Preservation method of excrement sample, solution for preservation, preparation method and application

Abstract

The invention relates to a preservation method of an excrement sample, a solution for preservation, a preparation method and application. A corresponding excrement preservation solution can be prepared from materials according to a formula, without the need of complicated operation; collected excrement is directly put into the preservation solution and can reach the effect of stabilizing microorganism components, without the need of special treatment. The collected excrement is mixed with the preservation solution which takes a water-soluble organic solvent as an effective component to prepare the excrement sample which can keep an excellent preservation property of microorganisms in the excrement sample. When the excrement sample is preserved by utilizing the formula, a frozen preservation effect of a sample can be realized under normal temperature, so that sample collection and preservation of the sample can be carried out at any time and long-time transportation of the excrement sample is convenient to realize. The excrement preservation solution provided by the invention is safe and efficient, can be used for maintaining the abundance of bacteria in the excrement for long time, is low in cost and has a good application prospect and economic benefits.

Description

technical field [0001] The invention relates to a preservation method, a preservation solution, a preparation method and an application of a feces sample, and belongs to the field of biotechnology. Background technique [0002] Human microorganisms are an organic part of the human body, with a large number, accounting for about 1% to 2% of the total body weight in dry weight, and various types (including bacteria, archaea, fungi and viruses, etc.), colonized in the gastrointestinal tract, oral cavity, skin, etc. , genitourinary tract, etc., the number of encoded genes is 50 to 100 times that of the human body's own genes, which is equivalent to the "second genome" of the human body. There are 100 trillion bacteria on the human body, weighing about 3 catties. [0003] We live in an environment surrounded by bacteria and deal with them every day. There are more than 1,000 species of normal flora in the human body, and the number is 100 trillion (human cells are only 10 trill...

AI Technical Summary

Problems solved by technology

However, effective preservation of the freshness of the original sample becomes impossible in situations such as out-of-patient visits or the impossibility of rapid cooling after faecal collection at the customer's home

[0015] In addition, in order to prevent the deterioration of the stool sample, it is considered to freeze the stool sample, but the frozen stool sample must be thawed before the test, so the operation becomes complicated

In the conventional method, by adding a fungicide, etc., it is possible to prepare and store the stool sample at room temperature without cooling operation, but there is a problem that the operation of isolating the bacteria exfoliated from the large intestine from the stool is complicated.

In addition, nucleic acid decomposing enzymes and proteolytic enzymes derived from bacteria, which are destroyed by bactericides, etc., will degrade nucleic acids, etc.

[0016] However, there is no similar room temperature preservation technology at home and abroad. Therefore, it is imminent to invent a stool-like bacterial preservation solution to solve the problems of normal temperature transportation and preservation of bacteria-containing samples such as feces and intestinal contents and the solidification of bacterial flora. The invention and preparation can effectively solve the problems of normal temperature transportation and preservation of bacteria-containing samples and the change of bacterial richness

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