Preparation method of anti-freeze agent for marine products

A technology of antifreeze and aquatic products, applied in the direction of food ingredients as antifreeze, food ingredients, food ingredients containing oligosaccharides, etc., can solve the problems of high sweetness and calories, affecting product flavor and nutritional value, and achieve sweetness Low taste and heat, avoid open chain denaturation, and enhance stability

Inactive Publication Date: 2017-11-21
江苏华友装饰工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the aquatic product processing industry, a mixture of sorbitol and sucrose is often used as an antifreeze agent to inhibit the freezing denaturation of aq

Method used

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  • Preparation method of anti-freeze agent for marine products

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0021] Example 1

[0022] First, rinse the red algae water and put it into a beater for beating, then add 2.8L deionized water, mix well, put it in a flash extractor, extract at 55℃ for 2min, then transfer it to a centrifuge at 6000r / Centrifuge for 15 minutes and collect the extract and precipitate; extract the precipitate twice, collect and combine the supernatants, place the combined supernatants in a flash concentrator, and concentrate to 8% of the original volume to obtain a concentrate , Then add the concentrated solution to 600mL of absolute ethanol, stir at 300r / min for 1h, stand for 20h, transfer to a centrifuge, centrifuge at 6000r / min for 15min, collect the precipitate to obtain the crude compound polysaccharide; weigh 1g crude product The compound polysaccharide was added to 20mL of deionized water, stirred at 300r / min for 10min, then added 10mL of isopropanol, stirred at 300r / min for 10min, then added 35mL of 20% sodium hydroxide solution, continue to stir the reacti...

Example Embodiment

[0023] Example 2

[0024] First, take the sargassum water, wash it, put it into a beater, add 3.0L of deionized water, mix well, place it in a flash extractor, extract at 55°C for 2 minutes, and then transfer it to a centrifuge at 7000r / Centrifuge for 18 minutes and collect the extract and precipitate; extract the precipitate twice, collect and combine the supernatants, place the combined supernatants in a flash concentrator, and concentrate to 9% of the original volume to obtain a concentrated solution , Then add the concentrated solution to 700mL of absolute ethanol, stir at 300r / min for 1h, stand for 22h, transfer to a centrifuge, centrifuge at 7000r / min for 18min, collect the precipitate to obtain the crude compound polysaccharide; weigh 1g crude product The compound polysaccharide was added to 30mL of deionized water, stirred at 350r / min for 15min, then added 11mL of isopropanol, stirred at 350r / min for 15min, and then added 40mL of 20% sodium hydroxide solution, continue t...

Example Embodiment

[0025] Example 3

[0026] First take the spirulina water, wash it, put it into a beating machine, and then add 3.0L deionized water, mix well, put it in a flash extractor, extract at 60℃ for 3min, then transfer it to a centrifuge at 8000r / Centrifuge for 20 minutes and collect the extract and precipitate; extract the precipitate three more times, collect and combine the supernatants, place the combined supernatants in a flash concentrator, and concentrate to 10% of the original volume to obtain a concentrate , Then add the concentrated solution to 800mL of absolute ethanol, stir at 400r / min for 2h, stand for 24h, transfer to a centrifuge, centrifuge at 8000r / min for 20min, collect the precipitate to obtain the crude compound polysaccharide; weigh 2g of crude product The compound polysaccharide was added to 40mL of deionized water, stirred at 400r / min for 20min, then added 12mL of isopropanol, stirred at 400r / min for 20min, then added 42mL of 20% sodium hydroxide solution, continu...

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Abstract

The invention relates to a preparation method of an anti-freeze agent for marine products, and belongs to the technical field of marine product processing. According to the preparation method of the anti-freeze agent for marine products, carboxymethylation is conducted on protein and polysaccharide in a crude product complex polysaccharide, and combination of abundant inherent hydroxyl groups with some groups of protein molecules enables the protein molecules to be in a saturated state, thereby avoiding aggregation and denaturation of the protein molecules. Simultaneously, by the hydrogen-bond interaction of carboxymethyl groups stronger than that of the hydroxyl groups, the state of bound water embedded in the protein molecules is changed, and the bound water on the surfaces of the protein molecules is substituted by the carboxymethyl groups to be bound with the protein molecules, thereby playing a role of inhibiting denaturation of protein. Simultaneously, by enhancing the stability of water with the protein in the freezing process, formation of large ice crystals is reduced, thereby avoiding open-chain denaturation caused by spatial conformation change of fish protein, and effectively inhibiting freeze denaturation of the protein in the marine products. The anti-freeze agent has low sweetness and calorific value and has no influence on the flavor and nutritional values of the marine products.

Description

technical field [0001] The invention relates to a preparation method of an antifreeze agent for aquatic products, belonging to the technical field of aquatic product processing. Background technique [0002] Aquatic products are delicious, nutritious, and have strong health effects. However, because the water content of fresh aquatic products is about 70%, the tissues are fragile, the natural immune substances are less, the unsaturated fatty acids are easily oxidized, and the soluble protein content is high, so they are better than ordinary animals. Meat tissue is more prone to spoilage. At present, the most commonly used storage method is low-temperature freezing storage, which can effectively inhibit the reproduction of microorganisms, and at the same time reduce the biochemical reaction rate inside the material protein, thereby prolonging the shelf life of aquatic products, but long-term low-temperature freezing will lead to muscle growth in fish. Fibrillar protein denat...

Claims

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Application Information

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IPC IPC(8): A23B4/20
CPCA23B4/20A23V2002/00A23V2200/206A23V2250/51A23V2250/6414A23V2250/28
Inventor 吴义峰王之霖
Owner 江苏华友装饰工程有限公司
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