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Preparation method of anti-freeze agent for marine products

A technology of antifreeze and aquatic products, applied in the direction of food ingredients as antifreeze, food ingredients, food ingredients containing oligosaccharides, etc., can solve the problems of high sweetness and calories, affecting product flavor and nutritional value, and achieve sweetness Low taste and heat, avoid open chain denaturation, and enhance stability

Inactive Publication Date: 2017-11-21
江苏华友装饰工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the aquatic product processing industry, a mixture of sorbitol and sucrose is often used as an antifreeze agent to inhibit the freezing denaturation of aquatic product proteins, but its sweetness and calories are relatively high, which affects the flavor and nutritional value of the product to a certain extent

Method used

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  • Preparation method of anti-freeze agent for marine products

Examples

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example 1

[0022] First, wash the red algae with water and put them into a beater for beating, then add 2.8L of deionized water, mix them evenly, place them in a flash extractor, extract at 55°C for 2min, and then transfer them to a centrifuge at 6000r / Centrifuge for 15 min, collect the extract and precipitate; repeat the extraction of the precipitate twice, collect and combine the supernatant, put the combined supernatant in a flash concentrator, and concentrate to 8% of the volume of the original solution to obtain a concentrated solution , then add the concentrated solution into 600mL of absolute ethanol, stir at 300r / min for 1h, let it stand for 20h, transfer it to a centrifuge, and centrifuge at 6000r / min for 15min, collect the precipitate to obtain the crude compound polysaccharide; weigh 1g of the crude product Add the complex polysaccharide to 20mL of deionized water, stir at 300r / min for 10min, then add 10mL of isopropanol, stir at 300r / min for 10min, then add 35mL of 20% sodium...

example 2

[0024] First, wash the sargassum algae and put them into a beater for beating, then add 3.0L of deionized water, mix well, place in a flash extractor, extract at 55°C for 2min, and then transfer to a centrifuge for 7000r / Centrifuge for 18 min, collect the extract and precipitate; repeat the extraction of the precipitate twice, collect and combine the supernatant, put the combined supernatant in a flash concentrator, and concentrate to 9% of the volume of the original solution to obtain a concentrated solution , then add the concentrated solution into 700mL of absolute ethanol, stir at 300r / min for 1h, let it stand for 22h, transfer it to a centrifuge, and centrifuge at 7000r / min for 18min, collect the precipitate to obtain the crude compound polysaccharide; weigh 1g of the crude product Add the complex polysaccharide to 30mL of deionized water, stir at 350r / min for 15min, then add 11mL of isopropanol, stir at 350r / min for 15min, then add 40mL of 20% sodium hydroxide solution, ...

example 3

[0026] First, wash the spirulina with water and put it into a beater for beating, then add 3.0L deionized water, mix evenly, place it in a flash extractor, extract at 60°C for 3min, and then transfer it to a centrifuge for 8000r / Centrifuge for 20 min, collect the extract and precipitate; repeat the extraction of the precipitate 3 times, collect and combine the supernatant, put the combined supernatant in a flash concentrator, concentrate to 10% of the volume of the original solution, and obtain a concentrated solution , then add the concentrated solution into 800mL of absolute ethanol, stir at 400r / min for 2h, let it stand for 24h, transfer it to a centrifuge, and centrifuge at 8000r / min for 20min, collect the precipitate, and obtain the crude compound polysaccharide; weigh 2g of the crude product Add the complex polysaccharide to 40mL of deionized water, stir at 400r / min for 20min, then add 12mL of isopropanol, stir at 400r / min for 20min, then add 42mL of 20% sodium hydroxide...

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Abstract

The invention relates to a preparation method of an anti-freeze agent for marine products, and belongs to the technical field of marine product processing. According to the preparation method of the anti-freeze agent for marine products, carboxymethylation is conducted on protein and polysaccharide in a crude product complex polysaccharide, and combination of abundant inherent hydroxyl groups with some groups of protein molecules enables the protein molecules to be in a saturated state, thereby avoiding aggregation and denaturation of the protein molecules. Simultaneously, by the hydrogen-bond interaction of carboxymethyl groups stronger than that of the hydroxyl groups, the state of bound water embedded in the protein molecules is changed, and the bound water on the surfaces of the protein molecules is substituted by the carboxymethyl groups to be bound with the protein molecules, thereby playing a role of inhibiting denaturation of protein. Simultaneously, by enhancing the stability of water with the protein in the freezing process, formation of large ice crystals is reduced, thereby avoiding open-chain denaturation caused by spatial conformation change of fish protein, and effectively inhibiting freeze denaturation of the protein in the marine products. The anti-freeze agent has low sweetness and calorific value and has no influence on the flavor and nutritional values of the marine products.

Description

technical field [0001] The invention relates to a preparation method of an antifreeze agent for aquatic products, belonging to the technical field of aquatic product processing. Background technique [0002] Aquatic products are delicious, nutritious, and have strong health effects. However, because the water content of fresh aquatic products is about 70%, the tissues are fragile, the natural immune substances are less, the unsaturated fatty acids are easily oxidized, and the soluble protein content is high, so they are better than ordinary animals. Meat tissue is more prone to spoilage. At present, the most commonly used storage method is low-temperature freezing storage, which can effectively inhibit the reproduction of microorganisms, and at the same time reduce the biochemical reaction rate inside the material protein, thereby prolonging the shelf life of aquatic products, but long-term low-temperature freezing will lead to muscle growth in fish. Fibrillar protein denat...

Claims

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Application Information

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IPC IPC(8): A23B4/20
CPCA23B4/20A23V2002/00A23V2200/206A23V2250/51A23V2250/6414A23V2250/28
Inventor 吴义峰王之霖
Owner 江苏华友装饰工程有限公司
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