Electrochemical sensor for detecting mercury ions

Abstract

The present invention provides an electrochemical sensor for detecting mercury ions. According to the present invention, capture probe is fixed on the surface of an electrode through a Au-S bond, the surface of the electrode is modified with a reacted homogeneous phase solution, the RCA product in the homogeneous phase and the capture probe on the surface of the electrode are subjected to a hybridization reaction by incubating for 2 h at a temperature of 37 DEG C so as to fix the RCA product on the surface of the electrode, the obtained electrode is soaked for 10 min in a methylene blue solution, and the redox peak of MB is detected with a three-electrode working system. According to the present invention, the signal amplification is achieved through the target cycle amplification and the rolling ring amplification, such that the high-sensitivity mercury ion detection can be achieved, and the low detection lower limit can be obtained.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting divalent mercury ions. Background technique [0002] As a global environmental pollutant, mercury has teratogenic and carcinogenic effects, is an accumulative poison, and is non-biodegradable. Its artificial release and its impact on ecosystem functions and human health have attracted widespread attention from the international community. Mercury pollution mostly comes from solid waste incineration and fossil fuel combustion, which can exist in the atmosphere for a long time and have seriously polluted some water sources and soil. Moreover, inorganic mercury can be converted into more toxic methylmercury under the action of some microorganisms in the marine environment, and accumulate in all links of the food chain, and finally enter the human body through the food chain, and will be deposited in the brain, liver and other organs. Produce chronic poi...

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used methods for mercury determination mainly include cold atomic absorption method, cold atomic fluorescence method, chromatography and plasma inductively coupled mass spectrometry. Although these methods have high accuracy, they often require large-scale instruments and equipment, and the cost is high. The process is cumbersome and time-consuming, and cannot meet the needs of high efficiency and low cost in actual monitoring

However, the existing mercury ion sensing technology is still in its infancy, and it is still a big challenge to develop a more sensitive, simpler and faster mercury ion detection technology.

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