Lung cancer treatment bufalin liposome preparation and preparation method thereof

The technology of liposome preparation and bufalin, which is applied in the field of traditional Chinese medicine, can solve the problems of low curative effect, unsatisfactory curative effect and large toxic and side effects of cancer, and achieve the effect of improving clinical curative effect.

Inactive Publication Date: 2013-12-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapeutic drugs have a certain effect on cancer, but they have severe side effects

Method used

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  • Lung cancer treatment bufalin liposome preparation and preparation method thereof
  • Lung cancer treatment bufalin liposome preparation and preparation method thereof
  • Lung cancer treatment bufalin liposome preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A bufalin liposome preparation for the treatment of lung cancer is made from bufatoxin, and the preparation method of the liposome is as follows:

[0026] (1) The ratio of raw materials for preparing liposomes is: lecithin 900mg, amphipathic polyethylene glycol 2000-distearoylphosphatidylethanolamine 90mg, cholesterol 90mg;

[0027] (2) The preparation method of bufalin liposome is prepared by ethanol injection method: take 90ml of PBS buffer solution with pH 7.4 in an eggplant-shaped bottle, and preheat it in a constant temperature water bath at 50°C for 35min;

[0028] (3) Take the liposome proportioned in step 1 into a beaker, add 36mg of bufolin, and then add an appropriate amount of absolute ethanol to dissolve it;

[0029] (4) After bufalin is completely dissolved, quickly inject it into the eggplant-shaped bottle with buffer solution preheated at constant temperature in step 2 to obtain a milky white liposome suspension, continue stirring for 1 hour, r...

Embodiment 2

[0030] Embodiment 2: A kind of bufalin liposome preparation for the treatment of lung cancer, made of bufalin, the preparation method of its liposome is as follows:

[0031] (1) The ratio of raw materials for preparing liposomes is: lecithin 600mg, amphiphilic polyethylene glycol 2000-distearoylphosphatidylethanolamine 60mg, cholesterol 60mg;

[0032] (2) The preparation method of bufalin liposome is prepared by ethanol injection method: take 90ml of PBS buffer solution with pH 7.4 in an eggplant-shaped bottle, and preheat it in a constant temperature water bath at 55°C for 30min;

[0033] (3) Take the liposome proportioned in step 1 in a beaker, add 24mg of bufalin, and then add an appropriate amount of absolute ethanol to dissolve it;

[0034] (4) After the bufalin is completely dissolved, quickly inject it into the eggplant-shaped bottle with a buffer solution preheated at a constant temperature in step 2 to obtain a milky white liposome suspension, continue to stir and hyd...

Embodiment 3

[0035] Example 3: Inhibitory effect of a bufalin liposome preparation on transplanted non-small cell lung cancer in nude mice

[0036] 1. Experimental animals and tumor strains

[0037] BALB / C-nu nude mice, 50, male and female, 4-6 weeks old, weighing 18-20 g, were purchased from the Animal Experiment Center of our school. Human lung cancer A549 cells were purchased from the Animal Experiment Center of Jiangsu Cancer Institute. Lung cancer A549 cells

[0038] 2. Modeling and experimental methods

[0039] Of 50 BALB / C-nu nude mice, 10 were randomly selected as the negative control group, and the remaining 40 were used for modeling. Take human lung cancer A549 cells in the logarithmic growth phase cultured in vitro, and adjust the cell concentration to 1×10 7 / ml. Under sterile conditions, human lung cancer A549 cells were inoculated on the back of BALB / C-nu nude mice with an inoculation volume of 0.2ml / mouse (the number of cells was 2×10 6 / only). The entire inoculation ...

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Abstract

The present invention discloses a lung cancer (non-small-cell lung carcinoma and small cell lung cancer) treatment bufalin liposome preparation and a preparation method thereof, wherein the lung cancer treatment bufalin liposome preparation is a liposome preparation prepared from a lung cancer treatment traditional Chinese medicine active effective component bufalin. The preparation method comprises: adopting an ethanol injection method to prepare liposomes to obtain the lung cancer treatment bufalin liposome preparation. According to the bufalin liposome preparation, in vivo animal anti-cancer pharmacological experiment results show that: significant growth inhibition effects are provided for lung cancers (non-small-cell lung carcinoma and small cell lung cancer), and anti-cancer activity of the lung cancer treatment bufalin liposome preparation is significantly higher than anti-cancer activity of the chemotherapy drug paclitaxel. In addition, the bufalin liposome preparation provides a higher anti-cancer effect than the bufalin non-liposome preparation.

Description

1. Technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a bufalin liposome preparation for treating lung cancer (non-small cell lung cancer and small cell lung cancer) and a preparation method thereof. 2. Background technology [0002] Cancer is a formidable enemy that endangers human health. There are about 1.6 million new cases of cancer in my country every year, and about 1.3 million people die of cancer every year. The number of deaths from cancer in the country is increasing year by year. my country is a high-incidence area for lung cancer, lung cancer and other cancers, and the cancer mortality rate remains high. . [0003] There are currently no effective drugs that cure most cancer patients. Although chemotherapeutic drugs have certain curative effects on cancer, they have severe side effects, and Chinese patent medicines generally have low curative effects on cancer, and the curative effects are not i...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/585A61P11/00A61P35/00
Inventor 章永红章迅
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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