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Morchella vulgaris, rhizoma polygonati and snowdrop active proteoglycan protein, as well as preparation method and application thereof

A technology of active polysaccharides and morels, applied in the preparation method of peptides, chemical instruments and methods, peptide/protein components, etc., can solve problems such as the lack of a unified understanding, and achieve the effect of improving activity

Active Publication Date: 2017-10-20
秦小波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, how to obtain polysaccharide proteins with important medicinal activities from some traditional Chinese medicines has not yet formed a unified understanding

Method used

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  • Morchella vulgaris, rhizoma polygonati and snowdrop active proteoglycan protein, as well as preparation method and application thereof
  • Morchella vulgaris, rhizoma polygonati and snowdrop active proteoglycan protein, as well as preparation method and application thereof
  • Morchella vulgaris, rhizoma polygonati and snowdrop active proteoglycan protein, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 10 g of Morchella, Rhizoma Rhizoma Polygonatum, and snow lotus dry materials and mix a total of 30 g, pulverize to 300 mesh with a superfine pulverizer, then add 0.1M sodium chloride solution with a mass-volume ratio of 15 times the volume, stir evenly, and extract 6 hours, filtered through two layers of gauze, and centrifuged (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0036] Take the above precipitate, add 0.5M sodium chloride solution with a mass volume ratio of 5 times the volume, stir evenly, extract for 2 hours, filter through two layers of gauze, and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0037] All the obtained supernatants were combined to obtain an extract.

[0038] Add sulfuric acid to the extract to reach 40% saturation, let it stand for 5 hours, and centrifuge (4°C, 10,000g, 20min) to remove the precipitate;

[0039] Take the supernatant, use ultrafiltration to concentrate and desalt, the mol...

Embodiment 2

[0042] Get 20g of hickory chick, sealwort dry material and mix altogether 40g, pulverize to 300 mesh with superfine pulverizer, then add the 0.3M sodium chloride solution of mass volume ratio 10 times of volume, stir well, leaching 6 hours, 2 Filter through a layer of gauze and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0043] Take the above precipitate, add 0.3M sodium chloride solution with a mass volume ratio of 8 times the volume, stir evenly, extract for 4 hours, filter through two layers of gauze, and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0044] All the obtained supernatants were combined to obtain an extract.

[0045] Add sulfuric acid to the extract to reach 40% saturation, leave it for 6 hours, and centrifuge (4°C, 10,000g, 20min) to remove the precipitate;

[0046] Take the supernatant, use ultrafiltration to concentrate and desalt, the molecular weight cut-off of the ultrafiltration membrane i...

Embodiment 3

[0049]Get 50g of hickory chick dry material, pulverize to 300 order with superfine pulverizer, then add the 0.2M sodium chloride solution of mass volume ratio 20 times of volume, stir, leaching 4 hours, 3 layers of gauze filter, centrifugal ( 4°C, 5,000g, 30min), to obtain supernatant and precipitate.

[0050] Take the above precipitate, add 0.2M sodium chloride solution with a mass volume ratio of 10 times the volume, stir evenly, extract for 3 hours, filter with 3 layers of gauze, and centrifuge (4°C, 5,000g, 30min) to obtain the supernatant and precipitate.

[0051] All the obtained supernatants were combined to obtain an extract.

[0052] Add sulfuric acid to the extract to reach 40% saturation, let it stand for 4 hours, and centrifuge (4°C, 10,000g, 20min) to remove the precipitate;

[0053] Take the supernatant, use ultrafiltration to concentrate and desalt, the molecular weight cut-off of the ultrafiltration membrane is 5000 Dalton; the volume is concentrated to 30% of...

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Abstract

The invention provides a method for preparing active proteoglycan protein. The method comprises the following steps: (1) performing superfine grinding on a raw material to 300 meshes, adding a sodium chloride solution to extract, filtering and centrifuging to obtain precipitate and supernate; (2) adding ammonium sulfate into the obtained supernate until the saturability is 40 percent, standing, centrifuging and removing the sediment; (3) performing ultrafiltration on the substrate obtained in the step (2) by utilizing an ultrafiltration membrane, and concentrating to 20-30 percent of the original volume; (4) adding alcohol into the substance obtained in the step (3), precipitating, and centrifuging to obtain precipitate; and (5) lyophilizing the substance obtained in the step (4) to obtain the active proteoglycan protein, wherein the raw material is at least one of morchella vulgaris, rhizoma polygonati and snowdrop. The active proteoglycan protein has high purity and relatively high yield. Compared with the prior art, the activity of the active proteoglycan protein for inhibiting oxygen radical, removing hydroxyl radical and inhibiting tumor cells can be greatly improved.

Description

technical field [0001] The invention specifically relates to fungi and plant active polysaccharide proteins such as hickory chick, polygonatum, snowdrop, etc., and a preparation method and application thereof. Background technique [0002] Carbohydrate-binding proteins are a class of binding proteins that have specific binding properties to various sugars. Most of them have hemagglutination activity, and some also have the effect of promoting cell mitosis. These properties are not significantly different from lectins, so some people call this type of protein a lectin, and some call it a receptor (receptors can be proteins, glycoproteins, glycolipids, or other compounds, so it should be said that they are multiple receptors one of the categories). For example, the galactose-specific binding protein on the mammalian liver cell membrane, Ashwell and other researchers also called it lectin and receptor in the same article, which are mainly named from different perspectives acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K14/415C07K14/37A61K38/16A61P35/00A61P31/12A61P37/04
CPCA61K38/16C07K1/303C07K1/34C07K1/36C07K14/37C07K14/415
Inventor 秦小波鲍锦库牛蓓张国珍李圆圆徐莺宁华吴传芳张树梅吴霞
Owner 秦小波
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