Polyketone compound, preparation method and uses thereof
A polyketone compound and compound technology, applied in biochemical equipment and methods, organic chemical methods, microorganism-based methods, etc., can solve problems such as drugs that have not yet been seen, and achieve environmental pollution-free, simple operation process, and production cycle. short effect
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Embodiment 2
[0021] The preparation method of the polyketide compound shown in I formula, step is as follows:
[0022] (1) Fermentation production: the mangrove bacterium HK-22 with the preservation number CGMCC No.17574 was streaked and revived, and then a single colony was picked and inoculated into ISP 4 In the liquid culture medium, under the conditions of 28°C and 150 rpm / min, cultured for 3 days as the seed liquid; then the seed liquid was inoculated into the ISP at a volume ratio of 1:40 4 In the liquid culture medium, at 28 ℃, shaker fermentation 8 days, obtain the fermented liquid, wherein the above-mentioned ISP 4 The formula of the liquid medium is as follows: soluble starch 10 g, CaCO 3 2 g, K 2 HPO 4 1 g, MgSO 4 •7H 2 O 1 g, NaCl 1 g, FeSO 4 •7H 2 O 0.001 g, MnCl 2 •7H 2 O 0.001g, (NH 4 ) 2 SO 4 2 g, sea salt 10 g and purified water 1 L; mangrove bacteria, taxonomically named Riveria Hahella sp., the bacterium is HK-22 strain, which was preserved in the General...
Embodiment 3
[0030] In vitro anticancer activity test (MTT method)
[0031] (1) Experimental samples
[0032] Preparation of the test sample solution: the test sample is the pure product of Compound I isolated and purified in the above-mentioned Example 2, and an appropriate amount of sample is accurately weighed for testing the activity;
[0033] (2) Experimental method
[0034] In this experiment, the MTT method was used to test the in vitro cytotoxic activity of the compound. The tested tumor cell lines were human leukemia cell lines CCRF-CEM and K562. The positive control drug was Chidamide. The cells used in the experiment were adherent cells from passage 2 to passage 20 or evenly dispersed suspension cells, both of which had a cell density of about 60%. After taking the cells required for the experiment, inoculate them in a 96-well plate, and fully pipette into a single cell suspension. The number of cells is diluted to 10,000 / mL, 200 per well. µ L, at 37°C, 5% CO 2 Cultivate in ...
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