Pharmaceutical composition with anticancer effect
A technology of anti-cancer drugs and compositions, which is applied in the field of medicine and can solve the problems of large differences in anti-tumor activity
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Embodiment 1
[0021] Example 1 Anti-liver cancer activity of pharmaceutical composition
[0022] Human liver cancer cells BEL-7404 were cultured in culture medium (RPMI 1640 medium). 3 Add each well to a 96-well plate, culture in an incubator containing 5% CO2 at 37°C for 24 hours, and then absorb the original medium after attaching to the wall. Then the blank group was added RPMI 1640 medium containing 10% fetal bovine serum; the drug treatment group was added RPMI 1640 medium containing 0.01-100 μM drug to be tested; the positive control group was added RPMI 1640 medium containing 0.01-100 μM cisplatin; After continuing to cultivate for 48 h, add MTT at a concentration of 5 mg / mL, continue to culture for 4 h, suck off the supernatant, add 100 μL DMSO, and place in the dark for 10 min. Measure the absorbance at 570 nm with a microplate reader (Sunrise Company). The cell viability was calculated according to the absorbance value, and 6 replicate wells were set for each treatment. Cell sur...
Embodiment 2
[0029] Example 2 Anti-lung cancer activity of pharmaceutical composition
[0030] Human lung cancer cells A549 were cultured in culture medium (RPMI 1640 medium), and the lung cancer cells A549 were cultured at 10×10 3 Add each well to a 96-well plate, culture in an incubator containing 5% CO2 at 37°C for 24 hours, and then absorb the original medium after attaching to the wall. Then the blank group was added RPMI 1640 medium containing 10% fetal bovine serum; the drug treatment group was added RPMI 1640 medium containing 0.01-100 μM drug to be tested; the positive control group was added RPMI 1640 medium containing 0.01-100 μM cisplatin; After continuing to culture for 48 h, add MTT at a concentration of 5 mg / mL, continue to culture for 4 h, suck off the supernatant, add 100 μL of DMSO, place in the dark for 10 min, and use a microplate reader (Sunrise Company) to measure the absorbance at 570 nm , and calculate the cell viability according to the absorbance value, and set 6...
Embodiment 3
[0037] Example 3 Anti-nasopharyngeal Carcinoma Activity of Pharmaceutical Composition
[0038] Human nasopharyngeal carcinoma cell CNE was cultured in culture medium (RPMI 1640 medium), and nasopharyngeal carcinoma cell CNE was cultured at 10×10 3 Add each well to a 96-well plate, culture in an incubator containing 5% CO2 at 37°C for 24 hours, and then absorb the original medium after attaching to the wall. Then the blank group was added RPMI 1640 medium containing 10% fetal bovine serum; the drug treatment group was added RPMI 1640 medium containing 0.01-100 μM drug to be tested; the positive control group was added RPMI 1640 medium containing 0.01-100 μM cisplatin; After continuing to culture for 48 h, add MTT at a concentration of 5 mg / mL, continue to culture for 4 h, suck off the supernatant, add 100 μL of DMSO, place in the dark for 10 min, and use a microplate reader (Sunrise Company) to measure the absorbance at 570 nm , and calculate the cell viability according to th...
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