Infant's Oral Cell DNA Extraction Kit

Abstract

The invention discloses an oral cavity cell DNA (deoxyribonucleic acid) extracting kit for an infant. The kit comprises an oral cavity cell collecting swab, a heat preservation part and a packaging part; the oral cavity cell collecting swab and the heat preservation part are packaged in the packaging part; and the invention further relates to a method for extracting the oral cavity cell DNA by using the kit. The kit has the beneficial effects that the oral cavity cell collecting swab is safe, comfortable and efficient; after sampling, low temperature can last in the sending process to eradicate microbial contamination; the operation is simple and easy, and the exposure duration of the swab is reduced; the yield is high; at present, the oral cavity cell DNA extracting kit in the market can be used for extracting only 3 micrograms of genomes at most once, and if a large quantity of gene manipulation needs to be performed subsequently, multiple times of sampling extraction are required; the kit can obtain 50 micrograms of genomes under the condition of same sampling quantity; the kit is an oral cavity cell DNA extracting kit specific to the infant, gives consideration to various difficulties in infant sampling and amplification, and can be used for efficiently completing the steps such as sampling, preservation and extraction.

Description

technical field [0001] The invention relates to a DNA extraction kit for oral cavity cells of infants. Background technique [0002] With the sharp increase in the demand for infant gene preservation, susceptibility gene detection and prenatal diagnosis in recent years, the demand for oral cell DNA extraction kits is also increasing year by year. [0003] However, the buccal cell DNA extraction kits currently on the market have the following significant defects: (1) the sampling swab is crude, usually using a rough cotton swab or a rough chemical fiber scraper, the sampling efficiency is not high, and it is difficult to judge the sampling effect. (2) After the swab is sampled, it is easy to be corrupted and deteriorated during the process of sending it to the testing unit, and subsequent operations cannot be performed. (3) The current kits often require the swabs to be dried in the air in order to prevent the swabs from spoiling. This step is very easy to cause sample conta...

AI Technical Summary

Problems solved by technology

[0003] However, the buccal cell DNA extraction kits currently on the market have the following significant defects: (1) The sampling swab is crude, usually using a rough cotton swab or a rough chemical fiber scraper, the sampling efficiency is not high, and it is difficult to judge the sampling effect

(2) After the swab is sampled, it is easy to be corrupted and deteriorated during the process of sending it to the testing unit, and it is impossible to perform follow-up operations

(3) The current kits often require the swabs to be dried in the air to prevent the swabs from spoilage. This step can easily cause sample contamination

(4) At present, oral cell DNA extraction kits on the market can only extract 3ug of genome from one sampling at most.

(5) At present, there is no oral DNA extraction kit specifically for infants on the market, because it is difficult to sample oral cells of infants, and the sampling volume is extremely small. amount of gene required

Although the technical solution has made some breakthroughs, it has not solved the problems of low sampling efficiency, low yield and complicated operation.

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