Physical method for selectively containing malignant degree of triple negative breast cancer cells

A triple-negative breast cancer and breast cancer cell technology, applied in the field of physics and biology, can solve the problems of violent plasma and unstable ionization, and achieve the effect of less dosage, less pain, and quicker effect.

Inactive Publication Date: 2017-11-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this device is that after adding oxygen to argon, the ionization becomes unstable, and the generated plasma is more violent, which is not suitable for the human body, and our experiments have proved that after adding oxygen, the effect of treating cancer cells is not as good as that of pure helium

Method used

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  • Physical method for selectively containing malignant degree of triple negative breast cancer cells
  • Physical method for selectively containing malignant degree of triple negative breast cancer cells
  • Physical method for selectively containing malignant degree of triple negative breast cancer cells

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Example 1: Treatment of breast cancer cells with a solution containing effective anti-cancer substances obtained by low-temperature plasma, and analysis of the results of detection of cancer cell proliferation effects.

[0021] The cancer cells and normal breast cells treated with the active medium prepared by low-temperature and normal-pressure plasma were added with CCK8 reagent, and then incubated in the incubator for 1-2 hours and then put into the microplate reader to detect cell proliferation. result image 3 (a) shows that under this condition, low temperature and atmospheric pressure plasma has no inhibitory effect on normal breast epithelial cells MCF10A, but weakly promotes cell growth. The same luminal A breast cancer cell MCF7 and normal breast epithelial cells MCF10A have the same Effect (the p-values ​​of treatment time of 1, 2, 3, 4, 5 minutes are 0.892, 0.194, 0.609, 0.494, 0.052, respectively, image 3 a), but it has a significant inhibitory effect on tripl...

Embodiment 2

[0022] Example 2: Treating breast cancer cells with a solution containing effective anti-cancer substances obtained by low-temperature plasma, showing the results of the detection of cancer cell migration effects.

[0023] In this experiment, 1,000,000 cells were evenly spread into a 6-well plate and incubated in an incubator for 24 hours. When the cells were evenly and densely attached to the bottom of the petri dish, scratch with a pipette, and a regular area without cells appeared at the bottom. , Then replace the original medium with the medium treated with low-temperature plasma, put it in the incubator, and take pictures of the cell morphology after 8, 16, and 24 hours, and finally calculate the area with the captured image The migration of cancer cells and normal cells. result Figure 4 It shows that the active medium prepared by low temperature and atmospheric pressure plasma can significantly and selectively prevent the migration of triple-negative breast cancer cells. ...

Embodiment 3

[0024] Example 3: Treatment of breast cancer cells with a solution containing effective anti-cancer substances obtained by low-temperature plasma, and analysis of the results of detection of cancer cell apoptosis effects.

[0025] In this experiment, 400,000 cells were evenly spread into a 6-well plate, incubated in an incubator for 24 hours, and replaced with a medium treated with low-temperature plasma, placed in the incubator for 24 hours, and then collected and placed Inflow tube, add AnnexinV and PI stains, and use AnnexinV / PI double staining method to detect cell apoptosis. AnnexinV has a high affinity with phosphatidylserine, so it can pass through exposed phosphatidylserine outside the cell and apoptosis The cell membrane binding of early cells is therefore used as a sensitive indicator for detecting early cell apoptosis. PI can penetrate the cell membrane to stain the nucleus red as a sensitive indicator for detecting late cell apoptosis. Therefore, matching Annexin V an...

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Abstract

The invention relates to a physical method for selectively containing the malignant degree of triple negative breast cancer cells. The physical method is characterized in that firstly, low-temperature normal-pressure helium plasmas are utilized to treat a culture medium to activate the culture medium, so that the culture medium carries with a large number of effective active substances; and then the breast cancer cells can be killed by means of the method that the active substances interact with the breast cancer cells through the treated culture medium, so that the effect on containing of the malignant degree of the triple negative breast cancer cells is achieved. According to the physical method for selectively containing the malignant degree of the triple negative breast cancer cells, the low-temperature normal-pressure helium plasmas are adopted so that the culture medium can generate the appropriate active oxygen substances, the containing effect on the triple negative breast cancer cells is achieved, and nearly no influence on normal cells is generated, so that high selectivity is achieved; and the breast cancer cells are treated through the indirect physical method, the convenient and efficient characteristics are achieved, and a culture medium solution subjected to plasma treatment can be treated as medical injection liquid to conduct injection therapy on breast cancer patients. According to the physical method, the triple negative breast cancer cells are safely, painlessly and efficiently killed without side effects.

Description

Technical field [0001] The invention relates to a culture medium containing plasma active substances to cultivate triple-negative breast cancer cells to cause their death. Mainly used in the direction of biopharmaceuticals, it is a new technology in the intersection of biology and physics. Background technique [0002] Breast cancer is a highly heterogeneous disease. According to the expression levels of commonly used immunohistochemical markers ER, PR, and HER2, it can be roughly divided into four subtypes, namely luminal A, luminal B, and HER2 positive. Triple negative. Among them, triple-negative breast cancer is the most malignant, and there is no effective targeted therapy drug due to the lack of surface receptors such as estrogen receptors. At present, the commonly used treatment methods still rely on traditional radiotherapy and chemotherapy. However, these methods have poor specificity to cancer cells because they also kill fast-growing normal cells, and have strong to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693
Inventor 戴晓峰徐潇宇肖少庆顾晓峰陆文怡
Owner JIANGNAN UNIV
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