Polyketone compound, preparation method and uses thereof

A polyketone compound and compound technology, applied in biochemical equipment and methods, organic chemical methods, microorganism-based methods, etc., can solve problems such as drugs that have not yet been seen, and achieve environmental pollution-free, simple operation process, and production cycle. short effect

Active Publication Date: 2019-10-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the chemical structure of the compound and its anticancer activity, so there are no related drugs on the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A polyketide compound structural formula is shown in I:

[0019] (I).

Embodiment 2

[0021] The preparation method of the polyketide compound shown in I formula, step is as follows:

[0022] (1) Fermentation production: the mangrove bacterium HK-22 with the preservation number CGMCC No.17574 was streaked and revived, and then a single colony was picked and inoculated into ISP 4 In the liquid culture medium, under the conditions of 28°C and 150 rpm / min, cultured for 3 days as the seed liquid; then the seed liquid was inoculated into the ISP at a volume ratio of 1:40 4 In the liquid culture medium, at 28 ℃, shaker fermentation 8 days, obtain the fermented liquid, wherein the above-mentioned ISP 4 The formula of the liquid medium is as follows: soluble starch 10 g, CaCO 3 2 g, K 2 HPO 4 1 g, MgSO 4 •7H 2 O 1 g, NaCl 1 g, FeSO 4 •7H 2 O 0.001 g, MnCl 2 •7H 2 O 0.001g, (NH 4 ) 2 SO 4 2 g, sea salt 10 g and purified water 1 L; mangrove bacteria, taxonomically named Riveria Hahella sp., the bacterium is HK-22 strain, which was preserved in the General...

Embodiment 3

[0030] In vitro anticancer activity test (MTT method)

[0031] (1) Experimental samples

[0032] Preparation of the test sample solution: the test sample is the pure product of Compound I isolated and purified in the above-mentioned Example 2, and an appropriate amount of sample is accurately weighed for testing the activity;

[0033] (2) Experimental method

[0034] In this experiment, the MTT method was used to test the in vitro cytotoxic activity of the compound. The tested tumor cell lines were human leukemia cell lines CCRF-CEM and K562. The positive control drug was Chidamide. The cells used in the experiment were adherent cells from passage 2 to passage 20 or evenly dispersed suspension cells, both of which had a cell density of about 60%. After taking the cells required for the experiment, inoculate them in a 96-well plate, and fully pipette into a single cell suspension. The number of cells is diluted to 10,000 / mL, 200 per well. µ L, at 37°C, 5% CO 2 Cultivate in ...

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PUM

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Abstract

The invention discloses a polyketone compound, a preparation method and uses thereof, wherein the polyketone compound has a structure formula represented by I. The preparation method comprises: obtaining a fermentation product containing a polyketone compound by carrying out fermentation culture on Hahellasp. HK-22 having a preservation number of CGMCC No.17574, extracting with ethyl acetate, andcarrying out evaporating drying to obtain a crude extract; and separating and purifying the crude extract through pressure reducing silica gel column chromatography, gel column chromatography, mediumpressure column chromatography and reverse phase semi-preparative high performance liquid chromatography to obtain the product. According to the present invention, the polyketone compound has anticancer effect, and can be used as a new drug component for inhibiting the proliferation of cancer cells.

Description

technical field [0001] The present invention relates to a polyketide compound, in particular to a polyketide compound and its preparation method and application. Background technique [0002] The ocean covers 70% of the earth. Due to the high salinity and anoxic environment, many macroscopic and microscopic organisms bred have unique metabolic pathways and survival and reproduction methods, resulting in a series of secondary species with specific activities. produce metabolites to ensure survival in diverse and harsh habitats. Mangroves refer to trees or shrubs that can grow in salty waters of tropical and subtropical coasts around the world. Due to long-term submersion in seawater, the organisms living here not only have species diversity, but also have more novel and complex metabolites. sex. Hahella sp. is a marine endemic bacterium, which can produce polyketides due to the expression of PKS synthase. Polyketides possess physiological and biological activities such as ...

Claims

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Application Information

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IPC IPC(8): C07C233/32A61K31/16A61P35/00A61P35/02C12P13/02C12N1/20C12R1/01
CPCC07C233/32A61P35/00A61P35/02C12P13/02C07B2200/07C12R2001/01C12N1/205
Inventor 斯拉瓦·爱泼斯坦何山丁立建粟登权原野张为艳贺小平严小军
Owner NINGBO UNIV
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