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Pharmaceutical composition with anticancer effect

A technology of anti-cancer drugs and compositions, which is applied in the field of medicine and can solve the problems of large differences in anti-tumor activity

Inactive Publication Date: 2017-11-10
黄娇艳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, different monomers in such substances have very different antitumor activities, and the same monomers have very large differences in the inhibitory activities of different tumors. At present, there is no such substance monomer or composition as a marketed product. Anti-cancer drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Anti-liver cancer activity of pharmaceutical composition

[0022] Human liver cancer cells BEL-7404 were cultured in culture medium (RPMI 1640 medium). 3 Add each well to a 96-well plate, culture in an incubator containing 5% CO2 at 37°C for 24 hours, and then absorb the original medium after attaching to the wall. Then the blank group was added RPMI 1640 medium containing 10% fetal bovine serum; the drug treatment group was added RPMI 1640 medium containing 0.01-100 μM drug to be tested; the positive control group was added RPMI 1640 medium containing 0.01-100 μM cisplatin; After continuing to cultivate for 48 h, add MTT at a concentration of 5 mg / mL, continue to culture for 4 h, suck off the supernatant, add 100 μL DMSO, and place in the dark for 10 min. Measure the absorbance at 570 nm with a microplate reader (Sunrise Company). The cell viability was calculated according to the absorbance value, and 6 replicate wells were set for each treatment. Cell sur...

Embodiment 2

[0029] Example 2 Anti-lung cancer activity of pharmaceutical composition

[0030] Human lung cancer cells A549 were cultured in culture medium (RPMI 1640 medium), and the lung cancer cells A549 were cultured at 10×10 3 Add each well to a 96-well plate, culture in an incubator containing 5% CO2 at 37°C for 24 hours, and then absorb the original medium after attaching to the wall. Then the blank group was added RPMI 1640 medium containing 10% fetal bovine serum; the drug treatment group was added RPMI 1640 medium containing 0.01-100 μM drug to be tested; the positive control group was added RPMI 1640 medium containing 0.01-100 μM cisplatin; After continuing to culture for 48 h, add MTT at a concentration of 5 mg / mL, continue to culture for 4 h, suck off the supernatant, add 100 μL of DMSO, place in the dark for 10 min, and use a microplate reader (Sunrise Company) to measure the absorbance at 570 nm , and calculate the cell viability according to the absorbance value, and set 6...

Embodiment 3

[0037] Example 3 Anti-nasopharyngeal Carcinoma Activity of Pharmaceutical Composition

[0038] Human nasopharyngeal carcinoma cell CNE was cultured in culture medium (RPMI 1640 medium), and nasopharyngeal carcinoma cell CNE was cultured at 10×10 3 Add each well to a 96-well plate, culture in an incubator containing 5% CO2 at 37°C for 24 hours, and then absorb the original medium after attaching to the wall. Then the blank group was added RPMI 1640 medium containing 10% fetal bovine serum; the drug treatment group was added RPMI 1640 medium containing 0.01-100 μM drug to be tested; the positive control group was added RPMI 1640 medium containing 0.01-100 μM cisplatin; After continuing to culture for 48 h, add MTT at a concentration of 5 mg / mL, continue to culture for 4 h, suck off the supernatant, add 100 μL of DMSO, place in the dark for 10 min, and use a microplate reader (Sunrise Company) to measure the absorbance at 570 nm , and calculate the cell viability according to th...

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PUM

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Abstract

The invention relates to the field of medicines, and specifically discloses a pharmaceutical composition with anticancer effect. The pharmaceutical composition comprises the following active ingredients: resibufogenin, cinobufagin and bufotalin; wherein the weight ratio of resibufogenin, cinobufagin and bufotalin is 1-2:1-5:1-3. Tests show that after combination of the resibufogenin, the cinobufagin and the bufotalin, a synergistic anti liver cancer, lung cancer and nasopharyngeal carcinoma effect can be played, when the weight ratio of resibufogenin, cinobufagin and bufotalin is 1:1.5-2.5:1-2, the synergistic anti liver cancer effect is best, the anti liver and lung cancer effect of the pharmaceutical composition is equivalent to that of positive control drug cisplatin, and the anti nasopharyngeal carcinoma effect of the pharmaceutical composition better than that of the positive control drug cisplatin, so that the resibufogenin, the cinobufagin and the bufotalin can be combinedly used as a new anticancer drug.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a medicine composition with anticancer effect. Background technique [0002] Toad venom is the secretion of toad's post-auricular glands and epidermal glands, white milky liquid or light yellow serous, poisonous. Toad venom is extracted from the slurry, dried, colored, and can be used as medicine after being sealed and stored. It has many functions such as detoxification, analgesia, resuscitation, and antitumor, and is widely used. Germany has used toad venom preparations in the clinical treatment of coronary heart disease, and Japan has used toad venom as raw materials to produce "life-saving pills". There are more than 50 kinds of Chinese patent medicines such as Meihua Dianyu Dan, Yili Yatong Pill, Xinbao, and Cinobufacin Injection, all of which contain Toad Su. [0003] However, the existing marketed drugs mainly use toad venom extract as the active ingredient, and the active ingr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61P35/00
CPCA61K31/585A61K2300/00
Inventor 黄娇艳
Owner 黄娇艳
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