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Preparation method and application of hypoglycemic Chinese hickory leaf total flavonoids

A technology of pecan leaves and total flavonoids is applied in the field of preparation of pecan leaves total flavonoids, which can solve problems such as many adverse reactions, and achieve the effects of reducing curative effect, stable quality and significant effect.

Inactive Publication Date: 2017-10-17
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In response to this situation, a large number of hypoglycemic drugs have come out. However, most of the common clinical diabetes treatment drugs are chemically synthesized drugs. Although the curative effect is definite, there are also many adverse reactions

Method used

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  • Preparation method and application of hypoglycemic Chinese hickory leaf total flavonoids
  • Preparation method and application of hypoglycemic Chinese hickory leaf total flavonoids
  • Preparation method and application of hypoglycemic Chinese hickory leaf total flavonoids

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Experimental program
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Effect test

Embodiment 1

[0020] After hickory leaves are dried, mechanically pulverize, take by weighing hickory leaf powder 200g, add 10 times of amount (g:mL) volume percentage is 95% ethanol solution, extract 2 times, each 30min, combine filtrate, at 300 ± Concentrate under reduced pressure under 100pa vacuum to make the ethanol concentration in the filtrate 65%, centrifuge at 3000 rpm for 20 minutes, and take the supernatant. Weigh 4.0 g of SD200 aromatic macroporous adsorption resin (Zhejiang Zhengguang Industrial Co., Ltd.), put it into a chromatographic column (15mm×350mm i.d), and load the sample at a flow rate of 2BV / h, with a sample volume of 6.5BV. First eluted with 10BV of water, then eluted with 10BV of 20% ethanol solution by volume percentage, and finally eluted with 10BV of 70% ethanol solution by volume percentage, the elution rate was 4BV / h, and the 70% ethanol eluate was collected , reduced pressure and rotary evaporation to dryness to obtain the total flavonoids of hickory nut leaf...

Embodiment 2

[0022] After the hickory leaves are dried, crush them mechanically, weigh 400g of hickory leaf powder, add 10 times the amount of ethanol solution with a volume percentage of 95%, extract twice, each time for 30min, combine the filtrates, and depressurize under a vacuum of 300±100pa Concentrate so that the ethanol concentration in the filtrate is 55%, centrifuge at 4000 rpm for 20 minutes, and take the supernatant. Weigh 200.0g of SD200 macroporous adsorption resin, put it into a chromatographic column (15mm×350mm i.d), elute with 10BV water, 20% ethanol, 40% ethanol, 70% ethanol in sequence, and collect the 70% ethanol eluate , evaporated to dryness under reduced pressure to obtain the total flavonoids extract of hickory nut leaves, wherein the total content of coneconol, coneconol-chalcone, chrysin and adzukimin is more than 52.2%.

Embodiment 3

[0024] After hickory leaves are dried, mechanically pulverize, take hickory leaf powder 200g, add 10 times of amount (g:mL) volume percent is 95% ethanol solution, extract 3 times, each 90min, combine filtrate, at 300 ± Concentrate under reduced pressure under 100pa vacuum to make the ethanol concentration in the filtrate 65%, centrifuge at 2000 rpm for 20 minutes, and take the supernatant. Weigh 4.0 g of SD200 aromatic macroporous adsorption resin (Zhejiang Zhengguang Industrial Co., Ltd.), put it into a chromatographic column (15mm×350mm i.d), and load the sample at a flow rate of 8BV / h, with a sample volume of 8BV. First eluted with 5BV water, then eluted with 12BV ethanol solution with a volume percentage of 30% at a flow rate of 1BV / h, and finally eluted with a 10BV ethanol solution with a volume percentage of 90% at a flow rate of 5BV / h, and collected 90% ethanol solution. Deliquification, rotary evaporation under reduced pressure to dryness, to obtain total flavonoids o...

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Abstract

The invention relates to a preparation method and application of hypoglycemic Chinese hickory leaf total flavonoids; the Chinese hickory leaf total flavonoids exhibit good hypoglycemic effect at all doses, have significant effect for diabetes mellitus, and are applicable to the preparation of drugs, health foods and the like for preventing or treating diabetes mellitus.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a preparation method and application of pecan leaf total flavonoids with hypoglycemic effect. Background technique [0002] Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia. Clinically, it mainly manifests as chronic hyperglycemia accompanied by carbohydrate, fat and protein metabolic disorders. The course of the disease is long, and if it is not treated in time and effectively, it is likely to cause damage to multiple organs throughout the body, infection, blindness, kidney disease and other complications, which will seriously endanger human health. Due to its wide range of patients and its increasing incidence in recent years, diabetes has become the third major disease that endangers human health after cardiovascular disease and cancer. In response to this situation, a large number of hypoglycemic drugs have come out. How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61P3/10A23L33/105A61K127/00
CPCA61K36/52A23L33/105A23V2002/00A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23V2200/328A23V2250/2116
Inventor 丁志山陆金健钱朝东黄燕芬高承贤陈建真
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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