Au-S bond, the surface of the electrode is modified with a reacted homogeneous phase solution, the RCA product in the homogeneous phase and the capture probe on the surface of the electrode are subjected to a hybridization reaction by incubating for 2 h at a temperature of 37 DEG C so as to fix the RCA product on the surface of the electrode, the obtained electrode is soaked for 10 min in a methylene blue solution, and the redox peak of MB is detected with a three-electrode working system. According to the present invention, the signal amplification is achieved through the target cycle amplification and the rolling ring amplification, such that the high-sensitivity mercury ion detection can be achieved, and the low detection lower limit can be obtained.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting divalent mercury ions. Background technique [0002] As a global environmental pollutant, mercury has teratogenic and carcinogenic effects, is an accumulative poison, and is non-biodegradable. Its artificial release and its impact on ecosystem functions and human health have attracted widespread attention from the international community. Mercury pollution mostly comes from solid waste incineration and fossil fuel combustion, which can exist in the atmosphere for a long time and have seriously polluted some water sources and soil. Moreover, inorganic mercury can be converted into more toxic methylmercury under the action of some microorganisms in the marine environment, and accumulate in all links of the food chain, and finally enter the human body through the food chain, and will be deposited in the brain, liver and other organs. Produce chronic poi...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/30G01N27/3275
Inventor 刘素冷雪琪王玉裴倩倩崔雪君韩聪徐成功刘学娇秦艺菲
Owner UNIV OF JINAN
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